A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants

The topic of this paper is the physiological adaptation of legume-rhizobium symbiotic plants to fluctuating and heterogeneous environmental constraints. How inter-organ signaling may contribute to whole plant adaptation to a localized stress is the major biological question addressed by this study using molecular and physiological approaches. The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation to a localized water constraint was analyzed using split-root systems. Results argue for a role of systemic N signaling in the root compensation of local variations impairing locally the symbiotic activity

A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants

The topic of this paper is the physiological adaptation of legume-rhizobium symbiotic plants to fluctuating and heterogeneous environmental constraints. How inter-organ signaling may contribute to whole plant adaptation to a localized stress is the major biological question addressed by this study using molecular and physiological approaches. The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation to a localized water constraint was analyzed using split-root systems. Results argue for a role of systemic N signaling in the root compensation of local variations impairing locally the symbiotic activity

A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants

The topic of this paper is the physiological adaptation of legume-rhizobium symbiotic plants to fluctuating and heterogeneous environmental constraints. How inter-organ signaling may contribute to whole plant adaptation to a localized stress is the major biological question addressed by this study using molecular and physiological approaches. The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation to a localized water constraint was analyzed using split-root systems. Results argue for a role of systemic N signaling in the root compensation of local variations impairing locally the symbiotic activity

Validation of a clinical practice-based algorithm for the diagnosis of autosomal recessive cerebellar ataxias based on NGS identified cases

International audienceEstablishing a molecular diagnosis of autosomal recessive cerebellar ataxias (ARCA) is challenging due to phenotype and genotype heterogeneity. We report the validation of a previously published clinical practice-based algorithm to diagnose ARCA. Two assessors performed a blind analysis to determine the most probable mutated gene based on comprehensive clinical and paraclinical data, without knowing the molecular diagnosis of 23 patients diagnosed by targeted capture of 57 ataxia genes and high-throughput sequencing coming from a 145 patients series. The correct gene was predicted in 61 and 78 % of the cases by the two assessors, respectively. There was a high inter-rater agreement [K = 0.85 (0.55-0.98) p < 0.001] confirming the algorithm's reproducibility. Phenotyping patients with proper clinical examination, imaging, biochemical investigations and nerve conduction studies remain crucial for the guidance of molecular analysis and to interpret next generation sequencing results. The proposed algorithm should be helpful for diagnosing ARCA in clinical practice