Environmental Education: A Chance for the Future

The U.S. Fish and Wildlife Service is committed to providing environmental education opportunities for the Nation\u27s student body. Local, regional and national educators are invited to investigate and utilize the resources of Back Bay National Wildlife Refuge with their colleagues and students. Teacher workshops at the Refuge provide orientation to Refuge lands, outdoor classroom sites, trails, interpretive facilities and equipment, and potential field activities. Refuge staff members are available to assist with: • Preliminary planning • Group scheduling • Library research • Workshop registration • Trip logistics and • On-site group orientation. Most classroom sites, associated trails/boardwalks, and Visitor Contact Station facilities are wheelchair accessible. Refuge habitats available for investigation include ocean, beach, pond, dune, bay, shrubland, maritime forest and marsh. During 1989, 3,700 students utilized the Refuge as an outdoor classroom

Rx for Success at Back Bay National Wildlife Refuge: Take Two Committed Partners-Add Water

As part of a major effort to improve habitats for waterfowl and other wetland-dependent wildlife, Ducks Unlimited, Inc. is contributing $187,500 in matching funds to help the U.S. Fish and Wildlife Service rehabilitate wetland impoundments at Back Bay NWR. The three-year project will increase Refuge management capabilities on existing wetland areas, create 300 acres of new marsh habitat and increase water management flexibility throughout the impoundment system. Components of the project include: Raising and re-sloping 8 miles of existing dikes Installing 13 new water control structures Constructing 6,000 feet of new dikes Creating two storage pools totalling 53 acres and Excavating 8 miles of water transport ditches. Surplus water will be made available to adjacent False Cape State Park/Barbours Hill Wildlife Management Area for the enhancement of 137 acres of waterfowl habitat. Benefits of the project will extend not only to migratory birds, but also the freshwater fish, amphibians, aquatic mammals, invertebrates and reptiles. Improved conditions for wildlife will also improve observation and educational opportunities for Refuge visitors. The project supports the goals of the North American Waterfowl Management Plan, an international strategy for the recovery of declining waterfowl populations

Refuge Land Acquisition: Helping Preserve Back Bay\u27s Wildlife Heritage

The once-renowned waterfowl populations and bass fishery of Back Bay, Virginia have declined dramatically in recent years. Lands surrounding Back Bay are increasingly threatened by on-going and potential land development. These lands serve as an important filter for pollutant and sediment-laden runoff from adjacent areas. The boundary of the Back Bay National Wildlife Refuge was expanded in 1989 to include an additional 6,340 acres of brackish marsh, forested swamp, and critical edge upland habitat, important to a variety of wildlife species and for its natural filtering effect. Within the U.S. Fish and Wildlife Service\u27s policy of working with willing sellers, the Refuge hopes to acquire and manage the land to improve its value to wildlife and reduce the amount of sediment and pollutants flowing into Back Bay. Refuge acquisition plans support the goals of the North American Waterfowl Management Plan, an international strategy for cooperation, to aid in the recovery of declining waterfowl populations. Refuge acquisition alone will not be enough to solve the current problems of the Back Bay resource; recovery is dependent on the cooperation and assistance of State and local governments, private organizations and individual citizens

Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens

Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the determination of 23 mycotoxins and phase I metabolites in DBSs from pigs and broiler chickens using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The targeted mycotoxins belong to groups for which the occurrence in feed is regulated by the European Union, namely, aflatoxins, ochratoxin A and several Fusarium mycotoxins, and to two groups of unregulated mycotoxins, namely Alternaria mycotoxins and Fusarium mycotoxins (enniatins and beauvericin). The impact of blood haematocrit, DBS sampling volume and size of the analysed DBS disk on the validation results was assessed. No effects of variation in size of the analysed disk, haematocrit and spotted blood volume were observed for most mycotoxins, except for the aflatoxins and beta-zearalanol (BZAL) at the lowest haematocrit (26%) level and for the enniatins (ENNs) at the lowest volume (40 mu L). The developed method was transferred to an LC-high resolution mass spectrometry instrument to determine phase II metabolites. Then, the DBS technique was applied in a proof-of-concept toxicokinetic study including a comparison with LC-MS/MS data from plasma obtained with conventional venous blood sampling. A strong correlation (r > 0.947) was observed between plasma and DBS concentrations. Finally, DBSs were also applied in a pilot exposure assessment study to test their applicability under field conditions

Protocol for the Reconstructing Consciousness and Cognition (ReCCognition) Study

Important scientific and clinical questions persist about general anesthesia despite the ubiquitous clinical use of anesthetic drugs in humans since their discovery. For example, it is not known how the brain reconstitutes consciousness and cognition after the profound functional perturbation of the anesthetized state, nor has a specific pattern of functional recovery been characterized. To date, there has been a lack of detailed investigation into rates of recovery and the potential orderly return of attention, sensorimotor function, memory, reasoning and logic, abstract thinking, and processing speed. Moreover, whether such neurobehavioral functions display an invariant sequence of return across individuals is similarly unknown. To address these questions, we designed a study of healthy volunteers undergoing general anesthesia with electroencephalography and serial testing of cognitive functions (NCT01911195). The aims of this study are to characterize the temporal patterns of neurobehavioral recovery over the first several hours following termination of a deep inhaled isoflurane general anesthetic and to identify common patterns of cognitive function recovery. Additionally, we will conduct spectral analysis and reconstruct functional networks from electroencephalographic data to identify any neural correlates (e.g., connectivity patterns, graph-theoretical variables) of cognitive recovery after the perturbation of general anesthesia. To accomplish these objectives, we will enroll a total of 60 consenting adults aged 20–40 across the three participating sites. Half of the study subjects will receive general anesthesia slowly titrated to loss of consciousness (LOC) with an intravenous infusion of propofol and thereafter be maintained for 3 h with 1.3 age adjusted minimum alveolar concentration of isoflurane, while the other half of subjects serves as awake controls to gauge effects of repeated neurobehavioral testing, spontaneous fatigue and endogenous rest-activity patterns

Biomarkers for exposure as a tool for efficacy testing of a mycotoxin detoxifier in broiler chickens and pigs

Applying post-harvest control measures such as adding mycotoxin detoxifying agents is a frequently-used mitigation strategy for mycotoxins. EFSA states that the efficacy of these detoxifiers needs to be tested using specific biomarkers for exposure. However, the proposed biomarkers for exposure are not further optimized for specific target species. Hence, the goal of this study was (a) to evaluate the most suitable biomarkers for deoxynivalenol (DON) and zearalenone (ZEN) in porcine plasma, urine and feces; and DON, aflatoxin B1 (AFB1) and ochratoxin A (OTA) in plasma and excreta of broiler chickens and (b) to determine the efficacy of a candidate detoxifier, as a proof-of-concept study. Therefore, a mixture of mycotoxins was administered as a single oral bolus with or without detoxifying agent. In accordance with literature AFB1, OTA, and DON-sulphate (DON-S) proved optimal biomarkers in broilers plasma and excreta whereas, in pigs DON-glucuronide (DON-GlcA) and ZEN-glucuronide (ZEN-GlcA) proved the optimal biomarkers in plasma, DON and ZEN-GlcA in urine and, ZEN in feces. A statistically significant reduction was seen between control and treatment group for both AFB1 and DON in broiler plasma, under administration of the mycotoxin blend and detoxifier dose studied suggesting thus, beneficial bioactivity

Implicit social associations for geometric-shape agents more strongly influenced by visual form than by explicitly identified social actions

Studies of infants' and adults' social cognition frequently use geometric-shape agents such as coloured squares and circles, but the influence of agent visual-form on social cognition has been little investigated. Here, although adults gave accurate explicit descriptions of interactions between geometric-shape aggressors and victims, implicit association tests for dominance and valence did not detect tendencies to encode the shapes’ social attributes on an implicit level. With regard to valence, the lack of any systematic implicit associations precludes conclusive interpretations. With regard to dominance, participants implicitly associated a yellow square as more dominant than a blue circle, even when the true relationship was the reverse of this and was correctly explicitly described by participants. Therefore, although explicit dominance judgements were strongly influenced by observed behaviour, implicit dominance associations were more clearly influenced by preconceived associations between visual form and social characteristics. This study represents a cautionary tale for those conducting experiments using geometric-shape agents