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Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold.
Effective use of plant biomass as an abundant and renewable feedstock for biofuel production and biorefinery requires efficient enzymatic mobilization of cell wall polymers. Knowledge of plant cell wall composition and architecture has been exploited to develop novel multifunctional enzymes with improved activity against lignocellulose, where a left-handed β-3-prism synthetic scaffold (BeSS) was designed for insertion of multiple protein domains at the prism vertices. This allowed construction of a series of chimeras fusing variable numbers of a GH11 β-endo-1,4-xylanase and the CipA-CBM3 with defined distances and constrained relative orientations between catalytic domains. The cellulose binding and endoxylanase activities of all chimeras were maintained. Activity against lignocellulose substrates revealed a rapid 1.6- to 3-fold increase in total reducing saccharide release and increased levels of all major oligosaccharides as measured by polysaccharide analysis using carbohydrate gel electrophoresis (PACE). A construct with CBM3 and GH11 domains inserted in the same prism vertex showed highest activity, demonstrating interdomain geometry rather than number of catalytic sites is important for optimized chimera design. These results confirm that the BeSS concept is robust and can be successfully applied to the construction of multifunctional chimeras, which expands the possibilities for knowledge-based protein design
Transarterial Embolization and Percutaneous Ethanol Injection as an Effective Bridge Therapy before Liver Transplantation for Hepatitis C-Related Hepatocellular Carcinoma
Background. Transarterial chemoembolization alone or in association with radiofrequency ablation is an effective bridging strategy for patients with hepatocellular carcinoma awaiting for a liver transplant. However, cost of this therapy may limit its utilization. This study was designed to evaluate the outcomes of a protocol involving transarterial embolization, percutaneous ethanol injection, or both methods for bridging hepatocellular carcinomas prior to liver transplantation. Methods. Retrospective review of all consecutive adult patients who underwent a first liver transplant as a treatment to hepatitis C-related hepatocellular carcinoma at our institution between 2002 and 2012. Primary endpoint was patient survival. Secondary endpoint was complete tumor necrosis. Results. Forty patients were analyzed, age 58 ± 7 years. There were 23 males (57.5%). Thirty-six (90%) out of the total 40 patients were within Milan criteria. Complete necrosis was achieved in 19 patients (47.5%). One-, 3-, and 5-year patient survival were, respectively, 87.5%, 75%, and 69.4%. Univariate analysis did not reveal any variable to impact on overall patient survival. Conclusions. Transarterial embolization, ethanol injection, or the association of both methods followed by liver transplantation comprises effective treatment strategy for hepatitis C-related hepatocellular carcinoma. This strategy should be adopted whenever transarterial chemoembolization and/or radiofrequency ablation are not available options
Acute effects of transcranial direct current stimulation (tDCS) on peak torque and 5000 m running performance: a randomized controlled trial
The benefits of transcranial direct current stimulation (tDCS) on brain function, cognitive response, and motor ability are well described in scientific literature. Nevertheless, the effects of tDCS on athletes’ performance remain unclear. To compare the acute effects of tDCS on the running performance of 5000 m (m) runners. Eighteen athletes were randomized into Anodal (n = 9) groups that received tDCS for 20 min and 2 mA, and Sham (n = 9), in the motor cortex region (M1). Running time in 5000 m, speed, perceived exertion (RPE), internal load and peak torque (Pt) were evaluated. The Shapiro–Wilk test followed by a paired Student’s t-test was used to compare Pt and total time to complete the run between the groups. The running time and speed of the Anodal group (p = 0.02; 95% CI 0.11–2.32; d = 1.24) was lower than the Sham group (p = 0.02, 95% CI 0.05–2.20; d = 1.15). However, no difference was found in Pt (p = 0.70; 95% CI − 0.75 to 1.11; d = 0.18), RPE (p = 0.23; 95% CI − 1.55 to 0.39; d = 0.60) and internal charge (p = 0.73; 95% CI − 0.77 to 1.09; d = 0.17). Our data indicate that tDCS can acutely optimize the time and speed of 5000 m runners. However, no alterations were found for Pt and RPE
Canopy characteristics and behavior of Nellore heifers in Brachiaria brizantha pastures under different grazing heights at a continuous stocking rate
Suplemento de difenil diselenuro inyectable en ovejas lecheras
Objective. The aim of this study was to evaluate the influence of subcutaneous supplementation with diphenyl diselenide ((PhSe)2) in dairy sheep infected with gastrointestinal nematodes on animal health and possible damage to environment when the feces of these animals will be used for fertilizing. Materials and methods. The experiment was performed using 16 primipara dairy sheep, that were divided into two groups: the group A as control and the group B supplemented with 3 µmol/kg of (PhSe)2 subcutaneously. Blood samples were used to determine the hepatic function, as well as the protein and lipid metabolism in animals. Feces were used to determine the number of helminths eggs per gram of feces (EPG), as well as used for ecotoxicology tests. Results. The (PhSe)2 supplementation not affected the helminths reproduction, since the EPG did not differ (p>0.05) between groups. Total protein and globulin levels increase (p<0.05) in supplemented animals, while the seric alanine aminotransferase (ALT) levels decrease (p<0.05) in the end of experimental design. Cholesterol levels increase (p<0.05) in the supplemented animals, while triglycerides, albumin and urea not differ between groups (p>0.05). The feces of supplemented sheep not interfered the springtails reproduction. Conclusions. At the administered dose, the (PhSe)2 is not able to control the parasitism, however, it did increase the globulins and cholesterol levels, that are important to immune response and for sheep reproduction, respectively. Also, the feces of supplemented animals with (PhSe)2 can be used as organic fertilizing, without negative impacts to environment.Objetivo. El objetivo de este estudio fue evaluar la influencia de la suplementación subcutánea con diselenuro de difenilo (PhSe)2 en ovejas lecheras infectadas con nematodos sobre la salud animal y posible daño al ambiente cuando las heces de estos animales se utilicen para fertilizar. Materiales y métodos. El experimento se realizó utilizando 16 ovejas lecheras, que se dividieron en dos grupos: el grupo A se usó como control y el grupo B se suplementó con 3 µmol/kg of (PhSe)2 vía subcutánea. Se utilizaron muestras de sangre para determinar la función hepática, así como el metabolismo de proteínas y lípidos en animales. Las heces se utilizaron para determinar el número de huevos por gramo de heces (EPG), así como para las pruebas de ecotoxicología. Resultados. La suplementación (PhSe)2 no afectó la reproducción de helmintos. Los niveles totales de proteína y globulina aumentan (p<0.05) en los animales suplementados, mientras que los niveles séricos de alanina aminotransferasa (ALT) disminuyen (p<0.05) al final del diseño experimental. Los niveles de colesterol aumentan (p<0.05) en los animales suplementados, mientras que los triglicéridos, la albúmina y la urea no difieren entre los grupos (p>0.05). Las heces de ovejas suplementadas no interferían en la reproducción de las colas de caballo. Conclusiones. A la dosis administrada, el (PhSe)2 no es capaz de controlar el parasitismo; sin embargo, aumenta los niveles de globulinas y colesterol, que son importantes para la respuesta inmune y para la reproducción, respectivamente. Las heces de animales suplementados pueden usarse como fertilizantes orgánicos, sin impactos negativos en el ambiente.
Increased biomass and salicylic acid elicitor activity in fennel (Foeniculum vulgare Miller)
Discovery of endangered annual killifish Austrolebias cheradophilus (Aplocheiloidei: Rivulidae) in Brazil, with comments on habitat, population structure and conservation status
Toxicidade diferencial de produtos à base de abamectina ao ácaro Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) em citros
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