3 research outputs found
Inhibicija rasta plijesni Aspergillus ochraceus ZMPBF 318 i Penicillum expansum ZMPBF 565 djelovanjem Äetiriju eteriÄnih ulja
Fungi produce a large variety of extracellular proteins, organic acids, and other metabolites and can adapt to several environmental conditions. Mycotoxin-producing moulds of the genera Aspergillus and Penicillium are common food contaminants. One of the natural ways to protect food from mould contamination is to use essential oils. In this study, we evaluated the effect of essential oils of cinnamon, lavender, rosemary, and sage at 1 % (v/v) concentration in yeast media inoculated with spores (fi nal concentration 106 mL-1 media) of Aspergillus ochraceus ZMPBF 318 and Penicillium expansum ZMPBF 565, alone or in combination, on fungal biomass. Cinnamon showed the best inhibitory effect (100 %). Lavender oil best inhibited the growth of Aspergillus ochraceus (nearly 100 %), and was less successful with Penicillium expansum (having dropped to 57 % on day 28). With cultivation time the inhibitory effect of sage and rosemary oil grew for Aspergillus ochraceus and dropped for Penicillium expansum. These results suggest that fungi can be controlled with essential oils, especially with cinnamon oil.Plijesni su poznate po svojoj visokoj sposobnosti proizvodnje razliÄitih izvanstaniÄnih proteina, organskih kiselina i drugih metabolita i po svojoj moguÄnosti prilagodbe na nepovoljne okoliÅ”ne uvjete, a primjenjuju se i u obradi otpadnih voda. Plijesni iz rodova Aspergillus i Penicillium Äesti su kontaminanti u hrani i posebno opasne jer tvore toksiÄne metabolite mikotoksine. EteriÄna ulja mogu se primijeniti kao prirodna sredstva za zaÅ”titu hrane od kontaminacije plijesnima. U radu su prikazani rezultati istraživanja utjecaja eteriÄnih ulja cimeta, lavande, ružmarina i kadulje na kontrolu rasta biomase plijesni Aspergillus ochraceus ZMPBF 318 i Penicillium expansum ZMPBF 565, u obliku Äistih i mijeÅ”anih kultura. Ulja su dodavana u koncentraciji od 1 % (v/v), a podloga (kvaÅ”Äev ekstrakt) bila je nacijepljena suspenzijama spora plijesni
(106 mL-1 podloge). EteriÄno ulje cimeta pokazalo je najveÄi inhibitorni uÄinak (100 %). Inhibitorni uÄinak eteriÄnog ulja lavande bio je veÄi na rast Aspergillusa ochraceusa (skoro 100 %) nego Penicilliuma expansuma (57 %). EteriÄna ulja kadulje i ružmarina pokazala su suprotne uÄinke. Inhibitorni uÄinak na Aspergillus ochraceus tijekom perioda uzgoja je rastao, a na Penicillium expansum opadao.
Rezultati pokazuju da se rast plijesni može kontrolirati primjenom eteriÄnih ulja, a posebno uljem cimeta. TakoÄer upuÄuju na ekonomsku vrijednost takvih tretmana
Antifungal effect of essential oils on growth of moulds biomass of Aspergillus ochraceus and Penicillium expansum
EteriÄna ulja i njihovi sastojci posjeduju antifungalni, antivirusni, antibakterijski, antiparazitski i insekticidni uÄinak. Danas se svojstva biljnih ulja i ekstrakta ili sastojaka eteriÄnih ulja primjenjuju u Å”irokom spektru ukljuÄujuÄi zaÅ”titu svježe i procesirane hrane, u farmaceutici, alternativnoj medicini i prirodnim terapijama. Svrha ovog rada bila je da se utvrdi antifungalni uÄinak eteriÄnih ulja kadulje, ružmarina, lavande i cimeta na rast plijesni Aspergillus ochraceus ZMPBF 318 i Penicillium expansum ZMPBF 565, potencijalnih producenata mikotoksina u tekuÄoj YES podlozi. Istraživanja su provedena u prisutnosti 10% (v/v) koncentracija razliÄitih eteriÄnih ulja na rast plijesni Aspergillus ochraceus i Penicillium expansum kao Äiste i mjeÅ”ovite kulture. Inhibicija rasta odabranih plijesni djelovanjem razliÄitih eteriÄnih ulja istraživana je gravimetrijski. Istraživana eteriÄna ulja inhibirala su rast plijesni. Vrlo dobar antifungalni uÄinak na istraživane plijesni imaju eteriÄna ulja cimeta i lavande, dok na djelovanje ulja kadulje i ružmarina plijesni pokazuju odreÄenu otpornost.Essential oils and their compounds have antifungal, antiviral, antibacterial, antiparasitic and insecticide effect. Today, the properties of plant oils and extracts or ingredients of essential oils are applied in a wide range, including the protection of fresh and processed food, pharmaceutics, alternative medicine and natural therapies. The purpose of this study was to determine the antifungal effects of essential oils of sage, rosemary, lavender and cinnamon on the growth of Aspergillus ochraceus ZMPBF 318 and Penicillium expansum ZMPBF 565, potential producers of mycotoxins in the current YES media. Research is conducted in the presence of 10% (v/v) concentration of various essential oils on the growth of molds Aspergillus ochraceus and Penicillium expansum as pure and mixed culture. The inhibition of mold growth, of selected various essential oils, was investigated gravimetrically. Studied essential oils have inhibitory effect on the growth of molds. Very good antifungal effect on the investigated molds have essential oils of cinnamon and lavender, while on the action of sage and rosemary oils molds have shown some resistance
Antifungal effect of essential oils on growth of moulds biomass of Aspergillus ochraceus and Penicillium expansum
EteriÄna ulja i njihovi sastojci posjeduju antifungalni, antivirusni, antibakterijski, antiparazitski i insekticidni uÄinak. Danas se svojstva biljnih ulja i ekstrakta ili sastojaka eteriÄnih ulja primjenjuju u Å”irokom spektru ukljuÄujuÄi zaÅ”titu svježe i procesirane hrane, u farmaceutici, alternativnoj medicini i prirodnim terapijama. Svrha ovog rada bila je da se utvrdi antifungalni uÄinak eteriÄnih ulja kadulje, ružmarina, lavande i cimeta na rast plijesni Aspergillus ochraceus ZMPBF 318 i Penicillium expansum ZMPBF 565, potencijalnih producenata mikotoksina u tekuÄoj YES podlozi. Istraživanja su provedena u prisutnosti 10% (v/v) koncentracija razliÄitih eteriÄnih ulja na rast plijesni Aspergillus ochraceus i Penicillium expansum kao Äiste i mjeÅ”ovite kulture. Inhibicija rasta odabranih plijesni djelovanjem razliÄitih eteriÄnih ulja istraživana je gravimetrijski. Istraživana eteriÄna ulja inhibirala su rast plijesni. Vrlo dobar antifungalni uÄinak na istraživane plijesni imaju eteriÄna ulja cimeta i lavande, dok na djelovanje ulja kadulje i ružmarina plijesni pokazuju odreÄenu otpornost.Essential oils and their compounds have antifungal, antiviral, antibacterial, antiparasitic and insecticide effect. Today, the properties of plant oils and extracts or ingredients of essential oils are applied in a wide range, including the protection of fresh and processed food, pharmaceutics, alternative medicine and natural therapies. The purpose of this study was to determine the antifungal effects of essential oils of sage, rosemary, lavender and cinnamon on the growth of Aspergillus ochraceus ZMPBF 318 and Penicillium expansum ZMPBF 565, potential producers of mycotoxins in the current YES media. Research is conducted in the presence of 10% (v/v) concentration of various essential oils on the growth of molds Aspergillus ochraceus and Penicillium expansum as pure and mixed culture. The inhibition of mold growth, of selected various essential oils, was investigated gravimetrically. Studied essential oils have inhibitory effect on the growth of molds. Very good antifungal effect on the investigated molds have essential oils of cinnamon and lavender, while on the action of sage and rosemary oils molds have shown some resistance