ChatBot Based on Language Modelling

Tato práce řeší využití jazykového modelování v chatbotovi pomocí neuronových sítí. Tento problém je řešen tak, že pomocí zpracování přirozeného jazyka je při vytváření odpovědi na uživatelskou zprávu prvně zpráva analyzována. Poté jsou vytvořeny počátky vět odpovědi, které jsou doplněny výstupem neuronové sítě. Dohromady tyto věty tvoří odpověď chatbota. Proběhlo porovnání s existujícím chatbotem Cleverbotem a byla zjišťována míra inteligence obou chatbotů, na jejichž základě jsou uvedeny možnosti dalšího vývoje.This paper addresses the use of language modeling using neural networks in the chatbot. Problem is solved by using natural language processing and first step of generating response based on user input is input analysiss. As next beginings of sentences are created which are completed by output of neural network. All created sentences form final chatbot response. There was a comparisson with chatbot Cleverbot and measure of intelligence for both chatbots was determined. Based on testing results, some techniques for future progress were concluded.

The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery

Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced during the DNA extraction process may result in inaccurate microbial representation. In this study, we attempted to find a DNA extraction protocol which could be effectively used to analyze both the bacterial and fungal community. We evaluated the effect of five DNA extraction methods (QIAamp DNA Stool Mini Kit, PureLinkTM Microbiome DNA Purification Kit, ZR Fecal DNA MiniPrepTM Kit, NucleoSpin® DNA Stool Kit, and IHMS protocol Q) on bacterial and fungal gut microbiome recovery using (i) a defined system of germ-free mice feces spiked with bacterial or fungal strains, and (ii) non-spiked human feces. In our experimental setup, we confirmed that the examined methods significantly differed in efficiency and quality, which affected the identified stool microbiome composition. In addition, our results indicated that fungal DNA extraction might be prone to be affected by reagent/kit contamination, and thus an appropriate blank control should be included in mycobiome research. Overall, standardized IHMS protocol Q, recommended by the International Human Microbiome Consortium, performed the best when considering all the parameters analyzed, and thus could be applied not only in bacterial, but also in fungal microbiome research