Effect of Controlled-release Fertilizer and Root-proof Capillary Wick Addition to Root-zone on Tomato Growth and Yield

This study was conducted to investigate the effects of controlled-release fertilizers and wick addition to the root-zone on plant growth and yield of medium-fruit sized tomato up to the 18(th) truss in long-term forcing culture. The set-up consisted of a box partitioned by a board into two halves, named box 1 and box 2. Box 1 contained 2.8 ℓ of growth medium (soil : bark : perlite : peat=2 : 2 : 1 : 1). Plant was grown in box 1 with a capillary wick, and at flowering of the 8(th) truss, the partition was removed and box 2 filled with 2.8 ℓ of the medium. There were two treatments, with or without capillary wick in box 2, namely, single wick (S) and double wicks (D). Plant height was greater in D than S in January, and the difference increased gradually thereafter. Flowering time of 18(th) truss in D was 10 days earlier and decapitated shoot weight was twice that of S. Fruit yield per plant was 8 kg in S and 9 kg in D with similar value of Brix and titratable acidity. There was no difference between S and D in dry root weight (7g/plant) or in xylem exudates (8ml/h), while in S in April plant growth was inferior, leaf color yellow greenish and fruit colour uneven towards the end of growth of plants. Xylem sap analysis showed that NO(3)-N was 10 me/ℓ in S and 6 me/ℓ in D. This concentration is weaker than that of Enshi standard nutrient solution generally used in hydroponics. These results suggest that application of controlled-release fertilizers and wick addition to box 2 with root-zone extended was effective for plant growth performance and fruit yield.中玉トマト18段摘心の長期促成栽培における肥効調節型肥料の適用と生育途中の根域拡張に伴う「紐」適用が生育と果実収量に及ぼす効果を調べた．栽培容器は可動仕切り板で2つに仕切り区画当たり容量は2.8ℓとし，第8段花房開花時に仕切り板をはずして5.6ℓ（1st Box＋2nd Box）とした．試験区は2区で2nd Boxに紐を配置しない1本区と2nd Boxにもう１本を配した2本区である．2本区の草丈は1月以降，1本区に比べ高く推移し第18段花房の開花日が約10日早まり，成長の指標とした摘心時の茎頂部新鮮重も1本区の2倍となった．1株の果実収量は1本区で8kg，2本区で9kgとなり果実の糖度，酸度には両区間に差はなかった．栽培終了時の根の乾物重と茎切断面から採取した木部いっ泌液は両区間に差はなかったが，両区とも栽培終期の4月には生育が衰え葉色は黄緑色で果実の着色も不均一であった．木部いっ泌の成分分析ではNO(3)-N濃度が1本区で10me/ℓ，2本区で6me/ℓであり，これらの濃度は一般に養液栽培に使用される園試標準濃度よりも低かった．以上の結果から，第8段花房開花時に仕切りを開放し新区画への土壌と肥料ならびに紐の新たな適用は，生育安定と収量確保に効果的に作用することが明らかとなった．しかし，紐の有無にかかわらず生育後期の生育が不良となったことから，今後は施肥設計の面から，とくにNO(3)-NとSO(4)-Sの両面から検討する必要があると考察した

Complete Genome Sequence of Pseudomonas amygdali pv. tabaci Strain 6605, a Causal Agent of Tobacco Wildfire Disease

Pseudomonas amygdali pv. tabaci strain 6605 is the bacterial pathogen causing tobacco wildfire disease that has been used as a model for elucidating virulence mechanisms. Here, we present the complete genome sequence of P. amygdali pv. tabaci 6605 as a circular chromosome from reads using a PacBio sequencer

T1DM complication in continuous insulin

To evaluate whether continuous subcutaneous insulin infusion attenuates the progression of diabetic complications, we retrospectively extracted data from 35 individuals who had developed type 1 diabetes mellitus aged ≤20 years and whose treatment had been changed from multiple daily injections to continuous subcutaneous insulin infusion. The annual changes in estimated glomerular filtration rate, urinary albumin excretion rate, carotid intima-media thickness and brachial-ankle pulse wave velocity during each treatment period were calculated. Although mean glycated hemoglobin under the continuous subcutaneous insulin infusion treatment was lower than that under the multiple daily injection treatment, there were no significant differences in annual changes in diabetic nephropathy and atherosclerosis between the two treatment periods. This pilot study showed that, in Japanese patients with juvenile-onset type 1 diabetes mellitus, there was no significant difference in the progression of diabetic nephropathy and atherosclerosis, at least in the early stage, between the two treatments

Dose-dependent effects of fat

Humans have a high preference for fat, and its excessive intake leads to obesity. This study aimed to investigate the effects of dose-dependent fat intake on biological responses and postprandial appetite sensation in healthy adult subjects. Age and body mass index were 29 ± 1 years and 21.1 ± 0.4 kg/m2, respectively. We conducted a randomized, crossover trial and measured laboratory data and appetite sensation via the visual analog scale. Each participant was provided with four different test meals. They consisted of common, basic foods and contained 75g liquid glucose and 4 slices of crackers to which 0 g butter (control), 10 g butter (B10), 20 g butter (B20), and 40 g butter (B40) were added, respectively. The results indicated that single ingestion of butter did not influence laboratory values of glucose, insulin, glucose-dependent insulinotropic polypeptide (GIP), total bile acids, or high-sensitivity CRP (hs-CRP). Regarding postprandial appetite sensation, appetite ratings for fullness were the highest after the B40 meal (p < 0.05) ; however, satisfaction ratings were not significantly different after the ingestion of this meal. Ratings were significantly different after the B20 meal. In conclusion, healthy adult subjects experienced fullness and satisfaction after ingesting 20-40 g of butter

脂質摂取の多い食習慣とたんぱく質及び甘い食べ物に対する欲求との関連

Reducing dietary calorie density (CD) is useful in body weight management. This study investigates the association between dietary habits and preferences for different CDs. We conducted a randomized crossover study of 232 healthy subjects who consumed packed lunch boxes containing a control, high-meat and low-rice, low-vegetable, medium-fat and low-vegetable, high-fat, and high-fat and low-vegetable meals over six sessions. The subjective levels of sensory properties were assessed over time using a visual analog scale and the area under the curve. Subjects were assessed for dietary habits using a brief-type self-administered diet history questionnaire (BDHQ) and were divided into two groups based on a daily fat energy ratio≥25% (high fat [HF], n=116) and <25% (normal, n=116) that was matched for age, body mass index, and sex ratio. Our findings indicate that the desire for sweetness was higher in the HF group than in the normal group, regardless of the meals consumed. Particularly, among the 500-kcal low-CD meals, a high-protein meal provided greater fullness and satisfaction and lower prospective consumption in the HF group than in the normal group. Therefore, our study demonstrates that postprandial appetite sensation is associated with dietary habits of fat intake

Possible Existence of Lysosome-Like Organella within Mitochondria and Its Role in Mitochondrial Quality Control

The accumulation of unhealthy mitochondria results in mitochondrial dysfunction, which has been implicated in aging, cancer, and a variety of degenerative diseases. However, the mechanism by which mitochondrial quality is regulated remains unclear. Here, we show that Mieap, a novel p53-inducible protein, induces intramitochondrial lysosome-like organella that plays a critical role in mitochondrial quality control. Mieap expression is directly regulated by p53 and is frequently lost in human cancer as result of DNA methylation. Mieap dramatically induces the accumulation of lysosomal proteins within mitochondria and mitochondrial acidic condition without destroying the mitochondrial structure (designated MALM, for Mieap-induced accumulation of lysosome-like organelles within mitochondria) in response to mitochondrial damage. MALM was not related to canonical autophagy. MALM is involved in the degradation of oxidized mitochondrial proteins, leading to increased ATP synthesis and decreased reactive oxygen species generation. These results suggest that Mieap induces intramitochondrial lysosome-like organella that plays a critical role in mitochondrial quality control by eliminating oxidized mitochondrial proteins. Cancer cells might accumulate unhealthy mitochondria due to p53 mutations and/or Mieap methylation, representing a potential cause of the Warburg effect