Hairy vetch cover crop affects weed diversity and composition in no-till sunflower

Vicia villosa Roth, or hairy vetch, is one of the most productive cover crops (CC) in Mediterranean dry areas, with an interesting potential as dead mulch in no-tillage cropping systems. In low-input cropping systems, mechanical termination of hairy vetch could replace a glyphosate application to control weeds. Mechanical termination (e.g. with a roller crimper) of hairy vetch is ineffective until the late growing stages of vetch, leading to delayed sowing and yield reduction in the following crop. To find the best compromise in timing between effective termination of vetch and reasonable yield of no-till sunflower, an on-farm experiment was carried out in Pisa (Italy) in 2012/13 and 2013/14 to compare three termination techniques (roller crimper with no, half and full dose of glyphosate) and three termination dates (before flowering, beginning of flowering, 70% of flowering of vetch). Data on weed biomass, species density and soil cover were collected at CC termination and during sunflower growth. Weed diversity and composition were analyzed through ANOVA, PERMANOVA and NMDS

Can Shading Affect Nitrogen Fixation Of Forage Legume Swards? An Assessment Of B-Value Through The 15N Natural Abundance Method

Tree-based intercropping systems are gaining pace as a land-use strategy to cope with climate change and provide environmental, economic, and social benefits. The integration of nitrogen-fixing crops between trees can be a solution to increase the land productivity and reduce the reliance on external inputs by increasing nitrogen (N) availability and then both tree and crop growth. Intercropping perennial legumes with trees can also reduce nitrogen losses, due to the higher amount of N accumulated in stable forms in the soil due to biological N₂-fixation and N root compartimentation. On the other hand tree competition for light, water and nutrients eventually could limit legume growth and N₂-fixation. The isotopic method based on ¹⁵N natural abundance is one of the most used methods to assess Biological Nitrogen Fixation (BNF). The B-value, that is defined as the δ¹⁵N value of a legume when completely dependent on N₂-fixation for satisfying its N demand, is of primary importance for BNF estimations. The B-value may vary with species, plant age at harvest and growing conditions, e.g. light availability. Therefore, the B-value found in literature could not be representative for all legumes and environments, in particular for legumes grown intercropped with trees and thus subject to shading conditions. In this pot experiment we assessed the B-value of several forage legumes, as affected by different levels of simulated shading and grown in N-free medium. A greenhouse pot experiment was established in March 2021 at the Department of Agriculture, Food and Environment (DAFE) of the University of Pisa to determine the B-value for ¹⁵N calculations about two forage legume species grown in a field trial located at the Center of Agri-Envirnomental Research “Enrico Avanzi” of the University of Pisa, San Piero a Grado (Pisa) (43°41'6.97"N 10°20'29.22"E), using the same shade treatments

Ground Beetle (Coleoptera: Carabidae) Assemblages and Slug Abundance in Agricultural Fields Under Organic and Low-Input Conventional Management Within a Long-Term Agronomic Trial in Central Italy

Inside a long-term agronomic trial aimed at evaluating the effects of organic and low-input conventional management systems on soil fertility and arable crop production, we selected six fields bordered by hedgerows, three under each management system. Here, we analyzed the carabid assemblages and the slug abundance. Samplings took place in five different periods, across 1 yr of observations. The carabid abundances were similar in organic and conventional fields. The Shannon–Wiener diversity index (H’) showed a higher value in the conventional fields, although in the organic fields, a higher number of species were observed. The multivariate analysis described similar carabid communities, but excluding the period factor, it showed a significant influence of the management system. There was no difference between the captures of traps placed along the hedgerow and in the middle, whereas in the conventional fields, the hedgerow traps captured a higher number of specimens, showing a role of the hedgerow as carabid reservoir. The slugs were present mainly while green manure was grown on the organic fields where also Poecilus cupreus Linné, 1758 (Coleoptera: Carabidae) was captured abundantly

Substance use and psychiatric symptoms in subjects referred to the Drug Addiction Services by the Prefecture

n/

Light reduction affected agronomic performance and nutritive value of temporary grassland swards in a Mediterranean rainfed plot trial

In Italy, traditional olive orchards are characterised by low tree density (100-300 ha-1) allowing the cultivation of forage and crops under the tree canopy (Paris et al., 2019). Eichhorn et al. (2006), reported that in Central Italy there are 20000 ha of farmland identified as a silvoarable olive orchard. The intercropping of perennial legumes and trees is a key strategy to improve nutrient cycle of silvoarable systems, due to the higher amount of nitrogen (N) accumulated in stable forms in soil due by biological nitrogen-fixation (Hernandez-Esteban et al., 2019; Sanna et al., 2019), leading to a request for reduction of inorganic N fertilisation. Perennial legumes can also provide a continuous soil cover during the entire year reducing soil loss risk (Vallebona et al., 2016). In the Mediterranean basin, the most important perennial legume is alfalfa (Medicago sativa L.). Previous studies reported that alfalfa nutritive value was not negatively affected by tree presence (Mantino et al., 2021), whereas legume production was reduced due the competition for resources such as water (Nasielski et al., 2015), nutrients (Isaac et al., 2014) and light (Mantino et al., 2021). In Tuscany, sulla (Hedysarum coronarium L.) an autochthonous biennial legume is appreciated for its rusticity, productivity, and quality and it is intercropped with Italian ryegrass (Lolium multiflorum Lam.) for a better utilisation as pasture. In 2019, a rainfed field plot trial was established to evaluate agronomic performance and nutritive value of different perennial forage species grown under different levels of light reduction, aiming to start a selection of shade tolerant forage crops. In October, the plot trial was established in Pisa, on a clay-loam soil with pH of 8.1 and 2.5 % w/w of organic matter content in the topsoil (0-0.3 m). Before sowing, 100 kg ha-1 of P2O5 were applied. The experimental layout complies with a two-factor randomized complete block design with four replicates (18 m2 sizing each plot). The first factor included five different swards: i) sulla cv. Silvan, (ii) ryegrass cv. Teanna, (iii) mix of sulla cv. Silvan and ryegrass, 50:50 (iv) mix of sulla cv. Silvan, sulla cv. Chiara Stella and sulla cv. Bellante 33:33:33 and (v) alfalfa cv. Messe. The second factor included three increasing shading levels: S0) the control representing full light availability, S25) and S50), corresponding to a reduction of potential light availability of 25 and 50% respectively. As previously tested by Varella et al. (2011), shading was provided by woody slats, N-S oriented, 2.0 m long and 0.10 m wide, with a distance between each slat of 0.10 m for S50 and 0.20 m for S25, covering a total surface of 4 m2. After sowing, slats were placed at 0.8 m above ground level. Yield and nutritive value of herbage mass and N2 fixation were evaluated for two consecutive years. Herbage biomass was not affected by the reduction of the 50% of light in ryegrass and ryegrass-sulla mixture while it was negatively affected in alfalfa and sulla. Conversely, the 25% of shade level had no effect on legume yield

Evaluation of the Agronomic Performance of Organic Processing Tomato as Affected by Different Cover Crop Residues Management

No‐till practices reduce soil erosion, conserve soil organic carbon, and enhance soil fertility. Yet, many factors could limit their adoption in organic farming. The present study investigated the effects of tillage and cover cropping on weed biomass, plant growth, yield, and fruit quality of an organic processing tomato (Solanum lycopersicon L. var. Elba F1) over two seasons (2015–2017). We compared systems where processing tomato was transplanted on i) tilled soil following or not a winter cover crop (Trifolium squarrosum L.) and with/without a biodegradable plastic mulch; and ii) no‐till where clover was used, after rolling and flaming, as dead mulch. Tomato in no‐till suffered from high weed competition and low soil nitrogen availability leading to lower plant growth, N uptake, and yield components with respect to tilled systems. The total yield in no‐till declined to 6.8 and 18.3 t ha−1 in 2016 and 2017, respectively, with at least a 65% decrease compared to tilled clover‐based systems. No evidence of growth‐limiting soil compaction was noticed but a slightly higher soil resistance was in the no‐till topsoil. Tillage and cover crop residues did not significantly change tomato quality (pH, total soluble solids, firmness). The incorporation of clover as green manure was generally more advantageous over no‐till. This was partly due to the low performance of the cover crop where improvement may limit the obstacles (i.e., N supply and weed infestation) and enable the implementation of no‐till in organic vegetable systems

Electro-magnetic field promotes osteogenic differentiation of BM-hMSCs through a selective action on Ca(2+)-related mechanisms

Exposure to Pulsed Electromagnetic Field (PEMF) has been shown to affect proliferation and differentiation of human mesenchymal stem cells derived from bone marrow stroma (BM-hMSC). These cells offer considerable promise in the field of regenerative medicine, but their clinical application is hampered by major limitations such as poor availability and the time required to differentiate up to a stage suitable for implantation. For this reason, several research efforts are focusing on identifying strategies to speed up the differentiation process. In this work we investigated the in vitro effect of PEMF on Ca2+-related mechanisms promoting the osteogenic differentiation of BM-hMSC. Cells were daily exposed to PEMF while subjected to osteogenic differentiation and various Ca2+-related mechanisms were monitored using multiple approaches for identifying functional and structural modifications related to this process. The results indicate that PEMF exposure promotes chemically induced osteogenesis by mechanisms that mainly interfere with some of the calcium-related osteogenic pathways, such as permeation and regulation of cytosolic concentration, leaving others, such as extracellular deposition, unaffected. The PEMF effect is primarily associated to early enhancement of intracellular calcium concentration, which is proposed here as a reliable hallmark of the osteogenic developmental stage

Microplastics ingestion in the ephyra stage of Aurelia sp. triggers acute and behavioral responses

For the first time, we report a correspondence between microplastics (MP) ingestion and ecotoxicological effects in gelatinous zooplankton (Cnidarian jellyfish). The ephyra stage of the jellyfish Aurelia sp. was exposed to both environmental and high concentrations of fluorescent 1–4 μm polyethylene MP (0.01–10 mg/L). After 24 and 48 h, MP accumulation, acute (Immobility) and behavioral (Frequency pulsation) endpoints were investigated. MP were detected by confocal and tomographic investigations on gelatinous body and mouth, either attached on the surface or ingested. This interaction was responsible for impairing ephyrae survival and behavior at all tested concentrations after 24 h. Acute and behavioral effects were also related to mechanical disturbance, caused by MP, triggering a loss of radial symmetry. Contaminated ephyrae exposed to clean seawater showed full recovery after 72 h highlighting the organisms without the microspheres, attached on body jellyfish surface around the mouth and lappets. In conclusion, short-term exposure to MP affects ephyrae jellyfish health, impairing both their survival and behavior. Polyethylene MP temporarily affect both Immobility and Frequency of pulsation of Aurelia sp. jellyfish. This study provides a first step towards understanding and clarifying the potential impacts of MP contamination in gelatinous zooplankton

Development of label-free biophysical markers in osteogenic maturation

The spatial and temporal changes of morphological and mechanical properties of living cells reflect complex functionally-associated processes. Monitoring these modifications could provide a direct information on the cellular functional state. Here we present an integrated biophysical approach to the quantification of the morphological and mechanical phenotype of single cells along a maturation pathway. Specifically, quantitative phase microscopy and single cell biomechanical testing were applied to the characterization of the maturation of human foetal osteoblasts, demonstrating the ability to identify effective label-free biomarkers along this fundamental biological process

Effectiveness of a phone-based nurse monitoring assessment and intervention for chemotherapy-related toxicity: A randomized multicenter trial

PurposeAnticancer treatment-related toxicities can impact morbidity and mortality, hamper the administration of treatment, worsen the quality of life and increase the burden on the healthcare system. Therefore, their prompt identification is crucial. NICSO (Italian Network for Supportive Care in Cancer) conducted a nationwide randomized trial to evaluate the role of a planned, weekly phone-based nurse monitoring intervention to prevent and treat chemotherapy, targeted therapy- and immunotherapy-related toxicities. Here, we report the results from the chemotherapy arm. MethodsThis was a nationwide, randomized, open-label trial conducted among 29 Italian centers (NCT04726020) involving adult patients with breast, colon, or lung cancer and a life expectancy >= 6 months receiving adjuvant chemotherapy. Patients received either a weekly nurse monitoring phone call and an educational leaflet reporting practical advice about prevention and treatment of toxicities (experimental group) or the educational leaflet only (control group). ResultsThe addition of a nurse monitoring intervention may help reduce time spent with severe toxicities (grade >= 3), particularly those less frequently reported in clinical practice, such as fatigue. When considering grade 1-2 AEs, times with mild/moderate diarrhea, mucositis, fatigue and pain were shorter in the experimental arm. Time spent without AEs was significantly longer in the experimental arms for all the toxicities. The requirement for special medical attention was comparable between groups. ConclusionThis study suggests the need for implementing a better system of toxicity assessment and management for patients treated with adjuvant chemotherapy to promote effective preventive and/or therapeutic intervention against these events