Uso de células estromales mesenquimales derivadas de la gelatina de Wharton para el tratamiento de uveítis recurrente equina: estudio piloto

Equine recurrent uveitis (ERU) is a disease that affects 2 to 25 % of equines worldwide, 56% of which go blind; therefore, it is considered the most common cause of blindness in horses. ERU is a spontaneous immune-mediated condition characterized by recurrent intraocular inflammatory events. Currently, there is no treatment for horses with this disease. Mesenchymal stromal cells (MSCs) derived from various tissues, such as Wharton's jelly (WJ), have demonstrated their ability to modulate the immune response by negatively regulating the inflammatory process. The objective of this pilot study was to evaluate the effect of using MSCs derived from WJ as a treatment for ERU. The WJ was obtained and processed according to previously described methodologies for obtaining EMF. The horses involved in this study received a dose of 5x106 MSCs in the subpalpebral area. The research evaluated the concentration of interleukins (IL: IL-1a, IL-2, IL-10, IFN-g, and TNFa) in tear samples obtained before treatment inoculation, 30 min after the inoculation, and 7 days post inoculation. No significant changes in IL concentration were observed suggesting a decrease in pro-inflammatory ILs. However, horses with ERU treated with MSCs exhibited a positive response to therapy, evidenced by a decrease in signs of ERU. The results obtained suggest that treatment of ERU with WJ-derived MSCs is a safe alternative with promising results.La uveítis recurrente equina (URE) es una enfermedad que afecta del 2 al 25 % de los equinos a nivel mundial, de los cuales el 56 % se quedan ciegos; por lo tanto, es considerada la causa más común de ceguera en caballos. La URE es un padecimiento espontáneo inmunomediado caracterizado por eventos recurrentes de inflamación intraocular. Actualmente, no existe tratamiento para los caballos con esta enfermedad. Las células estromales mesenquimales (CEM) derivadas de diversos tejidos, como la gelatina de Wharton (GW), han demostrado su capacidad de modular la respuesta inmune al regular negativamente el proceso inflamatorio. El objetivo del presente estudio piloto fue el evaluar el efecto del uso de CEM derivadas de GW como tratamiento para la URE. La GW se obtuvo y procesó con base en metodologías previamente descritas para la obtención de CEM. Los caballos involucrados en este estudio recibieron una dosis de 5x106 CEM en la zona subpalpebral. Se evaluó la concentración de interleucinas (IL) (IL-1a, IL-2, IL-10, IFN-g y TNFa) en muestras de lágrima obtenidas antes de la inoculación del tratamiento, 30 min después y 7 días post inoculación. No se observaron cambios significativos en la concentración de IL que sugieran la disminución de IL proinflamatorias. Sin embargo, los caballos con URE tratados con CEM mostraron una respuesta positiva a la terapia, evidenciada por la disminución en la signología de la URE. Los resultados obtenidos sugieren que el tratamiento de la URE con CEM derivadas de GW es una alternativa segura con resultados prometedores

Ethnoveterinary practices used by owners of working equids with dermal disorders from tropical regions of Veracruz, Mexico

344-350The tropical environmental conditions of the North-central region of the state of Veracruz, Mexico, are favorable for the development of specific dermal disorders in working equids and sometimes cost and inaccessibility has led to the continuation as well as rediscovery of traditional treatment practices in developing countries. Four hundred sixty seven working equids from the North-central region of the state of Veracruz, Mexico, were treated at the Donkey Sanctuary Mobile Clinic Program-Universidad Nacional Autónoma de México. Animals with signs of cutaneous pathology underwent a physical examination. Each owner of an animal with dermal disorders was interviewed using a standardized structured and pre-tested questionnaire that included detailed questions on owner demographics and equid ownership, feeding practices, animal disease history and the ethnoveterinay practices (EVP) performed. Prevalence (P) and confidence intervals for prevalence (CIp) for husbandry and EVPs were calculated. A total of 84 owners who cared for 96 equids with dermal disorders were interviewed. Nine dermal disorders were reported by owners. The therapeutic methods used included gentian violet, hydrogen peroxide, soap, commercial parasiticides, used engine oil, dehorning paste, sulfur, creolin, and practices such as suture and firing or branding. Only the medicinal plant Aloe vera was reported as being used to treat skin wounds. </span