6,707 research outputs found
Relationship between incommensurability and superconductivity in Peierls distorted charge-density-wave systems
We study the pairing potential induced by fluctuations around a
charge-density wave (CDW) with scattering vector Q by means of the Froehlich
transformation. For general commensurability M, defined as |k+M*Q>=|k>, we find
that the intraband pair scattering within the M subbands scales with M whereas
the interband pair scattering becomes suppressed with increasing CDW order
parameter. As a consequence superconductivity is suppressed when the Fermi
energy is located between the subbands as it is usually the case for nesting
induced CDW's, but due to the vertex renormalization it can be substantially
enhanced when the chemical potential is shifted sufficiently inside one of the
subbands. The model can help to understand the experimentally observed
dependence of the superconducting transition temperature from the stripe phase
incommensurability in the lanthanum cuprates.Comment: 6 pages, 3 figure
Ohta-Jasnow-Kawasaki Approximation for Nonconserved Coarsening under Shear
We analytically study coarsening dynamics in a system with nonconserved
scalar order parameter, when a uniform time-independent shear flow is present.
We use an anisotropic version of the Ohta-Jasnow-Kawasaki approximation to
calculate the growth exponents in two and three dimensions: for d=3 the
exponents we find are the same as expected on the basis of simple scaling
arguments, that is 3/2 in the flow direction and 1/2 in all the other
directions, while for d=2 we find an unusual behavior, in that the domains
experience an unlimited narrowing for very large times and a nontrivial
dynamical scaling appears. In addition, we consider the case where an
oscillatory shear is applied to a two-dimensional system, finding in this case
a standard t^1/2 growth, modulated by periodic oscillations. We support our
two-dimensional results by means of numerical simulations and we propose to
test our predictions by experiments on twisted nematic liquid crystals.Comment: 25 RevTeX pages, 7 EPS figures. To be published in Phys. Rev.
Truncated and Helix-Constrained Peptides with High Affinity and Specificity for the cFos Coiled-Coil of AP-1
Protein-based therapeutics feature large interacting surfaces. Protein folding endows structural stability to localised surface epitopes, imparting high affinity and target specificity upon interactions with binding partners. However, short synthetic peptides with sequences corresponding to such protein epitopes are unstructured in water and promiscuously bind to proteins with low affinity and specificity. Here we combine structural stability and target specificity of proteins, with low cost and rapid synthesis of small molecules, towards meeting the significant challenge of binding coiled coil proteins in transcriptional regulation. By iteratively truncating a Jun-based peptide from 37 to 22 residues, strategically incorporating i-->i+4 helix-inducing constraints, and positioning unnatural amino acids, we have produced short, water-stable, alpha-helical peptides that bind cFos. A three-dimensional NMR-derived structure for one peptide (24) confirmed a highly stable alpha-helix which was resistant to proteolytic degradation in serum. These short structured peptides are entropically pre-organized for binding with high affinity and specificity to cFos, a key component of the oncogenic transcriptional regulator Activator Protein-1 (AP-1). They competitively antagonized the cJun–cFos coiled-coil interaction. Truncating a Jun-based peptide from 37 to 22 residues decreased the binding enthalpy for cJun by ~9 kcal/mol, but this was compensated by increased conformational entropy (TDS ≤ 7.5 kcal/mol). This study demonstrates that rational design of short peptides constrained by alpha-helical cyclic pentapeptide modules is able to retain parental high helicity, as well as high affinity and specificity for cFos. These are important steps towards small antagonists of the cJun-cFos interaction that mediates gene transcription in cancer and inflammatory diseases
Report and recommendations on multimedia materials for teaching and learning electricity and magnetism
This paper presents the results of a peer review of multimedia materials for teaching and learning electricity and magnetism prepared as a part of the annual activities undertaken by an international group of scientists associated with Multimedia Physics in Teaching and Learning. The work promotes the use of valuable and freely accessible information technology materials for different levels of teaching, mostly higher education. The authors discuss the process of selecting resources and the rubrics used in the rating process. The reviews of high-quality learning resources are presented along with descriptions of valuable didactical feature
Vertical transport and electroluminescence in InAs/GaSb/InAs structures: GaSb thickness and hydrostatic pressure studies
We have measured the current-voltage (I-V) of type II InAs/GaSb/InAs double
heterojunctions (DHETs) with 'GaAs like' interface bonding and GaSb thickness
between 0-1200 \AA. A negative differential resistance (NDR) is observed for
all DHETs with GaSb thickness 60 \AA below which a dramatic change in the
shape of the I-V and a marked hysteresis is observed. The temperature
dependence of the I-V is found to be very strong below this critical GaSb
thickness. The I-V characteristics of selected DHETs are also presented under
hydrostatic pressures up to 11 kbar. Finally, a mid infra-red
electroluminescence is observed at 1 bar with a threshold at the NDR valley
bias. The band profile calculations presented in the analysis are markedly
different to those given in the literature, and arise due to the positive
charge that it is argued will build up in the GaSb layer under bias. We
conclude that the dominant conduction mechanism in DHETs is most likely to
arise out of an inelastic electron-heavy-hole interaction similar to that
observed in single heterojunctions (SHETs) with 'GaAs like' interface bonding,
and not out of resonant electron-light-hole tunnelling as proposed by Yu et al.
A Zener tunnelling mechanism is shown to contribute to the background current
beyond NDR.Comment: 8 pages 12 fig
Spin fluctuations in nearly magnetic metals from ab-initio dynamical spin susceptibility calculations:application to Pd and Cr95V5
We describe our theoretical formalism and computational scheme for making
ab-initio calculations of the dynamic paramagnetic spin susceptibilities of
metals and alloys at finite temperatures. Its basis is Time-Dependent Density
Functional Theory within an electronic multiple scattering, imaginary time
Green function formalism. Results receive a natural interpretation in terms of
overdamped oscillator systems making them suitable for incorporation into spin
fluctuation theories. For illustration we apply our method to the nearly
ferromagnetic metal Pd and the nearly antiferromagnetic chromium alloy Cr95V5.
We compare and contrast the spin dynamics of these two metals and in each case
identify those fluctuations with relaxation times much longer than typical
electronic `hopping times'Comment: 21 pages, 9 figures. To appear in Physical Review B (July 2000
Homochirality and the need of energy
The mechanisms for explaining how a stable asymmetric chemical system can be
formed from a symmetric chemical system, in the absence of any asymmetric
influence other than statistical fluctuations, have been developed during the
last decades, focusing on the non-linear kinetic aspects. Besides the absolute
necessity of self-amplification processes, the importance of energetic aspects
is often underestimated. Going down to the most fundamental aspects, the
distinction between a single object -- that can be intrinsically asymmetric --
and a collection of objects -- whose racemic state is the more stable one --
must be emphasized. A system of strongly interacting objects can be described
as one single object retaining its individuality and a single asymmetry; weakly
or non-interacting objects keep their own individuality, and are prone to
racemize towards the equilibrium state. In the presence of energy fluxes,
systems can be maintained in an asymmetric non-equilibrium steady-state. Such
dynamical systems can retain their asymmetry for times longer than their
racemization time.Comment: 8 pages, 7 figures, submitted to Origins of Life and Evolution of
Biosphere
Prospecting environmental mycobacteria: combined molecular approaches reveal unprecedented diversity
Background: Environmental mycobacteria (EM) include species commonly found in various terrestrial and aquatic environments, encompassing animal and human pathogens in addition to saprophytes. Approximately 150 EM species can be separated into fast and slow growers based on sequence and copy number differences of their 16S rRNA genes. Cultivation methods are not appropriate for diversity studies; few studies have investigated EM diversity in soil despite their importance as potential reservoirs of pathogens and their hypothesized role in masking or blocking M. bovis BCG vaccine.
Methods: We report here the development, optimization and validation of molecular assays targeting the 16S rRNA gene to assess diversity and prevalence of fast and slow growing EM in representative soils from semi tropical and temperate areas. New primer sets were designed also to target uniquely slow growing mycobacteria and used with PCR-DGGE, tag-encoded Titanium amplicon pyrosequencing and quantitative PCR.
Results: PCR-DGGE and pyrosequencing provided a consensus of EM diversity; for example, a high abundance of pyrosequencing reads and DGGE bands corresponded to M. moriokaense, M. colombiense and M. riyadhense. As expected pyrosequencing provided more comprehensive information; additional prevalent species included M. chlorophenolicum, M. neglectum, M. gordonae, M. aemonae. Prevalence of the total Mycobacterium genus in the soil samples ranged from 2.3×107 to 2.7×108 gene targets g−1; slow growers prevalence from 2.9×105 to 1.2×107 cells g−1.
Conclusions: This combined molecular approach enabled an unprecedented qualitative and quantitative assessment of EM across soil samples. Good concordance was found between methods and the bioinformatics analysis was validated by random resampling. Sequences from most pathogenic groups associated with slow growth were identified in extenso in all soils tested with a specific assay, allowing to unmask them from the Mycobacterium whole genus, in which, as minority members, they would have remained undetected
Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses
<p>Abstract</p> <p>Background</p> <p>Influenza virus binds to cell receptors via sialic acid (SA) linked glycoproteins. They recognize SA on host cells through their haemagglutinins (H). The distribution of SA on cell surfaces is one determinant of host tropism and understanding its expression on human cells and tissues is important for understanding influenza pathogenesis. The objective of this study therefore was to optimize the detection of α2,3-linked and α2,6-linked SA by lectin histochemistry by investigating the binding of Sambucus nigra agglutinin (SNA) for SAα2,6Gal and Maackia amurensis agglutinin (MAA) for SAα2,3Gal in the respiratory tract of normal adults and children.</p> <p>Methods</p> <p>We used fluorescent and biotinylated SNA and MAA from different suppliers on archived and prospectively collected biopsy and autopsy specimens from the nasopharynx, trachea, bronchus and lungs of fetuses, infants and adults. We compared different methods of unmasking for tissue sections to determine if these would affect lectin binding. Using serial sections we then compared the lectin binding of MAA from different suppliers.</p> <p>Results</p> <p>We found that unmasking using microwave treatment in citrate buffer produced increased lectin binding to the ciliated and glandular epithelium of the respiratory tract. In addition we found that there were differences in tissue distribution of the α2,3 linked SA when 2 different isoforms of MAA (MAA1 and MAA2) lectin were used. MAA1 had widespread binding throughout the upper and lower respiratory tract and showed more binding to the respiratory epithelium of children than in adults. By comparison, MAA2 binding was mainly restricted to the alveolar epithelial cells of the lung with weak binding to goblet cells. SNA binding was detected in bronchial and alveolar epithelial cells and binding of this lectin was stronger to the paediatric epithelium compared to adult epithelium. Furthermore, the MAA lectins from 2 suppliers (Roche and EY Labs) tended to only bind in a pattern similar to MAA1 (Vector Labs) and produced a different binding pattern to MAA2 from Vector Labs.</p> <p>Conclusion</p> <p>The lectin binding pattern of MAA may vary depending on the supplier and the different isoforms of MAA show a different tissue distribution in the respiratory tract. This finding is important if conclusions about the potential binding sites of SAα2,3 binding viruses, such as influenza or human parainfluenza are to be made.</p
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