Extended Infusion of Dexmedetomidine to an Infant at Sixty Times the Intended Rate

Dexmedetomidine is an α2 adrenergic agonist which has recently been approved in the United States for procedural sedation in adults. This report describes an infant who inadvertently received an intravenous infusion of dexmedetomidine at a rate which was 60 times greater than intended. We describe the hemodynamic, respiratory, and sedative effects of this overdose

Acquiring, Preserving, and Exhibiting a Comprehensive Collection of Vocal Music Recordings from Early- to Mid-Twentieth Century

The Stratton-Clarke collection consists of approximately 200 linear feet of 78 and 33 1/3 rpm records, and thousands of digitized recordings that represents a comprehensive history of early twentieth century recorded Western sound, specifically opera -- its artists, roles, and early legacy from 78 rpm to early long play records. Along with someephemera and several pieces of historic playback equipment, a large financial gift will offset the costs of processing, preserving and providing access to the various formats represented in the collection.  As the largest music research collection in Canada, the University of Toronto Music Library is fortunate to have the capacity to manage a donation of this magnitude. Each of our four authors has an important role to play to make the project a success.  In this article we present a history and background of John Stratton, Stephen Clarke, and the collection itself, and document the many facets of a library taking on a donation of this size: donor relations and collaboration with the University’s advancement team and other stakeholders; the project management involved in making space and designing workflow for cataloguing, processing, and storage; archival description of the 78s and ephemera; preservation of the digital objects and digitization strategies for the analog recordings; the challenges and opportunities of working with large financial gifts; teamwork and managing students; and future plans for physical and online exhibitions of the collection

Critical Role of FLRT1 Phosphorylation in the Interdependent Regulation of FLRT1 Function and FGF Receptor Signalling

Background Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. The mechanism by which the latter is achieved is still unknown and is the subject of this investigation. Principal Findings Here we show that FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). We identify the target tyrosine residues in the cytoplasmic domain of FLRT1 and show that these are not direct substrates for Src kinase suggesting that the SFK may exert effects via potentiation of FGFR1 kinase activity. We show that whilst FLRT1 expression results in a ligand-dependent elevation of MAP kinase activity, a mutant version of FLRT1, defective as an FGFR1 kinase substrate (Y3F-FLRT1), has the property of eliciting ligand-independent chronic activation of the MAP kinase pathway which is suppressed by pharmacological inhibition of either FGFR1 or Src kinase. Functional investigation of FGFR1 and FLRT1 signalling in SH-SY5Y neuroblastoma cells reveals that FLRT1 alone acts to induce a multi-polar phenotype whereas the combination of FLRT1 and FGFR activation, or expression of Y3F-FLRT1, acts to induce neurite outgrowth via MAPK activation. Similar results were obtained in a dendrite outgrowth assay in primary hippocampal neurons. We also show that FGFR1, FLRT1 and activated Src are co-localized and this complex is trafficked toward the soma of the cell. The presence of Y3F-FLRT1 rather than FLRT1 resulted in prolonged localization of this complex within the neuritic arbour. Conclusions This study shows that the phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an ‘in vivo’ role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth

Intensive Cultural Resources Survey Of 53 Acres Along Seber Road In Harris County, Texas

HRA Gray & Pape, LLC., of Houston, Texas performed an intensive archaeological pedestrian and reconnaissance-level walkover survey on an estimated 21.4-hectare (53-acre) property designated for residential development in Harris County, Texas. The project is being conducted on private property in anticipation of potential United States Army Corps of Engineers permitting requirements. All fieldwork and reporting activities were completed with reference to the Texas Antiquities Code 26.24, Council of Texas Archeologists guidelines, federal (National Historic Preservation Act) laws and guidelines (United States Department of the Interior 1981), and guidance for conducting cultural resources surveys pursuant to Section 106 of the National Historic Preservation Act (Advisory Council on Historic Preservation 2001). Site file research was completed using the online Texas Archaeological Sites Atlas maintained by the Texas Historical Commission. No previously recorded historic properties, historic markers, National Register of Historic Places, or archaeological sites were identified within a 1.6-kilometer (1-mile) radius during the background research. The review of the Texas Historical Commission files online did identify 2 areas that had been previously surveyed within the 1.6-kilometers (1-mile) search radius; however, these surveys did not identify archaeological resources. Field investigations were conducted on March 10, 2014 and required approximately 48 person hours to complete. All fieldwork and reporting activities were conducted and completed with reference to Section 106 of the National Historic Preservation Act, as amended and Texas survey Standards. The survey consisted of walkover, shovel testing, and photo-documentation of the permit area/Area of Potential Effects. Subsurface investigation included the excavation of 42 shovel tests, all of which were negative. During this investigation, no new or previously identified archaeological sites were recorded. Shovel testing identified soil profiles that gave no indication of buried cultural horizons. One extant historic-age storage barn was identified along the northwestern boundary of the property. The structure, constructed sometime after 1944, includes walls made of lumber and the roof was constructed of corrugated metal. None of the construction materials or methods appeared to be of unique design. Based on the negative results of this survey, HRA Gray & Pape, LLC. recommends no further cultural resources investigations within the property, and that the project be allowed to proceed as planned

Analysis of site structure and post-depositional disturbance at two Early Holocene components, Richard Beene site (41BX831), Bexar County, Texas

Two deeply buried, well-stratified, and well-dated components dating to the Early Holocene period were excavated at the Richard Beene site (41BX831) in Bexar County, Texas. This thesis utilizes both qualitative (interpretation of maps) and quantitative (unconstrained clustering) spatial analysis techniques to identify site structure and assess post-depositional disturbance by analyzing patterns among artifact categories, selected artifacts, and features from these components. Results of spatial analysis are compared to expectations of the archaeological record based on previous research. Each component revealed a distinct pattern. The Lower Medina component (ca. 6900 B.P.) is well preserved and spatial analysis showed clear distinctions between domestic and peripheral zones. The Upper Perez component (8800 B.P.) is a fluvial lag deposit of displaced artifacts and fire-cracked rock features. Results of spatial analysis confirmed that most, if not all, of this component is disturbed, revealing no site structure

Extended Infusion of Dexmedetomidine to an Infant at Sixty Times the Intended Rate

Dexmedetomidine is an α2 adrenergic agonist which has recently been approved in the United States for procedural sedation in adults. This report describes an infant who inadvertently received an intravenous infusion of dexmedetomidine at a rate which was 60 times greater than intended. We describe the hemodynamic, respiratory, and sedative effects of this overdose

MinXSS CubeSat On-Orbit Performance and the First Flight of the Blue Canyon Technologies XACT 3- axis ADCS

The Miniature X-ray Solar Spectrometer (MinXSS) 3U CubeSat was launched to the International Space Station (ISS) on 2015 December 6. Its deployment from the ISS is scheduled for 2016 March. MinXSS was designed and developed at the University of Colorado Boulder through a graduate project class, with significant professional support from the Laboratory for Atmospheric and Space Physics (LASP). The 3- axis attitude determination and control system (ADCS) is the Blue Canyon Technologies (BCT) XACT. This is the first flight of the XACT unit, which is the most capable commercially available 3-axis ADCS for CubeSats on the market today. MinXSS is a science mission funded by NASA\u27s Heliophysics division and is the first CubeSat to be flown from NASA Science Mission Directorate\u27s new CubeSat Implementation Panel. The primary objective for the MinXSS mission is to better understand the energy distribution of solar soft x-ray (SXR) emissions and their impact on earth\u27s ionosphere, thermosphere, and mesosphere (ITM). MinXSS observes the solar SXR spectrum between 0.5 to 30 keV with an energy resolution of 0.15 keV full width half maximum at 5.9 keV. Very few prior spectrally-resolved solar observations exist in the SXR range, leaving a critical gap in our ability to determine the spectral energy distribution for ITM modeling and solar flare studies. These issues can be addressed with new MinXSS data. This paper will provide details of the on-orbit performance of MinXSS and first-light observations from the primary science instrument, which is a commercially available system that was modified for flight. First light observations will include the first solar SXR spectrum from MinXSS and comparisons between quiet-Sun and flare spectra as observed by MinXSS. MinXSS represents the first opportunity for on-orbit characterization of BCT\u27s XACT ADCS. Performance of star tracker-based attitude determination, 3-axis reaction wheel-based attitude control, and torque rod-based momentum control will be assessed using on-orbit telemetry. This system is being used by several NASA centers, the DoD, many universities, and commercial entities for a multitude of upcoming missions that require precision attitude control at low cost. The exceptionally simple design of the LASP PPPT will be reviewed. The addition of a single fixed-value resistor mitigates the high current draw from the battery, which prevents the solar cell voltage from dropping below buck converter input requirements. The PPPT was successful in increasing the power output of the electrical power system by nearly a factor of 2 in mission simulations. The on-orbit power performance will be analyzed. In addition to thermal vacuum testing, MinXSS underwent thermal balance, which is dedicated to tuning the thermal model. The thermal balance procedure and model will be briefly overviewed and predictions compared to on-orbit temperatures. The results of this analysis have been generalized such that other CubeSat programs, who may not have the means to perform the test, may apply the results to their models and get improved model predictions. Thermal control of CubeSats is important to their lifetime and few if any prior results on this topic have been previously presented