Interdisciplinary cooperation between dentists and endocrinologists for identification and management of diabetes mellitus

Background: DM mellitus (DM) leads to worsening periodontal diseases, and in turn the inflammatory diseases of maxillofacial region adversely affect the glycemic control and exacerbate the severity of DM, thereby engendering a vicious cycle that compromises the DM management in patients. Taking account of the bidirectional relationship between DM and periodontal disease, interdisciplinary examination of patients with both DM and periodontal diseases is warranted to improve the health outcomes in patients. Aims: This study aims to evaluate the perceptions of dentists and endocrinologists on the interdisciplinary cooperation for identification and management of patients with DM. Materials and methods: Studying patients’ knowledge about DM and their compliance in providing endocrinological recommendations, dental screening survey to identify DM’ risk and signs The research was done in 2015-2016 years using clinical survey (dental status survey), statistical analysis. 432 patients from different dental organizations and 433 doctors (371 – dentists and 62 – endocrinologists) took part in the research. The research was approved by Regional research ethics committee. The written informed consent was taken from each participant. Results: There was insufficient interdisciplinary collaboration for identification and management of patients with diabetes, and lack of motivation among dental patients to endocrinological survey. Hence, it is important to incorporate definitive screening for risk of DM for patients with inflammatory periodontal disease and include dentists in consultation for patients with DM. The feasibility of statutory determination of collaboration between specialists in identification and management of patients with DM was found, dental lectures are necessary in DM school. Conclusions: Our findings suggest the necessity of including dentists in the standard of medical management of patients with DM and incorporating DM screening by a questionnaire upon dental examination

Supplementary data for the article: Radivojevic, J.; Skaro, S.; Senerovic, L.; Vasiljevic, B.; Guzik, M.; Kenny, S. T.; Maslak, V.; Nikodinovic-Runic, J.; O’Connor, K. E. Polyhydroxyalkanoate-Based 3-Hydroxyoctanoic Acid and Its Derivatives as a Platform of Bioactive Compounds. Applied Microbiology and Biotechnology 2016, 100 (1), 161–172. https://doi.org/10.1007/s00253-015-6984-4

Supplementary material for: [https://doi.org/10.1007/s00253-015-6984-4]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2025

Supplementary data for article: Narančić, T.; Kadivojevic, J.; Jovanovic, P.; Francuski, D.; Bigović, M.; Maslak, V.; Savić, V.; Vasiljević, B.; O’Connor, K. E.; Nikodinović-Runić, J. Highly Efficient Michael-Type Addition of Acetaldehyde to Beta-Nitrostyrenes by Whole Resting Cells of Escherichia Coli Expressing 4-Oxalocrotonate Tautomerase. Bioresource Technology 2013, 142, 462–468. https://doi.org/10.1016/j.biortech.2013.05.074

Supplementary material for: [https://doi.org/10.1016/j.biortech.2013.05.074]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1377

Overturning established chemoselectivities : selective reduction of arenes over malonates and cyanoacetates by photoactivated organic electron donors

The prevalence of metal-based reducing reagents, including metals, metal complexes, and metal salts, has produced an empirical order of reactivity that governs our approach to chemical synthesis. However, this reactivity may be influenced by stabilization of transition states, intermediates, and products through substrate-metal bonding. This article reports that in the absence of such stabilizing interactions, established chemoselectivities can be overthrown. Thus, photoactivation of the recently developed neutral organic superelectron donor 5 selectively reduces alkyl-substituted benzene rings in the presence of activated esters and nitriles, in direct contrast to metal-based reductions, opening a new perspective on reactivity. The altered outcomes arising from the organic electron donors are attributed to selective interactions between the neutral organic donors and the arene rings of the substrates

АКТИВНІСТЬ КАТЕПСИНІВ В, L, Н У ПЛАЗМІ КРОВІ ПАЦІЄНТІВ ІЗ ХРОНІЧНИМИ ДИФУЗНИМИ ЗАХВОРЮВАННЯМИ ПЕЧІНКИ

Introduction. Plasma markers are widely used along with trepanobiopsy to diagnose the histological stages of chronic diffuse liver diseases. The aim of the study – to determine the activity of cysteine cathepsins B, L, H and the content of proteolysis inhibitors α1-antitrinsin and α2-macroglobulin in the blood plasma of patients with chronic diffuse liver diseases of non-viral etiology. Research Methods. The object of research is the blood plasma of patients with chronic diffuse liver diseases (n=51) aged 28–60 years hospitalized in the Department of Liver and Pancreas Diseases of the Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine. The control group consisted of healthy volunteers (n = 15) aged from 25 to 52 years. The activity of cathepsins B, L and H were determined relatively to N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride, to azocasein and to oxytocin, respectively. The solution of N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride was used as a substrate for determination the content of inhibitors in human blood plasma. Results and Discussion. Compared with the group of practically healthy donors, statistically significant differences were recorded in: patients with steatohepatitis, the activity of cathepsin B increases by 26.7 %, and the level of α2-macroglobulin decreases by 30.7 %; in patients with chronic viral hepatitis with transition to cirrhosis, the activity of cathepsin B increases by 43.8 %, the activity level of cathepsin H decreases by 35 %, and the content of α2-macroglobulin, on the contrary, increases by 71.5 %; in the group of patients with steatohepatosis, the activity of cathepsin L and H decreases by 22.1 % and 25 %, respectively, and the concentration of α1-antitrypsin increases by 19.3 %. Conclusions. Determination of the content of inhibitors in conjunction with the activity of cysteine cathepsin in blood plasma can be proposed as non-invasive markers for chronic diffuse liver diseases of non-viral etiology.Вступление. Для диагностики гистологических стадий хронических диффузных заболеваний печени наряду с трепанобиопсией широко используют плазменные маркеры. Цель исследования – определить активность цистеиновых катепсинов В, L, H и содержание ингибиторов протеолиза α1-антитрипсина и α2-макроглобулина в плазме крови пациентов с хроническими диффузными заболеваниями печени невирусной этиологии и оценить значимость этих показателей для диагностики хронических заболеваний печени. Методы исследования. Объект исследования – плазма крови пациентов с хроническими диффузными заболеваниями печени (n=51) в возрасте 28–60 лет, которые находились на стационарном лечении в отделении заболеваний печени и поджелудочной железы Института гастроэнтерологии НАМН Украины. Контрольную группу составили здоровые волонтеры (n=15) в возрасте от 25 до 52 лет. Активность катепсинов В, L, Н определяли относительно N-α-бензоил-DL-аргинин-4-нитроанилида гидрохлорида, азоказеина и окситоцина соответственно. Для определения содержания ингибиторов в плазме крови в качестве субстрата использовали раствор N-α-бензоил-DL-аргинин-4-нитроанилида гидрохлорида. Результаты и обсуждение. По сравнению с группой практически здоровых доноров статистически значимые различия зафиксированы в таких группах: у пациентов с стеатогепатитом активность катепсина В возрастала на 26,7 %, а уровень α2-макроглобулина снижался на 30,7 %; у больных хроническим вирусным гепатитом с переходом в цирроз активность катепсина В повышалась на 43,8 %, активность катепсина H уменьшалась на 35,0 %, а содержание α2-макроглобулина, наоборот, увеличивалось на 71,5 %; у пациентов со стеатогепатозом активность катепсинов L и H снижалась на 22,1 и 25,0 % соответственно, а концентрация α1-антитрипсина возрастала на 19,30 %. Вывод. Определение уровня ингибиторов вместе с активностью цистеиновых катепсинов в плазме крови можно предложить в качестве неинвазивных маркеров при хронических диффузных заболеваниях печени невирусной этиологии.Вступ. Для діагностики гістологічних стадій хронічних дифузних захворювань печінки поряд із трепанобіопсією широко використовують плазмові маркери. Мета дослідження – визначити активність цистеїнових катепсинів В, L, H і вміст інгібіторів протеолізу α1-антитрипсину та α2-макроглобуліну в плазмі крові пацієнтів із хронічними дифузними захворюваннями печінки невірусної етіології. Методи дослідження. Об’єкт дослідження – плазма крові пацієнтів із хронічними дифузними захворюваннями печінки (n=51) віком 28–60 років, які перебували на стаціонарному лікуванні у відділенні захворювань печінки та підшлункової залози Інституту гастроентерології НАМН України. Контрольну групу становили здорові волонтери (n=15) віком від 25 до 52 років. Активність катепсинів В, L, Н визначали відносно N-α-бензоїл-DL-аргінін-4-нітроанілід гідрохлориду, азоказеїну, окситоцину відповідно. Для визначення вмісту інгібіторів у плазмі крові як субстрат використовували розчин N-α-бензоїл-DL-аргінін-4-нітроанілід гідрохлориду. Результати й обговорення. Порівняно з групою практично здорових донорів статистично значущі відмінності зафіксовано в таких групах: у пацієнтів із стеатогепатитом активність катепсину В зростала на 26,7 %, а рівень α2-макроглобуліну знижувався на 30,7 %; у хворих на хронічний вірусний гепатит з переходом у цироз активність катепсину В підвищувалась на 43,8 %, активність катепсину H зменшувалась на 35,0 %, а вміст α2-макроглобуліну, навпаки, збільшувався на 71,5 %; у пацієнтів із стеатогепатозом активність катепсинів L та H знижувалась на 22,1 і 25,0 % відповідно, а концентрація α1-антитрипсину зростала на 19,30 %. Висновок. Визначення рівня інгібіторів разом з активністю цистеїнових катепсинів у плазмі крові можна запропонувати як неінвазивні маркери при хронічних дифузних захворюваннях печінки невірусної етіології

Influence of suction on prolonged air leak after VATS lobectomies: a prospective randomized study

Background. Prolonged air leak is the most common postoperative complication following lung resection. Despite the huge number of researches concerning this problem, no consensus exists regarding the choice of the appropriate method of pleural space drainage after thoracoscopic surgery.Objective. To compare suction and water-seal regarding their influence on the incidence of prolonged air leak.Material and Methods. This prospective randomized trial included sixty patients who underwent VATS lobectomies on different diagnoses in the Center for Thoracic Surgery, Clinical Hospital no. 122 (St. Petersburg) from September 2018 until May 2020. The open-label randomized controlled trial involved two groups: control group (water-seal drainage) and suction group. Each group consisted of thirty patients. Ten patients were discharged with a Heimlich valve.Results. The incidence of prolonged air leak was 23%. Patients in the suction group had a higher duration of air leak than those in the control group (5.3 ± 1.3 vs 3.7 ± 0.9 days, р = 0.04). The number of air leak cases was slightly higher in the suction group (8 and 6 patients); however, the difference was not significant (р = 0.57). Both groups had no difference in the number of complications (р = 0.2). There were no cases of reoperation.Discussion. The advantage of water-seal is a lower risk of parenchymal defects. Suction may increase holes in visceral pleura, cause hyperexudation, leading to prolonged duration of drainage. At the same time, the use of suction may decrease residual pleural spaces, match visceral and parietal pleura, which may decrease the duration of air leak. A lot of studies on this issue was performed, however, their results are contradictory.Conclusion. Drainage of pleural space after VATS lobectomies may be safely performed with water-seal. In the case of increasing surgical emphysema or appearance of progressive dyspnea, suction should be applie

Analogue peptides for the immunotherapy of human acute myeloid leukemia

Accepted manuscript. The final publication is available at: http://link.springer.com/article/10.1007%2Fs00262-015-1762-9The use of peptide vaccines, enhanced by adjuvants, has shown some efficacy in clinical trials. However, responses are often short-lived and rarely induce notable memory responses. The reason is that self-antigens have already been presented to the immune system as the tumor develops, leading to tolerance or some degree of host tumor cell destruction. To try to break tolerance against self-antigens, one of the methods employed has been to modify peptides at the anchor residues to enhance their ability to bind major histocompatibility complex molecules, extending their exposure to the T-cell receptor. These modified or analogue peptides have been investigated as stimulators of the immune system in patients with different cancers with variable but sometimes notable success. In this review we describe the background and recent developments in the use of analogue peptides for the immunotherapy of acute myeloid leukemia describing knowledge useful for the application of analogue peptide treatments for other malignancies

Supplementary data for the article: Radivojevic, J.; Skaro, S.; Senerovic, L.; Vasiljevic, B.; Guzik, M.; Kenny, S. T.; Maslak, V.; Nikodinovic-Runic, J.; O’Connor, K. E. Polyhydroxyalkanoate-Based 3-Hydroxyoctanoic Acid and Its Derivatives as a Platform of Bioactive Compounds. Applied Microbiology and Biotechnology 2016, 100 (1), 161–172. https://doi.org/10.1007/s00253-015-6984-4

Supplementary material for: [https://doi.org/10.1007/s00253-015-6984-4]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2025