Efficacy of materials used by resource limited farmers in ethno-veterinary control of fleas in free-range chickens in the Eastern Cape Province, South Africa

Fleas are commonly controlled using commercial insecticides which are however expensive and inaccessible to resource-limited farmers. This has resulted in farmers resorting to the use of alternative remedies that are cheap and socially acceptable. However, information on the efficacy of these materials on fleas is lacking. The objectives of this study were to determine potential dermal irritation and efficacy of selected materials used in the control fleas in free-range chickens. The materials tested included used engine oil, Jeyes fluid (carbolic acid 13%), and plant material from Clutia pulchella and Calpurnia aurea. Distilled water and Karbadust (carbaryl 5%) were used as negative and positive controls, respectively. A complete randomized design was used where sixty free-range chickens were allocated to twelve treatment groups with five chickens each. The chickens were artificially infested with fleas, which were counted and recorded daily every morning for seven days. The results show that used engine oil, Jeyes fluid at (76.8%) and C. pulchella (100%) caused flea reduction in the order of 100, 99.8 and 85.5% three days post application of test materials. These reduction was similar to those of Karbadust (carbaryl 5%), a commercial insecticide that had a flea reduction of 100%. C. aurea (100%) and C. pulchella (75%) showed an efficacy of 75.5 and 60%, respectively. The different materials tested exhibited variable efficacy on fleas, where in certain cases it compared well with Karbadust used by farmers. The test materials did not cause any visible signs of irritation on chickens. Despite the efficacy of used engine oil and Jeyes fluid, they are environmental contaminants.Keywords: External parasites, flea control, skin irritation, used engine oilAfrican Journal of Biotechnology Vol. 12(14), pp. 1716-172

Investigation of the anti-inflammatory and antinociceptive activities of Elephantorrhiza elephantina (Burch.) Skeels root extract in male rats

The anti-inflammatory and anti-nociceptive activities of the root extract of Elephantorrhiza elephantina (Burch.) Skeels (Fabaceae) were investigated using wistar rats. The extract was administered intraperitoneally (i.p) to rats at graded doses of 50, 100 and 200 mg/kg BWt. Carrageenan andHistamine were injected into rat paws sub-plantar to induce paw oedema, while acetic acid and formalin were injected i.p to induce pain. Indomethacin (10 mg/kg) was used as reference drug, whereas thevehicle [0.9% normal saline in 3% tween 80 (2 ml/kg)] was used as negative control. Acute toxicity was tested in rats at doses of 200, 400, 800 and 1600 mg/kg BWt. Compared to control, the aqueous extractof E. elephantina at all doses investigated significantly (P < 0.05) reduced the formation of oedema induced by Carrageenan and Histamine. The extract also caused a significant (P < 0.05) reduction in writhings in the acetic acid test and licking time in the formalin test. The rats did not show any signs of acute toxicity. The study revealed the anti-inflammatory and anti-nociceptive activities of the aqueous extract of E. elephantina, which may be due to the presence of phytochemical constituents such astannins and flavonoids. The acute toxicity test showed that the plant is relatively safe to use

Contract farming approach to essential oil production in the Eastern Cape province of South Africa

Based on empirical evidence from small scale growers and a processor, this study evaluated contract farming of rose geranium (Pelagonium graveoleus) production in a rural setting of Eastern Cape Province of South Africa. With the aid of interview schedules and observation, cross sectional data were collected from the growers and processor of geranium oil on their 2007 production activities. The analytical tools employed descriptive statistics and farm budget model. The contract rose geranium growers in the area supplied the land for planting while the processor supplied the other inputs from land preparation to the harvesting of the plant. The contractual agreement was, however, unwritten with no signed contract between the parties. Further analysis revealed some ambiguities in the contractual arrangement. With an average of US $4 785.61 as production and processing costs on a hectare of rose geranium, the processor receives US$3 862.39 as net profit, while the contract growers received US $833.33. This arrangement appears unsustainable. Although contract farming is essentially private in the area, the arrangement can become an integral policy, where the government, together with the growers and agro-industry, join to create a conducive production environment. Sustainable contract farming of essential oil plants in South Africa, calls for a policy option that enforces well documented and clearly explained agreements between the farmers and the processors

Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves

Moringa (Moringa oleifera Lam. moringaceae) is a highly valued plant that is mostly cultivated in the tropics and subtropics. It is used for food, medication and industrial purposes. The objective of the study was to assess the nutritional value of Moringa leaves of the South African ecotype. Proximate and Van Soest methods were used to determine the nutritional value of Moringa leaves. The dried leaves had crude protein levels of 30.3% and 19 amino acids. The dried leaves had the following mineral contents: calcium (3.65%), phoshorus (0.3%), magnesium (0.5%), potassium (1.5%), sodium (0.164%), sulphur (0.63%), zinc (13.03 mg/kg), copper (8.25%), manganese (86.8 mg/kg), iron (490 mg/kg) and selenium (363 mg/kg). 17 fatty acids were observed with α-Linolenic acid (44.57%) having the highest value followed by heneicosanoic (14.41%), g-linolenic (0.20%) palmiteic (0.17%) and capric acid (0.07%). Vitamin E had the highest concentration of 77 mg/100 g than beta-carotene, which had 18.5 mg/100 g in the dried leaves. The fiber content was neutral detergent fibre (NDF) (11.4%), acid detergent  fibre (ADF) (8.49%), acid detergent lignin (ADL) (1.8%) and (acid detergent cellulose (ADC) (4.01%). The condensed tannins had a value of 3.2%, while total polyphenols were 2.02%. The values of amino acids, fatty acids, minerals and vitamin profiles reflect a desirable nutritional balance.Key words: South African, supplemental food, nutritional value, Moringa oleifera

Anti-inflammatory and analgesic activities of the aqueous extract of Leonotis leonurus leaves in rats

Leonotis leonurus (L.) R. BR. Lamiaceae is extensively for the treatment of various ailments and in the Eastern Cape province of South Africa, it is used for the treatment of effects of gastrointestinal parasites in animals. There is, however, scanty information on the pharmacological activities of this plant. The aqueous extract from the leaf of L. leonurus was investigated for its analgesic and antiinflammatory properties. Carrageenan and histamine-induced rat paw oedema were conducted to evaluate anti-inflammatory activity, while acetic acid-induced writhing test was conducted to assess the analgesic activity of the plant. The extract was administered intraperitoneally (i.p) to rats at graded doses of 50, 100, 200 mg/kg body weight (BWt). Indomethacin (10 mg/kg BWt) was used as reference drug, whereas the vehicle (2 mg/kg BWt of 0.9% normal saline in Tween-80) was used as negative control. Acute toxicity was tested in rats at doses of 200, 400, 800 and 1600 mg/kg BWt. Compared with the control, the plant extract at 100 and 200 mg/kg BWt significantly (P &lt; 0.05) reduced the formation of carrageenan - induced oedema, while with histamine-induced oedema the difference was insignificant (P &gt; 0.05). In the acetic acid-induced writhing model, the plant extract produced a significant (P &lt; 0.05) reduction in the number of writhes with all test doses and at 100 and 200 mg/kg BWt, the extract produced results that were similar to those of Indomethacin. This study revealed the potential of L. leonurus leaf aqueous extract in reducing pain and inflammation, suggesting that it has some antiinflammatory and analgesic activities, hence, justifying its ethno-veterinary uses. The acute toxicity test showed that the plant is relatively safe to use.Key words: Analgesic, anti-inflammation, indomethacin, Leonotis leonurus, rats

Evaluation of Xpert® MTB/RIF and ustar easyNAT™ TB IAD for diagnosis of tuberculous lymphadenitis of children in Tanzania : a prospective descriptive study

Fine needle aspiration biopsy has become a standard approach for diagnosis of peripheral tuberculous lymphadenitis. The aim of this study was to compare the performance of Xpert MTB/RIF and Ustar EasyNAT TB IAD nucleic acid amplification assays, against acid-fast bacilli microscopy, cytology and mycobacterial culture for the diagnosis of TB lymphadenitis in children from a TB-endemic setting in Tanzania.; Children of 8 weeks to 16 years of age, suspected of having TB lymphadenitis, were recruited at a district hospital in Tanzania. Fine needle aspirates of lymph nodes were analysed using acid-fast bacilli microscopy, liquid TB culture, cytology, Xpert MTB/RIF and EasyNAT. Latent class analysis and comparison against a composite reference standard comprising "culture and/or cytology" was done, to assess the performance of Xpert MTB/RIF and EasyNAT for the diagnosis of TB lymphadenitis.; Seventy-nine children were recruited; 4 were excluded from analysis. Against a composite reference standard of culture and/or cytology, Xpert MTB/RIF and EasyNAT had a sensitivity and specificity of 58 % and 93 %; and 19 % and 100 % respectively. Relative to latent class definitions, cytology had a sensitivity of 100 % and specificity of 94.7 %.; Combining clinical assessment, cytology and Xpert MTB/RIF may allow for a rapid and accurate diagnosis of childhood TB lymphadenitis. Larger diagnostic evaluation studies are recommended to validate these findings and on Xpert MTB/RIF to assess its use as a solitary initial test for TB lymphadenitis in children

Assessment of the medicinal potentials of the methanol extracts of the leaves and stems of Buddleja saligna

<p>Abstract</p> <p>Background</p> <p><it>Buddleja saligna </it>Willd (Loganiaceae) is a small to medium-sized evergreen tree; trunk short, often gnarled and crooked; crown dense, rounded or domed-shaped; foliage greyish green. The wild olives are traditionally used to lower blood pressures in many parts of the world. In southern Africa, bark and leaf decoctions are used to treat colic, coughs, colds, sore eyes, urinary problems and as purgatives.</p> <p>Methods</p> <p>The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of <it>Buddleja saligna </it>were evaluated using <it>in vitro </it>standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the leaves and stem extracts of <it>Buddleja saligna </it>were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant.</p> <p>Results</p> <p>The antioxidant activities of the leaves as determined by the ABTS and DPPH were similar to that of the stem. The flavonoids and the flavonols contents of the leaves were higher than that of the stem but the total phenols, proanthocyanidins and FRAP activities were higher in the methanol extracts of the stem. The extracts did show activity against both Gram-positive and Gram-negative bacteria. For instance, while the methanol extract of the leaves showed good activities on all the organisms except <it>Serratia marcescens </it>and <it>Pseudomonas aeruginosa </it>at MICs of between 2.5 and 5.0 mg/ml, the extract of the stem only showed activities on <it>Bacillus cereus, Streptococcus pyrogens </it>and <it>Pseudomonas aeruginosa </it>at the same concentration.</p> <p>Conclusion</p> <p>The results from this study indicate that the leaves and stem extracts of <it>Buddleja saligna </it>possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of <it>Buddleja saligna </it>are not as effective as the standard drugs-Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. <it>Buddleja saligna </it>may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products.</p

Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera

Abstract Background Acokanthera oppositifolia Lam (family: Apocynaceae) is a shrub or small tree with white latex, and the leaves of this plant are used in the form of a snuff to treat headaches and in infusions for abdominal pains and convulsions and septicaemia. Adenia gummifera Harv of the family Passifloraceae is a distinctive woody climber whose infusions are used as emetics and are said to help with some forms of depression. Lipid peroxidation has gained more importance today because of its involvement in pathogenesis of many diseases. Free radicals are the main agents in lipid peroxidation. Antioxidants thus play an important role of protecting the human body against damage by the free radicals. Plants containing phenolic compounds have been reported to possess strong antioxidant properties. Methods The antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera were evaluated using in vitro standard procedures. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Acokanthera oppositifolia were determined by the 2,2'-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-Diphenyl-2-picrylhydrazyl (DPPH), and ferrous reducing antioxidant property (FRAP) methods. Results The results from this study showed that the antioxidant activities of the stem extract of Acokanthera oppositifolia as determined by the 1,1-Diphenyl-2-picrylhydrazyl (DPPH), and ferrous reducing antioxidant property (FRAP) methods, were higher than that of Adenia gummifera. The levels of total phenols and flavonols for A. oppositifolia were also higher. On the other hand, the stem extract of Adenia gummifera had higher level of total flavonoids and proanthocyanidins than that of Acokanthera oppositifolia. The 2, 2'-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) activities of the 2 plant extracts were similar and comparable to that of BHT. Conclusion Thus, the present results indicate clearly that the extracts of Acokanthera oppositifolia and Adenia gummifera possess antioxidant properties and could serve as free radical inhibitors or scavengers, acting possibly as primary antioxidants. This study has to some extent validated the medicinal potential of the stems of Acokanthera oppositifolia and Adenia gummifera.</p

Toxicity evaluation of the aqueous leaf extract of Gunnera perpensa L. (Gunneraceae)

The objective of the study was to determine the potential toxicity of Gunnera perpensa through acute, sub-acute and chronic toxicity tests. For each test, 25 rats distributed in 5 groups of 5 rats per group were used. Group 1, the negative control, were orally administered with distilled water and groups 2 to 5 G. perpensa extract at 50, 100, 200 and 400 mg/kg doses, respectively. Change in behaviour and mortality was recorded. In addition, haematology, serum biochemical assays and histopathology examinations were conducted for sub-acute and chronic tests. Neither rat mortality nor changes in behaviour were noted for acute test. Rat mortality for 400 mg/kg dose of sub-acute and 200 mg/kg of chronic test was 20%. For sub-acute and chronic tests, platelets and monocytes were higher than the reference range, while mean cell volume was low. Creatinine, aspartate transaminase, magnesium, inorganic phosphate and potassium were low in the sub-acute while alkaline phosphatase and inorganic phosphate were low for the chronic test. Mild splenic siderosis and renal inflammation was observed in the sub-acute test. The plant is potentially toxic when used consecutively for a long period.Key words: Aqueous extract, Gunnera perpensa, haematology, mortality, serum biochemical parameters, toxicity