天然添加物に関わる基礎的調査研究 -その1 天然香料-

2006年厚労省により513種の天然香料其原物質がﾘｽﾄｱｯﾌﾟされた｡当ﾘｽﾄには動物､複合体､生成物､化合物､ｷﾉｺ類､海藻類､樹脂類､規制に関わる物質等が含まれるのでこれらは除いた450種の其原植物について調査研究を遂行することとした｡本ﾌﾟﾛｼﾞｪｸﾄでは天然香料の安全性確認のため以下の調査研究を行った｡1)天然香料に用いられる全ての原料の本体が明確になっていないので､最初に其原植物の学名を決定する｡2)安全性確認のためには食経験の調査が重要なので､食経験に関する文献を広い分野に求めそれらを記述し安全性の評価資料に資する｡3)近年分析機器の急速な発展により多くの成分が単離構造決定されているので､それぞれの其原植物に含まれる成分のﾃﾞｰﾀﾍﾞｰｽを作成する｡4)本ﾃﾞｰﾀﾍﾞｰｽが RTECS ﾃﾞｰﾀﾍﾞｰｽから得られる科学的安全性を支持することを確認する｡The natural resource of flavoring additives 513 items were listed in 2006. Since there are various kinds of complicated materials like animals, complexes, products, components, mushrooms, seaweeds, resins and materials related to the regulations, we removed such items in this project and relisted up 450 items. To make sure the safety of flavoring additives, the following have been investigated. 1) Since the principles of all original plant species using for flavoring additives have not been evident, we confirmed and determined their scientific names individually. 2) The eating experiences as food is important for the confirmation of safety. Therefore, the accumulated scientific data regarding eating experiences published in various fields were incorporated in this project resulting in evaluation of safety. 3) Recently so many natural compounds have been isolated and structurally elucidated depending on the development of purification and structural elucidation technique. We therefore prepared data base of components contained in individual flavoring additive. 4) It becomes evident that the above data base supported the scientific safety data of flavoring additives obtained from RTECS data

Identification and physiological evaluation of the components from Citrus fruits as potential drugs for anti-corpulence and anticancer

On the basis of monitoring the prevention of accumulation of lipid droplets in mouse 3T3-L1 preadipocyte cells and inhibition of the proliferation of human colon cancer HT-29 cells, effective anti-corpulence and anticancer compounds were isolated from the peel of Citrus fruits. These bioactive components were identified as polymethoxyflavones and coumarin derivatives by spectroscopic analyses. 5-Hydroxy-6,7,8,3′,4′-pentamethoxyflavone had the greatest anti-corpulence effects and 3,5,6,7,8,3′,5′-heptamethoxyflavone had the greatest anticancer effects. Furthermore, distributions of those bioactive components in the peel of 10 species of Citrus fruits were demonstrated by HPLC analyses