Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC-1

<p><b>Copyright information:</b></p><p>Taken from "Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC"</p><p>http://www.biomedcentral.com/1472-6750/7/84</p><p>BMC Biotechnology 2007;7():84-84.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2216013.</p><p></p> line 32-05-12 (filled squares) was cultured as a control. Viable cell density (A, solid lines), antibody concentration in the culture supernatant (A, dotted lines), and cell viability (B) were analyzed in the fed-batch culture. The oligosaccharide structures of the final products from 32-05-12 (C), FG1 (D), and FG16 (E) were analyzed using MALDI-TOF MS. The relative composition of each peak is shown as the relative amount to the total amount of oligosaccharide detected

Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC-0

<p><b>Copyright information:</b></p><p>Taken from "Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC"</p><p>http://www.biomedcentral.com/1472-6750/7/84</p><p>BMC Biotechnology 2007;7():84-84.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2216013.</p><p></p>n or hygromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human tRNApromoter (B or C). The siRNA tandem expression plasmid consisted of a hygromycin resistance gene and two short hairpin siRNA expression cassettes targeting and (D). The transcribed shRNAs and tRNA-shRNA fusion product were processed into siRNAs by Dicer

Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC-2

<p><b>Copyright information:</b></p><p>Taken from "Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC"</p><p>http://www.biomedcentral.com/1472-6750/7/84</p><p>BMC Biotechnology 2007;7():84-84.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2216013.</p><p></p>A). The antigen-binding activity of the antibody was measured by ELISA (B). Antibody purified from the serum-free fed-batch cultures of cells transformed by the and tandem siRNA expression vector, FG1 (open circles), FG16 (open squares), and the parental cell line 32-05-12 (filled circles) are shown. Cytotoxicity (%) and absorbance are indicated as the mean values ± SD of triplicates