Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC-0
<p><b>Copyright information:</b></p><p>Taken from "Double knockdown of α1,6-fucosyltransferase () and GDP-mannose 4,6-dehydratase () in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC"</p><p>http://www.biomedcentral.com/1472-6750/7/84</p><p>BMC Biotechnology 2007;7():84-84.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2216013.</p><p></p>n or hygromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human tRNApromoter (B or C). The siRNA tandem expression plasmid consisted of a hygromycin resistance gene and two short hairpin siRNA expression cassettes targeting and (D). The transcribed shRNAs and tRNA-shRNA fusion product were processed into siRNAs by Dicer
