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    Additional file 1: Figure S1. of Identification of resistant germplasm containing novel resistance genes at or tightly linked to the Pi2/9 locus conferring broad-spectrum resistance against rice blast

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    PCR amplification results of the 30 IRBLs and five rice cultivars using primer pair Pi2/9-DF1/DR1 (Pi2/9-RH). LTH, Lijiangxintuanheigu. IRBL, IRRI-bred blast-resistant lines. Figure S2. PCR amplification results of the 26 candidate resistant accessions using primer pair Pi2/9-DF1/DR1 (Pi2/9-RH). CO39 was used as a negative control while the Pi2 introgression line (IRBLz5-CA) was used as a positive control. Figure S3. Disease reaction of introgression line IR126183 (Pi2/9-A42) inoculated with the isolates 9244–3, M015–6 and 9482–1-3. R, resistance; PR, partial resistance; S, susceptibility; “+” indicates the PCR result is positive by Pi2/9-DF1/R1. Figure S4. Methods used for bioinformatics analysis of Pi2 and Nbs2-Pi2 alleles in 3K genomes. (PPTX 595 kb
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