Evaluation of biogenic amines in wine: Determination by an improved HPLC-PDA method.

An improved, simple and sensitive method to quantify the biogenic amines ethanolamine, methylamine, ethylamine, isoamylamine and the usually investigated b-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, spermine has been developed and validated in red and white wines. The analyses were derivatised with dansyl chloride and separated by HPLC coupled with PDA detector. The calibration curves showed good linearity (r>0.9990) and biogenic amines recovery varied from 72 to 97%. The repeatability ranged from 1 to 8% for red wine and from 1 to 5% for white wine. The detection and quantification limits were from 0.02 to 0.10 mg/L and from 0.08 to 0.30 mg/L, respectively. The method was successfully applied to detect and quantify biogenic amines in Italian red and white wines from Abruzzo Region. The proposed method is suitable for simultaneous detection and for accurate and precise quantification of eleven biogenic amines in wines

SACHER Project: A Cloud Platform and Integrated Services for Cultural Heritage and for Restoration

The SACHER project provides a distributed, open source and federated cloud platform able to support the life-cycle management of various kinds of data concerning tangible Cultural Heritage. The paper describes the SACHER platform and, in particular, among the various integrated service prototypes, the most important ones to support restoration processes and cultural asset management: (i) 3D Life Cycle Management for Cultural Heritage (SACHER 3D CH), based on 3D digital models of architecture and dedicated to the management of Cultural Heritage and to the storage of the numerous data generated by the team of professionals involved in the restoration process; (ii) Multidimensional Search Engine for Cultural Heritage (SACHER MuSE CH), an advanced multi-level search system designed to manage Heritage data from heterogeneous sources

Discovering Traffic Congestion along Routes of Interest using VANETs

One of the most interesting challenges for Intelligent Transportation System (ITS) consists of the traffic congestion problem. Congestion is a big obstacle for transportation since it reduces efficiency of the infrastructure and increases travel time, air pollution and fuel consumption. Nowadays, the most promising technology in support of ITS are Vehicular Ad Hoc Networks (VANETs). In this paper, we propose three protocols able to transmit traffic information for routes of interest on VANETs without any Road Side Unit (RSU) support. The protocols make a comparison between a reactive and a proactive approach and present strategies to improve the routing of the packets based on density and location of the vehicles. The objective is to keep high values of delivery ratio and accuracy using the smallest number of transmissions in order to guarantee scalability and to not saturate the bandwidth with only this type of packets. © 2013 IEEE

V2V protocols for traffic congestion discovery along routes of interest in VANETs: a quantitative study

One of the most interesting and promising challenges for Intelligent Transportation Systems (ITSs) relates to the traffic congestion problem. Congestion is a relevant issue for transportation because it reduces the efficiency of infrastructure and increases travel time, air pollution, and fuel consumption. Nowadays, the most promising technology in support of ITSs is found in the domain of Vehicular Ad Hoc Networks (VANETs). In this paper, we propose three protocols that are able to transmit traffic information for routes of interest on VANETs without any Road Side Unit (RSU) support. The proposed protocols adopt strategies to improve the performance of packet routing based on the density and location of vehicles; moreover, they enable an interesting comparison of the performance achievable with either reactive or proactive approaches. The extensive performance results reported show how it is possible to limit the congestion monitoring overhead along Routes of Interest (ROIs), while maintaining a sufficiently high performance in terms of traffic reporting. This may be done by employing context-aware data delivery techniques that autonomously adapt to runtime conditions. Copyright © 2016 John Wiley & Sons, Ltd

Biogenic amines contenta as a measure of the quality of wines of Abruzzo (Italy).

Aim of this research was to study the effect of some agronomic and oenological factors on the content of biogenic amines as quality index of wines from 14 wineries of the Abruzzo (Italy). Out of 66 samples of wine (bottled and ready to trade) the following amines have been researched, identified and quantified: ethylamine (ETY), 2-phenylethylamine (2-PHE), putrescine (PUT), cadaverine (CAD), isoamylamine (ISO), histamine (HIS), tyramine (TYR), spermidine (SPD) and spermine (SPM).Sum of amines was found to be decreasing in the order: red wine (19.3 ± 12.8 mg L-1), rose wine (9.20 ± 6.34 mg L-1), white wine (7.67 ± 3.84 mg L-1). The single amines significantly correlated with their sum were the PUT (β = 0.94), the HIS (β = 0.91) and TYR (β = 0.89). The production of PUT was significantly associated (β = 0.69) with activity of malolactic bacteria (Mangani et al., 2005). The principal component analysis (PCA), as a result of autoscaling the data, explained about 50% of the total variability of the samples. The first component (LV1) was positively associated with PUT, HIS, TYR, and pH, negatively with malic acid content, discriminating red wines from white ones significantly. The second component (LV2) was negatively associated with ethanol and positively with CAD and total SO2 amount, discriminating white wines from the rest of the samples.The differences among the types of wine are due to different biotechnological process: microbial strains involved in the fermentation of white winemaking, have characteristics different from those involved in the red one, the environmental conditions that are created during the red winemaking favor an increased availability of substrates and optimal parameters (pH, T, etc. ..) for the amino acid decarboxylase activity. Besides the vintage, influential seems to be the effect of the winery, regardless of the geographic area in which it is situated. However, in all analyzed samples the maximum amounts of TYR and HIS were below the levels of toxicity (Konakovsky et al., 2011), demonstrating a good quality of the wines of Abruzzo, whose consumption is no risk to the health of the consumer following the rules of proper nutrition. Authors are very grateful for the co-operation of the Association of Enologists of Abruzzo-Molise.[...

Usaturated polyester resins: a study on mechanism and kinetics of the curing process by FTiR spectroscopy

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