COMPARISON OF PEAK LUMBAR LORDOSIS BETWEEN SOME BASIC MOVEMENTS OF RHYTHMIC GYMNASTICS

To assist coaches in managing gymnasts, a good knowledge of the movements most likely to cause back pain is required. In rhythmic gymnastics (RG), this can be considered as identifying movements involving very high lumbar curvatures. To quantify the lumbar lordosis during some basic RG movements (ring, penché, penché with rotation, split leap, turning split leap, and front and back walkovers), eight gymnasts were enrolled and a 3D motion analysis was performed based on motion capture data, a musculoskeletal model and low-dose biplanar radiographs for model personalisation purposes. The ring and both the front and back walkovers were the movements studied involving the lumbar spine in extension the most but also resulting in the highest dispersion between gymnasts. Hence, future works should investigate the causes of this greater dispersion

Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation

AbstractThe tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and CD151, were found to incorporate [3H]palmitate. By site-directed mutagenesis, CD9 was found to be palmitoylated at any of the four internal juxtamembrane regions. The palmitoylation of CD9 did not influence the partition in detergent-resistant membranes but contributed to the interaction with CD81 and CD53. In particular, the resistance of the CD9/CD81 interaction to EDTA, which disrupts other tetraspanin/tetraspanin interactions, was entirely dependent on palmitoylation

Dosimetric planning of radioiodine therapy on the basis of pharmacokinetic modeling

The program complex of pharmacokinetic modeling and dosimetric planning of radioiodine therapy on the basis of clinical diagnostic data is developed. For 16 patients with the diagnosis «diffuse toxic goiter» (Graves' disease) individual kinetic parameters of transport of the thyroid radiopharmaceutical taken orally are identified and calculations of the absorbed doses in the thyroid, the stomach, the blood tissue, and the periodic-depletion bladder are performed. Three approaches to purpose of activity of radiopharmaceutical and feature of individual dosimetric planning of radioiodine therapy are considered and analysed

Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker.

CD63, a member of the tetraspanin superfamily, is used as a marker of late endosomes and lysosome-related organelles, as well as a marker of exosomes. Here, we selected rare isotype variants of TS63 by sorting hybridoma cells on the basis of their high expression of surface immunoglobulins of the IgG2a and IgG2b subclass. Pure populations of cells secreting IgG2a and IgG2b variants of TS63 (referred to as TS63a and TS63b) were obtained using two rounds of cell sorting and one limited dilution cloning step. We validate that these new TS63 variants are suitable for co-labeling with mAb of the IgG1 subclass directed to other molecules, using anti mouse subclass antibodies, and for the labeling of exosomes through direct binding to protein A-coated gold particles. These mAbs will be useful to study the intracellular localization of various proteins and facilitate electron microscopy analysis of CD63 localization

Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p 24) Inhibition of aggregation by ALB6Fab

AbstractThe leukemia-associated cell surface antigen p 24 is found on normal platelets as well as on Bernard Soulier syndrome and thrombasthenia type I platelets. ALB6 IgG (a monoclonal antibody against p 24) induces the aggregation of platelets from normal donors but not from thrombasthenia. In contrast, ALB6 Fab inhibits platelet aggregation induced by collagen, ADP, thrombin, ionophore A 23187 and ALB6 IgG. The results suggest that ALB6 interferes with a mechanism common to all aggregation pathways; the possible mechanisms are discussed

Un site protohistorique littoral dans le havre de la Vanlée à Lingreville et Bricqueville-sur-Mer (Manche)

The increasing erosion of the coastline in the «havre de la Vanlée» (Manche) has led to most archeologicals discoveries. The site of Lingreville III has in particular provided an abundant quantity of protohistoric domestic material falled in the filling of a paleochannel. The site allows environnemental studies and an approach of the coastlines variations at the end of the Holocene. The abundant ceramic ware is heterogeneous and seems composed, on one hand, of a Bell Beaker-Early Bronze Age assemblage and, on the other hand, of an assemblage of the Late Bronze Age for the major part.L'érosion croissante du front de mer dans le havre de la Vanlée (Manche) a donné lieu à plusieurs découvertes de sites archéologiques sur estran. Différents gisements (Lingreville II et III, Bricqueville-sur-Mer) ont en particulier livré une grande quantité de mobilier protohistorique piégé dans le colmatage d'un paléochenal. Le site autorise des études environnementales ainsi qu'une approche des variations de la ligne de rivage à la fin de l'Holocène. L'abondant mobilier céramique appartient à une série hétérogène qui semble composée pour l'essentiel, d'une part, d'un ensemble Campaniforme-Bronze ancien et d'autre part, d'un ensemble dont la datation est ici discutée et qui appartient au Bronze final.Billard Cyrille, Clet-Pellerin Martine, Lautridou Jean-Pierre, Giffault Michèle. Un site protohistorique littoral dans le havre de la Vanlée à Lingreville et Bricqueville-sur-Mer (Manche). In: Revue archéologique de l'ouest, tome 12, 1995. pp. 73-110

CD46 (membrane cofactor protein) associates with multiple β1 integrins and tetraspans

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New insights into the tetraspanin Tspan5 using novel monoclonal antibodies

International audienceTspan5 is a member of a subgroup of tetraspanins referred to as TspanC8. These tetraspanins directly interact with the metalloprotease ADAM10, regulate its exit from the endoplasmic reticulum and subsequent trafficking, and differentially regulate its ability to cleave various substrates and activate Notch signaling. The study of Tspan5 has been limited by the lack of good antibodies. This study provides new insights into Tspan5 using new monoclonal antibodies (mAbs), including two mAbs recognizing both Tspan5 and the highly similar tetraspanin Tspan17. Using these mAbs, we show that endogenous Tspan5 associates with ADAM10 in human cell lines and in mouse tissues where it is the most abundant, such as the brain, the lung, the kidney, or the intestine. We also uncover two TspanC8-specific motifs in the large extracellular domain of Tspan5 that are important for ADAM10 interaction and exit from the endoplasmic reticulum. One of the anti-Tspan5 mAbs does not recognize Tspan5 associated with ADAM10, providing a convenient way to measure the fraction of Tspan5 not associated with ADAM10. This fraction is minor in the cell lines tested, and it increases upon transfection of cells with TspanC8 tetraspanins such as Tspan15 or Tspan33 that inhibit Notch signaling. Finally, two antibodies inhibit ligand-induced Notch signaling, and this effect is stronger in cells depleted of the TspanC8 tetraspanin Tspan14, further indicating that Tspan5 and Tspan14 can compensate for each other in Notch signaling

Effect of Iterative Reconstruction on the Detection of Systemic Sclerosis-related Interstitial Lung Disease: Clinical Experience in 55 Patients

International audiencePurposeTo evaluate the effect of iterative reconstruction on the depiction of systemic sclerosis–related interstitial lung disease (ILD) when the radiation dose is reduced by 60%.Materials and MethodsThis study was based on retrospective interpretation of prospectively acquired data over a 12-month period and approved by the institutional review board. The requirement to obtain informed consent was waived. Fifty-five chest computed tomographic (CT) examinations were performed in 38 women and 17 men (mean age, 55.8 years; range, 23–82 years) by using a dual-source CT unit with (a) both tubes set at similar energy (120 kVp) and (b) the total reference milliampere seconds (ie, 110 mAs) split up in a way that 40% was applied to tube A and 60% to tube B. Two series of images were generated simultaneously from the same dataset: (a) standard-dose images (generated from both tubes) reconstructed with filtered back projection (group 1, the reference standard) and (b) reduced-dose images (generated from tube A; 60% dose reduction) reconstructed with sinogram-affirmed iterative reconstruction (SAFIRE) (group 2). In both groups, the analyzed parameters comprised the image noise and the visualization and conspicuity of CT features of ILD. Two readers independently analyzed images from both groups. Results were compared by using the Wilcoxon test for paired samples; the 95% confidence interval was calculated when appropriate.ResultsThe mean level of objective noise in group 2 was significantly lower than that in group 1 (22.02 HU vs 26.23 HU, respectively; P < .0001). The CT features of ILD in group 1 were always depicted in group 2, with subjective conspicuity scores (a) improved in group 2 for ground-glass opacity, reticulation, and bronchiectasis and/or bronchiolectasis and (b) identical in both groups for honeycombing. The interobserver agreement for their depiction was excellent in both groups (κ, 0.84–0.98).ConclusionDespite a 60% dose reduction, images reconstructed with SAFIRE allowed similar detection of systematic sclerosis–related ILD compared with the reference standard