2 research outputs found
<i>In Vivo</i> Fate of Avidin-Nucleic Acid Nanoassemblies as Multifunctional Diagnostic Tools
This study describes the formulation optimization and body-cell distribution and clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on the novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as a potential advancement of classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely in the bloodstream; it is slowly captured by filter organs; it is efficiently cleared within 24ā48 h, and it is poorly immunogenic. The system displays more favorable properties than its parent monomeric avidin and it is a promising tool for diagnostic purposes for future translational aims, for which free circulation in the bloodstream, safety, multifunctionality and high composition definition are all necessary requirements. In addition, the assembly shows a time-dependent cell penetration capability, suggesting it may also function as a NP-dependent drug delivery tool. The ease of preparation together with the possibility to fine-tune the surface composition makes it also an ideal candidate to understand if and how nanoparticle composition affects its localization
Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release
One
of the main hurdles in nanomedicine is the low stability of
drugānanocarrier complexes as well as the drug delivery efficiency
in the region-of-interest. Here, we describe the use of the film-forming
protein hydrophobin HFBII to organize dodecanethiol-protected gold
nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained
SPs are exceptionally stable <i>in vivo</i> and efficiently
encapsulate hydrophobic drug molecules. The HFBII film prevents massive
release of the encapsulated drug, which, instead, is activated by
selective SP disassembly triggered intracellularly by glutathione
reduction of the protein film. As a consequence, the therapeutic efficiency
of an encapsulated anticancer drug is highly enhanced (2 orders of
magnitude decrease in IC<sub>50</sub>). Biodistribution and pharmacokinetics
studies demonstrate the high stability of the loaded SPs in the bloodstream
and the selective release of the payloads once taken up in the tissues.
Overall, our results provide a rationale for the development of bioreducible
and multifunctional nanomedicines