Multi-generational House

BUTOROVÁ, H.: Dvougenerační rodinný dům: Bakalářská práce. Ostrava: VŠB-Technická univerzita Ostrava, Fakulta stavební, Katedra architektury 226, 2017, 49 s. Vedoucí práce: Student, A. Předmětem bakalářské práce „Dvougenerační rodinný dům“ je vypracování částečné projektové dokumentace pro provádění stavby podle vyhlášky 499/2006 Sb., o dokumentaci staveb. Jako podklad bakalářské práce slouží architektonická studie vypracovaná v rámci předmětu Ateliérová tvorba I a dokumentace pro stavební povolení vypracovaná v předmětu Ateliérová tvorba Va. Rodinný dvougenerační dům je navržen v lázeňské oblasti Karviná-Darkov. Stavba je složena z části pro mladou rodinu a z části pro starší rodiče. Cílem bylo vytvořit společné zázemí obou rodin, avšak i dostatek soukromí. Koncepce domu je založena na přízemní části staršího páru a na dvoupodlažní části mladé čtyřčlenné rodiny.BUTOROVÁ, H.: Multi-generational House: Bachelor´s thesis. Ostrava: VŠB-Technical university of Ostrava, Faculty of Civil Engineering, Department of Architecture 226, 2017, 49 p. Thesis head: Student, A. The subject of bachelor’s thesis „Multi-generational House‟ is preparation of partial project documentation for construction of a building according to notice 499/2006 Sb., about documentation of buildings. As resource materials serves architectural study worked out from Studio Work I and a documentation for building permit worked out from Studio Work Va. Multi-generational House is projected in the spa area Karviná-Darkov. The building consists of a part for young family and a part for grandparents. The goal was to make a common base for both families, but also to secure enough privacy. The philosophy of the house is based on the ground part for older couple and on the two-floor part for young four-member family.226 - Katedra architekturyvelmi dobř

Effects of 1,25(OH)₂D₃ and 25(OH)D₃ on VDR, CYP27B1 and CYP24 mRNA levels in primary human osteoblast.

<p>Osteoblasts were cultured in the presence of 0, 1, 10 and 100 nM 1,25(OH)₂D₃ or 0, 100, 200 and 400 nM 25(OH)D₃ for 24 hours and mRNA levels of VDR (<b>A</b>), CYP27B1 (<b>B</b>) and CYP24 (<b>C</b>) were determined. Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line) using cells from 5 or 6 different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test (*p<0.05, **p<0.01, ***p<0.001).</p

Supplementary file for: “Changes in serum testosterone and adrenal androgen levels in transgender women with and without gonadectomy.”

Supplementary file to Collet et al. 2022: Changes in serum testosterone and adrenal androgen levels in transgender women with and without gonadectomy. This supplemenary file contains:  1) Method comparison between the endocrine laboratories of the UZ Gent and Amsterdam UMC for total testosterone, androstenedione and SHBG.  2) Supplementary analyses</p

Effects of 24R,25(OH)₂D₃ on VDR, CYP27B1 and CYP24 mRNA levels in primary human osteoblasts.

<p>Osteoblasts were cultured in the presence of 0, 100, 200 or 400 nM 24R,25(OH)₂D₃ and mRNA levels of VDR (<b>A</b>), CYP27B1 (<b>B</b>) and CYP24 (<b>C</b>) were determined after 72 hours. Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line) using cells from 4 different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test (*p<0.05, **p<0.01, ***p<0.001).</p

Effects of 24R,25(OH)₂D₃ on mRNA levels of genes involved in osteoblast differentiation.

<p>Osteoblasts were cultured in the presence of 0, 100, 200 or 400 nM 24R,25(OH)₂D₃ and mRNA levels of COL1α1 (<b>A</b>), ALP (<b>B</b>), osteocalcin (<b>C</b>) and osteopontin (<b>D</b>) were determined after 72 hours. Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line) using cells from 4 different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test (*p<0.05, **p<0.01, ***p<0.001).</p

Effects of 1,25(OH)₂D₃ and 25(OH)D₃ on mRNA levels of genes involved in primary human osteoblast differentiation.

<p>Osteoblasts were cultured in the presence of 0, 1, 10 and 100 nM 1,25(OH)₂D₃ or 0, 100, 200 or 400 nM 25(OH)D₃ for 10 days in osteogenic medium and mRNA levels of ALP (<b>A</b>), COL1α1 (<b>B</b>), osteocalcin (<b>C</b>) and osteopontin (<b>D</b>) were determined. Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line) using cells from 5 different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test (*p<0.05, **p<0.01, ***p<0.001).</p

Effects of 1,25(OH)₂D₃ and 25(OH)D₃ on primary human osteoblast proliferation.

<p>Osteoblasts were cultured in the presence of 0, 1, 10 or 100 nM 1,25(OH)₂D₃ (<b>A</b>) and 0, 100, 200 or 400 nM 25(OH)D₃ (<b>B</b>) and the proliferation was quantified at day 3 and 6. Results (mean ± SEM) are expressed as treatment versus control ratios (time-point 0 was set at 1.0) using cells from 4 (A) or 7 (B) different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test for each timepoint (*p<0.05, **p<0.01, ***p<0.001).</p

Synthesis of 1,25(OH)₂D₃ and 24R,25(OH)₂D₃ by primary human osteoblasts.

<p>Osteoblasts were cultured in the presence of 0, 100, 200, 400 and 1.000 nM 25(OH)D₃ for 24 hours and 25(OH)D₃ (<b>A</b>) 1,25(OH)₂D₃ (<b>B</b>) and 24R,25(OH)₂D₃ (<b>C</b>) levels were measured in non-conditioned and conditioned culture medium. Results are expressed as mean ± SEM using cells from 3 different donors.</p

Bone formation markers in PHI-men, * = significant difference p ≤ 0.05 of follow-up versus baseline, ● = cART treated group, ♦ = untreated group, B = baseline, median and IQR, FU = follow-up, median and IQR.

<p>Bone formation markers in PHI-men, * = significant difference p ≤ 0.05 of follow-up versus baseline, ● = cART treated group, ♦ = untreated group, B = baseline, median and IQR, FU = follow-up, median and IQR.</p

Effects of 1,25(OH)₂D₃ and 25(OH)D₃ on ALP activity, P1NP and osteocalcin secretion by primary human osteoblast.

<p>Osteoblasts were cultured in the presence of 0 or 100 nM 1,25(OH)₂D₃ and 0 or 400 nM 25(OH)D₃ and ALP activity (<b>A</b> and <b>B</b> respectively), P1NP (<b>C</b> and <b>D</b> respectively) and osteocalcin secretion (<b>E</b> and <b>F</b> respectively) were measured at day 3, 7, 10 and 14 of the differentiation. Results are expressed as mean ± SEM using cells from 5 different donors. Results were analyzed using Wilcoxon signed rank test for each timepoint (*p<0.05, **p<0.01, ***p<0.001).</p