3 research outputs found
Fast Diffusion-Limited Lyotropic Phase Transitions Studied in Situ Using Continuous Flow Microfluidics/Microfocus-SAXS
Fast concentration-induced diffusion-limited
lyotropic phase transitions
can be studied in situ with millisecond time resolution using continuous
flow microfluidics in combination with microfocus small-angle X-ray
scattering. The method was applied to follow a classical self-assembly
sequence where amphiphiles assemble into micelles, which subsequently
assemble into an ordered lattice via a disorder/order transition.
As a model system we selected the self-assembly of an amphiphilic
block copolymer induced by the addition of a nonsolvent. Using microchannel
hydrodynamic flow-focusing, large concentration gradients can be generated,
leading to a deep quench from the miscible to the microphase-separated
state. Within milliseconds the block copolymers assembly via a spinodal
microphase separation into micelles, followed by a disorder/order
transition into an FCC liquid-crystalline phase with late-stage domain
growth and shear-induced domain orientation into a mesocrystal. A
comparison with a slow macroscopic near-equilibrium kinetic experiment
shows that the fast structural transitions follow a direct pathway
to the equilibrium structure without the trapping of metastable states
Low-Loading Mixed Matrix Materials: Fractal-Like Structure and Peculiarly Enhanced Gas Permeability
Mixed matrix materials (MMMs) containing
metal–organic framework
(MOF) nanoparticles are attractive for membrane carbon capture. Particularly,
adding <5 mass % MOFs in polymers dramatically increased gas permeability,
far surpassing the Maxwell model’s prediction. However, no
sound mechanisms have been offered to explain this unusual low-loading
phenomenon. Herein, we design an ideal series of MMMs containing polyethers
(one of the leading polymers for CO2/N2 separation)
and discrete metal–organic polyhedra (MOPs) with cage sizes
of 2–5 nm. Adding 3 mass % MOP-3 in a polyether increases the
CO2 permeability by 100% from 510 to 1000 Barrer at 35
°C because of the increased gas diffusivity. No discernible changes
in typical physical properties governing gas transport properties
are detected, such as glass transition temperature, fractional free
volume, d-spacing, etc. We hypothesize that this
behavior is attributed to fractal-like networks formed by highly porous
MOPs, and for the first time, we validate this hypothesis using small-angle
X-ray scattering analysis
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX