Introducing MARCo: Histoserological Findings of a Multi-Organic Paraneoplastic Syndrome in Cutaneous Melanoma Patients

<p><b>Article full text</b></p> <p><br></p> <p>The full text of this article can be found here<b>. </b><a href="https://link.springer.com/article/10.1007/s13555-016-0142-5">https://link.springer.com/article/10.1007/s13555-016-0142-5</a></p> <p><br></p> <p><b>Provide enhanced content for this article</b></p> <p><br></p> <p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p> <p><br></p> <p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p> <p><br></p> <p>Other enhanced features include, but are not limited to:</p> <p><br></p> <p>• Slide decks</p> <p>• Videos and animations</p> <p>• Audio abstracts</p> <p>• Audio slides</p

Basta!: Für Saxophonquartett

<div><p>Mosquitoes and other arthropods may transmit medically important pathogens, in particular viruses such as West Nile virus. The presence of suitable hosts and competent vectors for those zoonotic viruses is essential for an enzootic transmission, which is a prerequisite for epidemics. To establish reliable risk projections, it is an urgent need for an exact identification of mosquito species, which is especially challenging in the case of sibling species, such as <i>Culex. pipiens pipiens</i> biotypes <i>pipiens</i> and <i>molestus</i>. To facilitate detection of different <i>Culex pipiens</i> forms and their hybrids we established a multiplex real-time PCR. <i>Culex pipiens</i> samples were obtained by egg raft collection and rearing until imago stage or adult sampling using CO₂ baited traps and gravid traps. In total, we tested more than 16,500 samples collected all over Germany in the years 2011 and 2012. The predominant species in Germany are <i>Culex pipiens pipiens</i> biotype <i>pipiens</i> and <i>Culex. torrentium</i>, but we also detected <i>Culex pipiens pipiens</i> biotype <i>molestus</i> and hybrids of the two <i>pipiens</i> biotypes at sites where both species occur sympatrically. This report of a potentially important bridge vector for West Nile virus might have major impact in the risk projections for West Nile virus in Germany.</p></div

Classification of <i>Culex</i> samples from the German mosquito surveillance program.

<p>Graphical representation of the Culex species composition in Germany. 48 different trapping sites in Germany were combined according to their geographical relatedness to form 10 cluster areas shown representing Lower Rhine Valley and further sites in Palatine, Upper Rhine Valley and further sites in Baden-Württemberg, Lake Constance, Lake Chiemsee and other sites in Bavaria, Hesse, Upper Elbe Valley in Saxonia, Oder Valley in Brandenburg, Baltic Sea in Mecklenburg-West Pomerania, Metropolitan Region Hamburg and various sites in Schleswig-Holstein (for more details see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071832#pone-0071832-t001" target="_blank">Table 1</a>)). White (<i>Cpp. pipiens</i>), black (<i>Cx. torrentium</i>) and red (<i>Cpp. molestus</i>) quarters indicate pools that were composed of a single species. Grey (<i>Cpp. pipiens</i>+<i>Cx. torrentium</i>) and dark-red (<i>Cpp. molestus</i>+<i>Cx. torrentium</i>) quarters indicate pools composed of two species. With the current set-up (i.e.using pooled samples) the composition of pink quarters could be either two biotypes <i>Cpp. pipiens</i> and <i>Cpp. molestus</i> or hybrids of both biotypes. The n-numbers given in the graphs notify total numbers of individuals analysed in each cluster.</p

Identification of <i>Cpp.</i> biotype hybrids in two German metropolitan areas.

<p>Detailed species composition at the 4 sampling sites of the Rhine-Main metropolitan area (right graph) and 5 sampling sites at Hamburg metropolitan area (left graph). DNA samples from single individuals collected at these sampling sites were subjected to the multiplex real-time PCR and analysed for the presence of biotype hybrids. Bars represent species distribution in percent at each site. White indicates <i>Cpp.</i> biotype <i>pipiens</i>; red <i>Cpp.</i> biotype <i>molestus</i>; yellow hybrids of biotypes <i>pipiens</i> and <i>molestus</i>. Biotype hybrids were found at Dirmstein and Bobenheim-Roxheim trapping sites in Southwest Germany and at the Wulksfelde trapping site in northern Germany. Additionally, the presence of <i>Cx. torrentium</i> was assed and is indicated by the grey bars in both graphs.</p

Trapping-sites of the nationwide surveillance program selected for the study.

<p>Trap types: BT: Biogents Sentinel; GT: Gravid trap; EVS: Enceph. Vector Surveillance Trap (Bioquip); ex-La.: reared from larvae ; ex-Pu.: reared from pupae.</p

JIKI trial: participants’ characteristics, according to age and baseline Ct value.

<p>JIKI trial: participants’ characteristics, according to age and baseline Ct value.</p

JIKI trial: correlation between EBOV RT-PCR Ct value and RNA viral load at baseline in adolescents and adults.

<p>The black line represents the regression line. The Spearman rank correlation coefficient was 0.88 (<i>p</i> < 0.001). A baseline Ct value of 20 corresponded to an RNA viral load of 5 × 10⁷ genome copies/ml of plasma (7.7 log₁₀ copies/ml). Of the 99 adolescents and adults, 84 had both an RT-PCR Ct and an RNA viral load measurement at baseline. Red dots represent patients who died. Blue dots represent patients who survived.</p

JIKI trial: evolution of serum creatinine, aspartate aminotransferase, alanine aminotransferase, and creatine phosphokinase in adolescents and adults.

<p>The <i>x</i>-axis represents the time since first symptoms (for example, for a patient whose first symptom occurred 5 d before day 0, the baseline value dot is positioned at 5 d). Each line represents one patient. Dots represent baseline values, and X’s represent follow-up values. Red symbols represent patients who died; blue symbols represent patients who survived. Dark red lines represent patients with baseline Ct < 20 who died, light red lines represent patients with baseline Ct ≥ 20 who died, dark blue lines represent patients with baseline Ct < 20 who survived, and light blue lines represent patients with baseline Ct ≥ 20 who survived. Samples obtained more than 25 d after onset of symptoms are not represented.</p

JIKI trial: evolution of serum creatinine, transaminases, and creatine phosphokinase in the 11 adolescents and adults who had worsening in at least one biochemical parameter on favipiravir.

<p>(A) Patients who survived (<i>n</i> = 7). (B) Patients who died (<i>n</i> = 4). Each line represents one patient. All patients are identified with an ID number (from 1 to 7) or letter (from a to d) throughout the figures. For all 11 patients, all available data are shown. Dots represent baseline values, and X’s represent follow-up values. Dark blue lines represent patients with baseline Ct < 20 who survived. Light blue lines represent patients with baseline Ct ≥ 20 who survived. Light red lines represent patients with baseline Ct ≥ 20 who died. The <i>x</i>-axis represents the time since first symptoms (for example, for a patient whose first symptom occurred 5 d before admission to the treatment center, the baseline value dot is positioned at 5 d). Samples obtained more than 25 d after onset of symptoms are not represented. All 11 patients continued favipiravir.</p

JIKI trial progress.

<p>ALIMA, Alliance for International Medical Action; CRF, Croix Rouge Française (French Red Cross); EU, European Union; Inserm, Institut National de la Santé et de la Recherche Médicale; SSA, Service de Santé des Armées (French military health service).</p