High Temporal Resolution XMM Monitoring of PKS 2155-304

The bright, strongly variable BL Lac object PKS 2155-304 was observed by XMM for two essentially uninterrupted periods of ~11 and 16 hr on 30-31 May 2000. The strongest variations occurred in the highest energy bands. After scaling for this effect, the three softest bands (0.1-1.7 keV) showed strong correlation with no measurable lag to reliable limits of \tau \ls 0.3 hr. However, the hardest band (~3 keV) was less well-correlated with the other three, especially on short time scales, showing deviations of ~10-20% in ~1 hr although, again, no significant interband lag was detected. This result and examination of previous ASCA and BeppoSAX cross-correlation functions suggest that previous claims of soft lags on time scales of 0.3-4 hr could well be an artifact of periodic interruptions due to Earth-occultation every 1.6 hr. Previous determinations of the magnetic field/bulk Lorentz factor were therefore premature, as these data provide only a lower limit of B \gamma^{1/3} \gs 2.5 G. The hardest band encompasses the spectral region above the high-energy break; its enhanced variability could be indicating that the break energy of the synchrotron spectrum, and therefore of the underlying electron energy distribution, changes independently of the lower energies.Comment: 13 pages, 3 figures, accepted by Ap

Inflationary Cosmological Perturbations of Quantum-Mechanical Origin

This review article aims at presenting the theory of inflation. We first describe the background spacetime behavior during the slow-roll phase and analyze how inflation ends and the Universe reheats. Then, we present the theory of cosmological perturbations with special emphasis on their behavior during inflation. In particular, we discuss the quantum-mechanical nature of the fluctuations and show how the uncertainty principle fixes the amplitude of the perturbations. In a next step, we calculate the inflationary power spectra in the slow-roll approximation and compare these theoretical predictions to the recent high accuracy measurements of the Cosmic Microwave Background radiation (CMBR) anisotropy. We show how these data already constrain the underlying inflationary high energy physics. Finally, we conclude with some speculations about the trans-Planckian problem, arguing that this issue could allow us to open a window on physical phenomena which have never been probed so far.Comment: Review Article, 47 pages, 3 figures. Lectures given at the 40th Karpacz Winter School on Theoretical Physics (Poland, Feb. 2004), submitted to Lecture Notes in Physic

Dependence of Inflationary Reconstruction upon Cosmological Parameters

The inflationary potential and its derivatives determine the spectrum of scalar and tensor metric perturbations that arise from quantum fluctuations during inflation. The CBR anisotropy offers a promising means of determining the spectra of metric perturbations and thereby a means of constraining the inflationary potential. The relation between the metric perturbations and CBR anisotropy depends upon cosmological parameters -- most notably the possibility of a cosmological constant. Motivated by some observational evidence for a cosmological constant (large-scale structure, cluster-baryon fraction, measurements of the Hubble constant and age of the Universe) we derive the reconstruction equations and consistency relation to second order in the presence of a cosmological constant. We also clarify previous notation and discuss alternative schemes for reconstruction.Comment: 15 pages, LaTeX, 3 postscript figures (included with epsf), submitted to Phys. Rev.

Intense Sweeteners, Taste Receptors and the Gut Microbiome: A Metabolic Health Perspective

Intense sweeteners (IS) are often marketed as a healthier alternative to sugars, with the potential to aid in combating the worldwide rise of diabetes and obesity. However, their use has been counterintuitively associated with impaired glucose homeostasis, weight gain and altered gut microbiota. The nature of these associations, and the mechanisms responsible, are yet to be fully elucidated. Differences in their interaction with taste receptors may be a potential explanatory factor. Like sugars, IS stimulate sweet taste receptors, but due to their diverse structures, some are also able to stimulate bitter taste receptors. These receptors are expressed in the oral cavity and extra-orally, including throughout the gastrointestinal tract. They are involved in the modulation of appetite, glucose homeostasis and gut motility. Therefore, taste genotypes resulting in functional receptor changes and altered receptor expression levels may be associated with metabolic conditions. IS and taste receptors may both interact with the gastrointestinal microbiome, and their interactions may potentially explain the relationship between IS use, obesity and metabolic outcomes. While these elements are often studied in isolation, the potential interactions remain unexplored. Here, the current evidence of the relationship between IS use, obesity and metabolic outcomes is presented, and the potential roles for interactions with taste receptors and the gastrointestinal microbiota in modulating these relationships are explored

An extragalactic supernebula confined by gravity

Little is known about the origins of the giant star clusters known as globular clusters. How can hundreds of thousands of stars form simultaneously in a volume only a few light years across the distance of the sun to its nearest neighbor? Radiation pressure and winds from luminous young stars should disperse the star-forming gas and disrupt the formation of the cluster. Globular clusters in our Galaxy cannot provide answers; they are billions of years old. Here we report the measurement of infrared hydrogen recombination lines from a young, forming super star cluster in the dwarf galaxy, NGC 5253. The lines arise in gas heated by a cluster of an estimated million stars, so young that it is still enshrouded in gas and dust, hidden from optical view. We verify that the cluster contains 4000-6000 massive, hot "O" stars. Our discovery that the gases within the cluster are bound by gravity may explain why these windy and luminous O stars have not yet blown away the gases to allow the cluster to emerge from its birth cocoon. Young clusters in "starbursting" galaxies in the local and distant universe may be similarly gravitationally confined and cloaked from view.Comment: Letter to Natur

Determination of neo- and d-chiro-Inositol Hexakisphosphate in Soils by Solution 31P NMR Spectroscopy

The inositol phosphates are an abundant but poorly understood group of organic phosphorus compounds found widely in the environment. Four stereoisomers of inositol hexakisphosphate (IP6) occur, although for three of these (scyllo, flea, and D-chiro) the origins, dynamics, and biological function remain unknown, due in large part to analytical limitations in their measurement in environmental samples. We synthesized authentic neo- and n-chiro-IP6 and used them to identify signals from these compounds in three soils from the Falkland Islands. Both compounds resisted hypobromite oxidation and gave quantifiable P-31 NMR signals at delta = 6.67 ppm (equatorial phosphate groups of the 4-equatorial/2-axial conformer of neo-IP6) and delta = 6.48 ppm (equatorial phosphate groups of the 2-equatorial/4-axial conformer of D-chiro-IP6) in soil extracts. Inositol hexakisphosphate accounted for 46-54% of the soil organic phosphorus, of which the four stereoisomers constituted, on average, 55.9% (myo), 32.8% (scyllo), 6.1% (neo), and 5.2% (n-chiro). Reappraisal of the literature based on the new signal assignments revealed that neo- and D-chiro-IP6 occur widely in both terrestrial and aquatic ecosystems. These results confirm that the inositol phosphates can constitute a considerable fraction of the organic phosphorus in soils and reveal the prevalence of neo- and D-chiro-IP6 in the environment. The hypobromite oxidation and solution P-31 NMR spectroscopy procedure allows the simultaneous quantification of all four IP6 stereoisomers in environmental samples and provides a platform for research into the origins and ecological significance of these enigmatic compounds

Unlocking biocatalytic acylations by enzyme repurposing and engineering for amide synthesis

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A Critical Role for Syk in Signal Transduction and Phagocytosis Mediated by Fcγ Receptors on Macrophages

Receptors on macrophages for the Fc region of IgG (FcγR) mediate a number of responses important for host immunity. Signaling events necessary for these responses are likely initiated by the activation of Src-family and Syk-family tyrosine kinases after FcγR cross-linking. Macrophages derived from Syk-deficient (Syk−) mice were defective in phagocytosis of particles bound by FcγRs, as well as in many FcγR-induced signaling events, including tyrosine phosphorylation of a number of cellular substrates and activation of MAP kinases. In contrast, Syk− macrophages exhibited normal responses to another potent macrophage stimulus, lipopolysaccharide. Phagocytosis of latex beads and Escherichia coli bacteria was also not affected. Syk− macrophages exhibited formation of polymerized actin structures opposing particles bound to the cells by FcγRs (actin cups), but failed to proceed to internalization. Interestingly, inhibitors of phosphatidylinositol 3-kinase also blocked FcγR-mediated phagocytosis at this stage. Thus, PI 3-kinase may participate in a Syk-dependent signaling pathway critical for FcγR-mediated phagocytosis. Macrophages derived from mice deficient for the three members of the Src-family of kinases expressed in these cells, Hck, Fgr, and Lyn, exhibited poor Syk activation upon FcγR engagement, accompanied by a delay in FcγR-mediated phagocytosis. These observations demonstrate that Syk is critical for FcγR-mediated phagocytosis, as well as for signal transduction in macrophages. Additionally, our findings provide evidence to support a model of sequential tyrosine kinase activation by FcγR's analogous to models of signaling by the B and T cell antigen receptors