HPLC elution profiles of four fecal extracts from a Bengal tiger collected on Days 89, 97, 98 and 100 (a) and from a jaguar collected on Days 70, 73, 76 and 77 (b).

<p>Immunoreactivitiy of each fraction was determined in the PGFM EIA, arrows represent the elution positions of reference standards.</p

HPLC elution profile of immunoreactive PGFM in fecal extracts of a pregnant tiger (days 97, a) and a pregnant jaguar (days 76, b).

<p>Fecal extracts of each animal were separated by RP-HPLC and then immunoreactivitiy of each fraction was determined by the PGFM EIA. Arrows indicate the elution positions of prostaglandin standards.</p

Term-wise PGFM concentration measured by EIA in Asiatic lion, Bengal tiger and jaguar.

<p>To examine differences in PGFM concentrations between different gestation periods, we divided pregnancy into three equal periods as early (1–5 weeks), mid (6–10 weeks) and late term (11–15 weeks) for Asiatic lion, Bengal tiger and jaguar.</p

FGM and environmental data, roe deer

Full database of fecal glucucocorticoid metabolites (FGM) concentration in pellets of roe deer (Capreolus capreolus) collected in 6 areas in Poland along with environmental data (see text for details on sources of data)

Determining hormone metabolite concentrations when enzyme immunoassay accuracy varies over time

<p>Data for identifying clusters of samples assayed with similar enzyme immunoassay accuracy (data_for_cluster.txt), modelling the relationship between initial and remeasured metabolite concentrations in a subset of 27 samples (data_for_fit.txt), and applying the standardisation procedure using the complete data set of 138 samples (data_for_standardisation.txt).</p

HPLC profiles of immunoreactive testosterone metabolites in captive hyenas.

<p>Testosterone immunoreactivity of faecal extracts were analysed in faecal extracts of one captive adult male (A) and one captive adult female (B) spotted hyena. Immunoreactivity was determined in the epiandrosterone EIA and is presented as a percentage of overall eluted activity. Lines with black circles represent immunoreactivity in each fraction. Lines with white circles show immunoreactivity in the fractions of the same extract after hydrolysis. The arrows represent the elution positions of reference standards cortisol (1), corticosterone (2), testosterone (3), epiandrosterone (4) and dihydrotestosterone (5).</p

Comparison of faecal cortisol and testosterone immunoreactivity.

<p>Changes in fGM and fTM concentrations were determined in faecal samples from the ACTH challenge following hydrolysis with β-glucuronidase from <i>Helix pomatia</i> in the cortisol-3CMO, epiandrosterone and testosterone-11-HS EIAs, respectively. Levels of fGM and fTM are shown as percentage increase over pre-injection levels.</p