Genetic engineering of baker’s and wine yeasts using formaldehyde hyperresistance-mediating plasmids

Yeast multi-copy vectors carrying the for maldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker’s and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting agent will be consumed and therefore its removal during down-stream processing is not necessary. Thus, this vector provides an expression system which is simple to apply and inexpensive to use. Key words: · Yeast · Transformation · Hyperresistance to formaldehyd

Spin waves cause non-linear friction

Energy dissipation is studied for a hard magnetic tip that scans a soft magnetic substrate. The dynamics of the atomic moments are simulated by solving the Landau-Lifshitz-Gilbert (LLG) equation numerically. The local energy currents are analysed for the case of a Heisenberg spin chain taken as substrate. This leads to an explanation for the velocity dependence of the friction force: The non-linear contribution for high velocities can be attributed to a spin wave front pushed by the tip along the substrate.Comment: 5 pages, 9 figure

Spin waves cause non-linear friction

Energy dissipation is studied for a hard magnetic tip that scans a soft magnetic substrate. The dynamics of the atomic moments are simulated by solving the Landau-Lifshitz-Gilbert (LLG) equation numerically. The local energy currents are analysed for the case of a Heisenberg spin chain taken as substrate. This leads to an explanation for the velocity dependence of the friction force: The non-linear contribution for high velocities can be attributed to a spin wave front pushed by the tip along the substrate.Comment: 5 pages, 9 figure

Topological phase transitions and chiral inelastic transport induced by the squeezing of light

We show how the squeezing of light can lead to the formation of topological states. Such states are characterized by non-trivial Chern numbers, and exhibit protected edge modes which give rise to chiral elastic and inelastic photon transport. These topological bosonic states are not equivalent to their fermionic (topological superconductor) counterparts and cannot be mapped by a local transformation onto topological states found in particle-conserving models. They thus represent a new type of topological system. We study this physics in detail in the case of a Kagome lattice model, and discuss possible realizations using nonlinear photonic crystals or superconducting circuits.Comment: 11 pages, 4 figure

Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1

The conditionally-lethal pso4-1 mutant allele of the spliceosomal-associated PRP19 gene allowed us to study this gene’s influence on pre-mRNA processing, DNA repair and sporulation. Phenotypes related to intron-containing genes were correlated to temperature. Splicing reporter systems and RT–PCR showed splicing efficiency in pso4-1 to be inversely correlated to growth temperature. A single amino acid substitution, replacing leucine with serine, was identified within the N-terminal region of the pso4-1 allele and was shown to affect the interacting properties of Pso4-1p. Amongst 24 interacting clones isolated in a two-hybrid screening, seven could be identified as parts of the RAD2, RLF2 and DBR1 genes. RAD2 encodes an endonuclease indispensable for nucleotide excision repair (NER), RLF2 encodes the major subunit of the chromatin assembly factor I, whose deletion results in sensitivity to UVC radiation, while DBR1 encodes the lariat RNA splicing debranching enzyme, which degrades intron lariat structures during splicing. Characterization of mutagen-sensitive phenotypes of rad2{Delta}, rlf2{Delta} and pso4-1 single and double mutant strains showed enhanced sensitivity for the rad2{Delta} pso4-1 and rlf2{Delta} pso4-1 double mutants, suggesting a functional interference of these proteins in DNA repair processes in Saccharomyces cerevisiae

Spin excitations in a monolayer scanned by a magnetic tip

Energy dissipation via spin excitations is investigated for a hard ferromagnetic tip scanning a soft magnetic monolayer. We use the classical Heisenberg model with Landau-Lifshitz-Gilbert (LLG)-dynamics including a stochastic field representing finite temperatures. The friction force depends linearly on the velocity (provided it is small enough) for all temperatures. For low temperatures, the corresponding friction coefficient is proportional to the phenomenological damping constant of the LLG equation. This dependence is lost at high temperatures, where the friction coefficient decreases exponentially. These findings can be explained by properties of the spin polarization cloud dragged along with the tip.Comment: 6 pages, 5 figure