The Role of the County Professional Council in Advanced Training and Professional Development of Class Teachers

Stručno usvršavanje je obvezan dio učiteljskog posla koji se provodi na četiri osnovne razine: individualnoj, školskoj, županijskoj i državnoj. Brojne su prednosti i nedostaci svakog oblika usavršavanja, a svi su oni na putu profesionalnog razvoja učitelja jednako važni i korisni. U radu je prikazan model stručnog usvršavanja učitelja na županijskoj razini (Županijsko stručno vijeće učitelja razredne nastave – Grad Sisak) pri čemu veliku ulogu imaju upravo županijski voditelji koji su poveznica između školske i državne razine stručnog usavršavanja učitelja.Advanced training is a mandatory part of teacher’s job and it is being carried out at four basic levels: individual, school, county and state level. There are numerous advantages and disadvantages of any form of training, and they are all equally important and useful in the course of professional development of teachers. This study presents the model of advanced training of class teachers at county level (County professional council of class teachers – the Town of Sisak), where the very county leaders, who are the link between the school and state level of advanced training of teachers, have a great role

Three types of <i>agr</i> loci are present in different strains of <i>C. difficile</i> and phiCDHM1.

<p>Three types of <i>agr</i> loci have been identified. The <i>agr1</i> locus encodes AgrB and AgrD: the a<i>gr2</i> locus encodes AgrA (containing a LytTR protein domain), AgrC (with homology to VirS), AgrD and AgrB: the <i>agr3</i> locus encodes the AgrC, AgrB and AgrD. Symbols; empty circle, full circle and diamond are in reference to the taxa clusters in the ML phylogenetic analysis in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085131#pone-0085131-g005" target="_blank">Figures 5</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085131#pone-0085131-g007" target="_blank">7</a>.</p

ML phylogenetic analysis of related <i>agrC</i> genes from phiCDHM1 and <i>C. difficile</i> strains.

<p>Phylogenetic analysis was performed on homologs of <i>agrC</i> in the <i>agr1</i> and <i>agr3</i> loci of <i>C. difficile</i> strains in the NCBI genome db (Oct 2011) and the <i>agrC</i> gene from <i>S. aureus subsp. aureus MRSA252</i>. The translated sequences were aligned with MUSCLE and ML analysis performed using parameters set for the JTT nucleotide substitution model, with invariant rates, using all sites and CNI for Tree Inference and bootstrapped with 500 replicates in MEGAv5.01. Symbols correspond to those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085131#pone-0085131-g004" target="_blank">Figure 4</a> and indicate the type of <i>agr</i> locus in which the gene is present (either <i>agr1</i>, <i>agr2</i> or <i>agr3</i>). Taxa are abbreviated to strain names and number indicates locus type.</p

ML phylogenetic analysis of the <i>agrD</i> gene from phiCDHM1 and <i>C. difficile</i> strains.

<p>Phylogenetic analysis was performed on the <i>agrD</i> genes of phiCDHM1 and sequenced <i>C. difficile</i> strains in the NCBI genome db (Oct 2011) and <i>agrD</i> of <i>S. aureus subsp. aureus MRSA252</i>. The translated sequences were aligned with MUSCLE and ML analysis performed using parameters set for the JTT nucleotide substitution model, with invariant rates, using all sites and CNI for Tree Inference and bootstrapped with 500 replicates in MEGAv5.01. Symbols correspond to those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085131#pone-0085131-g004" target="_blank">Figure 4</a> and indicate the type of <i>agr</i> locus in which the gene is present (either <i>agr1</i>, <i>agr2</i> or <i>agr3</i>). Taxa are abbreviated to strain names and number indicates locus type.</p

Particle morphology of phiCDHM1.

<p>TEM analysis of phiCDHM1 shows it to belong to the <i>Myoviridae</i> with an icosahedral capsid ∼60 nm, contractile tail sheath ∼110 nm length and ∼20 nm diameter and visible tail fibers. Scale bar is 100 nm.</p

Genome map of phiCDHM1.

<p>Linear map showing position of CDSs to scale and annotated with predicted function. CDSs are coloured according to their GC content as shown in key.</p

Mechanism of phage release from microparticles.

<p>Schematic of ai) the chemical structure of Eudragit® S100, y:x = 2:1 aii) Eudragit® S100 polymer chain separation due to dissociation of weakly acidic functional groups, electrostatic repulsion and polymer swelling; (b) Phage loading/release mechanism from pH responsive Eudragit® S100 microparticles.</p

Influence of formulation parameters on free phage viability at various exposure times.

<p>a) effect of pH on free phage viability in 0.2 M NaCl solution. * indicates significantly different phage titres using a 2 sample t-test at each condition compared with phage in BHI (controls) at T0 (p < 0.05). Error bars indicate 95% confidence intervals for means.; b) effect of polymer solvent on free phage viability. T0 point denotes time between 0–10 min, T6 exposure to solution for 6h and T24 exposure for 24h. * indicates significantly different phage titres using a 2 sample t-test at each condition compared with phage at T0 for each composition (p < 0.05). Error bars indicate 95% confidence intervals for means.</p

Long term stability study of a glass capillary microfluidic device in generating water-in-oil droplets.

<p><b>Aqueous phase: 1% (w/v) Eudragit® S100 and oil phase: 2% (w/v) PGPR in Miglyol 840.</b> (a) Snapshots of droplet generation over six hours (b) Device function over six hours with corresponding droplet size and coefficient of variation (CV) (D_d = ◆; CV = ☐). Q_d = 1.2 ml/hr, Q_c = 7 ml/hr, orifice size = 200 μm). Error bars represent one standard deviation (20 droplets counted per time point).</p

Microfluidic production of microparticles.

<p>a) Schematic of laboratory experimental set-up; b) Schematic of droplet production using a glass capillary microfluidic device followed by in situ gelation with 4-aminobenzoic acid dissolved in the oil phase; c) Schematic of the plug flow regime due to premature gelation of Eudragit® S100 with p-toluenesulfonic acid resulting in a high viscosity of the aqueous phase during jet pinch-off. <i>p</i>-<i>Toluenesulfonic acid</i>, with a pKa of −2.8, is more than 10,000 <i>times stronger</i> acid than 4-aminobenzoic acid, with a pKa of 2.38.</p