ROC of putative prostate cancer biomarkers.

<p>ROC curve demonstrates the discriminating performance of the protein expression in the differentiation between malignant and non-malignant tissue cores using the area fraction (probit) data for BTF3, HINT1, NDRG1 and ODC1. The operating characteristic values are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084295#pone.0084295.s005" target="_blank">Table S1</a>. The solid line represents the ROC area of 0.5. </p

Multi-labeled immunofluorescence for BTF3, HINT1 and NDRG1.

<p>Co-expression of three proteins BTF3 (FITC-green), HINT1 (Cy3- blue) and NDRG1 (Cy5-red) in non-malignant (A) and malignant prostate tissue cores (B); images are representative tissue cores from the tissue array with over 200 samples. The three antibodies were incubated simultaneously and labeled with three Cy5 –red different fluorophores using Bond automated staining system. Whole tissue cores were imaged using a Leica confocal system and 4x4 tiling at exactly the same settings for comparison. Images were zoomed 3x using digital zoom in ImageJ. Scale bar =100µm.</p

Prognostic clinicopathologic variables as predictors of disease-specific survival in 335 NSCLC patients (univariate analyses; log-rank test).

<p>NR, not reached.</p><p>Prognostic clinicopathologic variables as predictors of disease-specific survival in 335 NSCLC patients (univariate analyses; log-rank test).</p

Results of Cox regression analyses (backward stepwise model) for clinicopathological factors and monocarboxylate transporters (MCTs) (model 1) and metabolic co-expression variables (*model 2).

<p>Results of Cox regression analyses (backward stepwise model) for clinicopathological factors and monocarboxylate transporters (MCTs) (model 1) and metabolic co-expression variables (*model 2).</p

Quantitation of DAB signal in prostate tissue array.

<p>An unbiased, automated protocol was used to calculate Total Area stained (in pixel) standardized to amount of tissue in each core (see Methods). TA is converted to Area Fraction (total area divided by the total pixels in the image). Each bin is data for an individual, malignant (red) or non-malignant tissue (green) core. There is a significant increase in the AUC of protein expression in malignant v non-malignant prostate tissue (p<0.0001). </p

Immunhistochemical staining of MCT1–4 in NSCLC.

<p>Low expression: A) MCT1, B) MCT2, C) MCT3, D) MCT4. High expression: E) MCT1, F) MCT2, G) MCT3, H) MCT4. 100× and 400× magnification.</p

Kaplan Meier curves with DSS for expression of MCT1 and co-expression variables.

<p>Kaplan Meier curves with disease-specific survival for expression of monocarboxylate transporter (MCT)1 in cancer cells and stromal cells and the co-expression variable GLUT1c+MCT4c and GLUT1c+MCT1s in NSCLC. A) MCT1 in cancer cells, B) MCT1 in stromal cells, C) GLUT1c+MCT4c, D) GLUT1c+MCT1s.</p

Metabolic co-expression variables in cancer and stromal cells as predictors of disease-specific survival in 335 NSCLC patients (univariate analyses; log-rank test).

<p>Metabolic co-expression variables in cancer and stromal cells as predictors of disease-specific survival in 335 NSCLC patients (univariate analyses; log-rank test).</p