<p>Co-expression of three proteins BTF3 (FITC-green), HINT1 (Cy3- blue) and NDRG1 (Cy5-red) in non-malignant (A) and malignant prostate tissue cores (B); images are representative tissue cores from the tissue array with over 200 samples. The three antibodies were incubated simultaneously and labeled with three Cy5 –red different fluorophores using Bond automated staining system. Whole tissue cores were imaged using a Leica confocal system and 4x4 tiling at exactly the same settings for comparison. Images were zoomed 3x using digital zoom in ImageJ. Scale bar =100µm.</p
