Plant extracts of Cleome viscosa L. as biostimulants of the in vitro germination of two varieties of pepper (Capsicum annuum L.)

The aim of the work was to evaluate the biostimulant effect of plant extracts of Cleome viscosa L. on the in vitro germination of two varieties of pepper (Capsicum annuum L.). A bioassay was set up on a completely randomized design with a control and 18 treatments with three samples each, evaluating the effect on the dynamics and percentage of germination, radicle length and hypocotyl length. The chemical families present in the extracts were determined by phytochemical characterization. For the length of the radicle and the hypocotyl, the extracts with the best potentialities in the Verano 1 variety are those obtained from the root and stem, although the concentrations with the best influence on both indicators are 1 and 3 of the root extracts. Differences in the richness of chemical families were verified in the extracts with the best results, although the best composition was obtained in those of the root and stem, showing the presence of free amino acids in all cases. Cleome viscosa is promising for obtaining products with a biostimulant effect on the varieties of pepper studied, obtaining a better effect when root, stem and whole plant extracts are applied

Intraoperative and postoperative anesthesia-related complications of percutaneous nephrolithotomy in patients with coraliform lithiasis

Introduction: Renal lithiasis currently accounts for 20-30% of urology consultations worldwide. Objectives: To identify the intra- and postoperative complications of percutaneous nephrolithotomy in patients with staghorn lithiasis. Methods: A descriptive study was conducted in patients with a diagnosis of staghorn lithiasis and admitted for elective percutaneous nephrolithotomy Hermanos Ameijeiras Clinical-Surgical Hospital between October 2010 and October 2015. Results: We identified 32 patients with intra- and postoperative complications. The male sex was represented by the 68.7%. The mean age was 47.59±12.2 years. The most frequent ASA classification was ASA II, accounting for 56.2%. All patients received general anesthesia. The mean value of intraoperative hematic losses was 650.00±413.09 mL. Total volume replacement was 10, 107.80±2, 659.25 mL. Chloride sodium 0.9% was administered at doses 7743.75±2007.39 mL and packed red blood cells to 18.8% of the total. Intraoperative complications occurred in 24 patients, accounting for 75.0%. Of these, the most frequent were metabolic, cardiovascular, respiratory and renal. Hypothermia was present in one third of the patients. Postoperative complications occurred in 23 patients, accounting for 71.9%. Of these, cardiovascular, respiratory, renal and septic were the most frequent. Conclusions: The complications were frequent and serious. However, an important percentage number was achieved for cured discharge patients. </span

Strategy for the purification of soluble fibronectin from human plasma, as ligand for affinity chromatography

El presente trabajo se desarrolló con el objetivo de purificar y caracterizar fibronectina soluble procedente de plasma para su posterior aplicación en la purificación de proteínas de adhesión. Se inmovilizó gelatina en Sefarosa 2BCL como soporte cromatográfico para la preparación de una columna. Se estudiaron diferentes alternativas de elución con arginina 0.5 M y 1M, con glicina -NaCl, acetato de sodio - NaCl y urea - NaCl, lo cual confirmó las ventajas en el uso de la arginina como agente eluyente. Se incorporó una segunda etapa de purificación en una columna de Heparina - Sefarosa 4B CNBr a partir de la que se obtuvo fibronectina con alto grado de pureza en estado nativo, comprobado por SDS-PAGE. La proteína purificada se inmovilizó en una matriz de Sefarosa con un grado de sustitución de 0,98 mg fibronectina/mL de gel lo cual permitió una capacidad de adsorción de gelatina libre con una recuperación de 8 mg (0,64 mg gelatina/mL de gel). La utilización de esta matriz permitirá su aplicación preliminar en la purificación de proteínas de adhesión que podrían ser usadas como antígenos en vacunas contra leptospirosis.Soluble fibronectin from human plasma was purified and characterized in order to be applied in the purification of adhesion protein. Gelatin was immobilized in a chromatographic bed as Sepharose 2B CL to prepare a column. Different alternatives of elution with 0.5 M and 1 M arginin, glicin- NaCl, sodium acetate - NaCl and urea - NaCl buffers, confirmed the advantages of the arginin as elution solution in this column. Second step of purification in a column of Heparin - Sepharose 4B CNBr was included to obtain the native fibronectin with high purity degree, verified by SDS-PAGE. Purified protein was immobilized in a matrix of Sepharose with a substitution grade of 0.98 mg fibronectin/mL of gel that permited an adsorption capacity of free gelatin to the column of 8 mg (0.64 mg gelatin/mL of gel). This matrix will allow its preliminary application in the purification of adhesion proteins which could be used as antigens in vaccines againt leptospirosis.Colegio de Farmacéuticos de la Provincia de Buenos Aire