Pathological changes and viral antigen distribution in tissues of Iberian hare (Lepus granatensis) infected with myxoma virus

Resumen del trabajo presentado a la Joint Virtual Conference of the WDA and EWDA, celebrada del 31 de agosto al 2 de septiembre de 2021.Peer reviewe

Seroepidemiological study of Toxoplasma gondii in wild and domestic lagomorphs in Spain

Resumen del trabajo presentado a la Joint Virtual Conference of the WDA and EWDA, celebrada del 31 de agosto al 2 de septiembre de 2021.Peer reviewe

Fungal Planet description sheets: 868-950

Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernambucoensis from soil, Calonectria matogrossensis on Eucalyptus urophylla leaves, Calvatia brasiliensis on soil, Carcinomyces nordestinensis on Bromelia antiacantha leaves, Dendryphiella stromaticola on small branches of an unidentified plant, Nigrospora brasiliensis on Nopalea cochenillifera leaves, Penicillium alagoense as a leaf endophyte on a Miconia sp., Podosordaria nigrobrunnea on dung, Spegazzinia bromeliacearum as a leaf endophyte on Tilandsia catimbauensis, Xylobolus brasiliensis on decaying wood. Bulgaria, Kazachstania molopis from the gut of the beetle Molops piceus. Croatia, Mollisia endocrystallina from a fallen decorticated Picea abies tree trunk. Ecuador, Hygrocybe rodomaculata on soil. Hungary, Alfoldia vorosii (incl.Alfoldia gen. nov.) from Juniperus communis roots, Kiskunsagia ubrizsyi (incl. Kiskunsagia gen. nov.) from Fumana procumbens roots. India, Aureobasidium tremulum as laboratory contaminant, Leucosporidium himalayensis and Naganishia indica from windblown dust on glaciers. Italy, Neodevriesia cycadicola on Cycas sp. leaves, Pseudocercospora pseudomyrticola on Myrtus communis leaves, Ramularia pistaciae on Pistacia lentiscus leaves, Neognomoniopsis quercina (incl. Neognomoniopsis gen. nov.) on Quercus ilex leaves. Japan, Diaporthe fructicola on Passiflora edulis × P. edulis f. flavicarpa fruit, Entoloma nipponicum on leaf litter in a mixed Cryptomeria japonica and Acer spp. forest. Macedonia, Astraeus macedonicus on soil. Malaysia, Fusicladium eucalyptigenum on Eucalyptus sp. twigs, Neoacrodontiella eucalypti (incl. Neoacrodontiella gen. nov.) on Eucalyptus urophylla leaves. Mozambique, Meliola gorongosensis on dead Philenoptera violacea leaflets. Nepal, Coniochaeta dendrobiicola from Dendriobium lognicornu roots. New Zealand, Neodevriesia sexualis and Thozetella neonivea on Archontophoenix cunninghamiana leaves. Norway, Calophoma sandfjordenica from a piece of board on a rocky shoreline, Clavaria parvispora on soil, Didymella finnmarkica from a piece of Pinus sylvestris driftwood. Poland, Sugiyamaella trypani from soil. Portugal, Colletotrichum feijoicola from Acca sellowiana. Russia, Crepidotus tobolensis on Populus tremula debris, Entoloma ekaterinae, Entoloma erhardii and Suillus gastroflavus on soil, Nakazawaea ambrosiae from the galleries of Ips typographus under the bark of Picea abies. Slovenia, Pluteus ludwigii on twigs of broadleaved trees. South Africa, Anungitiomyces stellenboschiensis (incl. Anungitiomyces gen. nov.) and Niesslia stellenboschiana on Eucalyptus sp. leaves, Beltraniella pseudoportoricensis on Podocarpus falcatus leaf litter, Corynespora encephalarti on Encephalartos sp. leaves, Cytospora pavettae on Pavetta revoluta leaves, Helminthosporium erythrinicola on Erythrina humeana leaves, Helminthosporium syzygii on a Syzygium sp. barkcanker, Libertasomyces aloeticus on Aloe sp. leaves, Penicillium lunae from Musa sp. fruit, Phyllosticta lauridiae on Lauridia tetragona leaves, Pseudotruncatella bolusanthi (incl. Pseudotruncatellaceae fam. nov.) and Dactylella bolusanthi on Bolusanthus speciosus leaves. Spain, Apenidiella foetida on submerged plant debris, Inocybe grammatoides on Quercus ilex subsp. ilex forest humus, Ossicaulis salomii on soil, Phialemonium guarroi from soil. Thailand, Pantospora chromolaenae on Chromolaena odorata leaves. Ukraine, Cadophora helianthi from Helianthus annuus stems. USA, Boletus pseudopinophilus on soil under slash pine, Botryotrichum foricae, Penicillium americanum and Penicillium minnesotense from air. Vietnam, Lycoperdon vietnamense on soil. Morphological and culture characteristics are supported by DNA barcodes

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p < 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

A novel bridgehead azocine. The end of a controversy

A novel bridgehead azocine has been obtained from aminopyran 1 and benzylidinemalononitrile . The structure of was confirmed by X-ray diffraction. A novel bridgehead azocine has been obtained and the structure confirmed by X-ray diffraction.The authors wish to thank the Commision Interministerial de Ciencia y Tecnologia of Spain for financial support (Grants PB89-0495 and PB87-0291)

The Crystal and Molecular Structure of BNA, a Cyclotetracondensate from Quaternary Salts of Nicotinamide

Four molecules of quaternary salts of nicotinamide cyclocondense to form a new product (BNA). The crystals of the title compound (C52H48NsO4) are monoclinic, space group P21/c, with Z = 4, a = 14.357 (3), b = 10.736 (1), c = 34.017 (6) A and/~ = 123.38 (3) °. The structure has been determined by direct and Fourier methods and refined by least-squares analysis to an R value of 0.067 for 2472 observed reflexions. Four intramolecular hydrogen bonds involving the O atoms may explain the lipophilic properties of the molecule

Mesa redonda sobre investigadores y Ciencia Abierta

Mesa redonda de las 5ª Jornadas de Análisis de la Red de Bibliotecas y Archivos del CSIC (Bibliotecas y Archivos del CSIC por la Ciencia Abierta: presente y futuro), celebradas en Madrid los días 28 y 29 de noviembre de 2019.-- Eje 1: Ciencia Abierta: concepto, elementos y estrategias.Modera. Félix Alonso (Real Jardín Botánico. RJB-CSIC). Participantes: Emilio Cano (Centro Nacional de Investigaciones Metalúrgicas. CENIM-CSIC), María Paz Martín (Real Jardín Botánico. RJB-CSIC), José María Carazo (Centro Nacional de Biotecnología. CNB-CSIC) y Jan Thiele (Centro de Ciencias Humanas y Sociales-Instituto de Lenguas y Culturas del Mediterráneo y Oriente Próximo. CCHS-ILC-CSIC).N

Experimental evidence of deviations from a karplus-like relationship of vicinal carbon-proton coupling constants in some conformationally rigid carbohydrate derivatives

The values of vicinal carbon-proton coupling constants for the conformationally rigid carbohydrate derivatives N-acetyl-3,4-di-O-acetyl-1,2-dideoxy-β-D-hexo(pento)pyranosyl[1,2-d] oxazolidin-2′-ones (1-4), 2,4-di-O-acetyl-1-acetamido-1-deoxy-1-N,3-O-carbonyl-α-L-arabinopyranose (5), and 2,3,4-tri-O-acetyl-1,6-anhydro-β-D-hexopyranoses (6-12) are reported. 3JC,H values were determined by the selective 2D heteronuclear J-resolved method proposed by Bax and Freeman. The values found were used as input parameters for simulating the coupled carbon spectra. From the final best values it may be concluded that both the number and relative orientation of substituents in a complex organic molecule may substantially modify the general shape of the existing Karplus-like relationship between 3JC,H and the corresponding dihedral angle. The crystal structures of N-acetyl-3,4-di-O-acetyl-1,2-dideoxy-β-D-gluco- and -xylopyranosyl[1,2-d]oxazolidin-2′-ones (1 and 3) and compound 5 have also been studied in order to determine the geometry of these molecules in the solid state.We thank the CAICYT and CSIC for financial support, the Ministerio de Educación for a fellowship (J.J.-B.), M. C.

La Ciencia en el Desarrollo Tecnológico de Envases

Se presenta la situación de los diferentes sectores involucrados en la fabricación de envases de madera, metal, vidrio y PET así como los avances tecnológicos llevados a cabo en los últimos años en relación con los materiales y su fabricación.Plataforma para la Sostenibilidad de los Envases.N

Glycosyl inositol derivatives related to inositolphosphoglycan mediators: Synthesis, structure, and biological activity

17 páginas, 12 figuras, 4 esquemas, 7 tablas.-- Supporting information for this article is available on the WWW under http://www.wiley-vch.de/home/chemistry/ or from the author.The development of effective strategies for the synthesis of phosphatidylinositol precursors such as that which contains the trisaccharide 1, the study of their conformational features and dynamics, and the investigation of their biological properties may result in a better understanding of the mechanism of signal transduction of insulin and a number of growth factors. This new pathway of intracellular signalling has been postulated to proceed through uncharacterized inositolphosphoglycan mediators containing substructures such as 1.Thanks are given to DGICYT (Spain) for financial support (grants no. PB93-0127-C02-01, PB93-0125H and PB93-0098). H. D. is grateful to the Deutsche Forschungsgemeinschaft (DFG) and the European Union (HCM Programme; CARENET-1 Network) for a postdoctoral fellowships. J.-F. E. thanks M.E.C. for a FPU Fellowship. Y. L. is supported by a MEC-CSIC contract.Peer reviewe