GEOCONTEXT AND CHCONTEXT GEOGRAPHIC INFORMATION IN CULTURAL HERITAGE

Internet presence and applications are central for modern museums and cultural institutions. Even more it is important to facilitate and standardize the user experience in order to create a standard quality level and, for the institutions, it is important to simplify configuration operations bound to the creation of the visualized elements and the explorations of the spaces. This demo covers both the technologies underneath the GeoContext tool and the CHContext visualization generator as well as the possible targets and applications. The final elements will cover possible enhancements already in the works

Avaliação do projeto minibibliotecas no Semiárido do Nordeste e no Vale do Jequitinhonha, MG: uma pesquisa-ação.

Introdução; As minibibliotecas no contexto da leitura e do aprendizado; Metodologia; Análise e discussão dos dados.bitstream/item/83404/1/Avaliacao-do-projeto-minibibliotecas.pd

AN IN VIVO MODEL OF HYPERACUTE REJECTION: CHARACTERIZATION AND EVALUATION OF THE EFFECT OF TRANSGENIC HUMAN COMPLEMENT INHIBITORS

Hyperacute rejection (HAR) occurring after transplantation within phylogenetically distant species is a severe reaction triggered by preexisting xenoreactive antibodies and complement activation, leading to the destruction of the donor organ. Expression of human complement inhibitors in transgenic pig organs prolongs the survival of xenograft in experimental models. Moreover, the extent of protection from hyperacute rejection is dependent on the level and site of expression of the transgenic molecules and, probably, on the combination of different molecules. In this regard a small animal model to test the efficacy of expression vectors and different human molecules could be very advantageous. A murine model developed in our laboratory was characterized by measurement of several parameters characteristic of HAR in the livers of control and transgenic mice expressing transgenic human DAF (CD55) or MCP (CD46) at the end of 2 h of perfusion with human plasma and after 1 day. The parameters studied were heamatological values of hepatic functions (GOT and GPT), induction of pro-inflammatory molecules and histopathological evaluation. Cytokines (IL-1 alpha, IL-1 beta, IL-6) induction and exposure of P-selectin on the endothelial cell surface, was only observed in control animals after 2 h of perfusion, as an early event. GOT and GPT values increase drammatically after 2 h perfusion and 1 day after the treatment according to the histopathological observation of liver damage. On the contrary, the livers of hDAF or hMCP transgenic mice, under the same treatment were significantly protected although the extent of this protection is dependent on the level of expression of transgenic human molecules

Dark photon production through positron annihilation in beam-dump experiments

High energy positron annihilation is a viable mechanism to produce dark photons ($A^\prime$). This reaction plays a significant role in beam-dump experiments using experiments using multi-GeV electron-beams on thick targets by enhancing the sensitivity to $A^\prime$ production. The positrons produced by the electromagnetic shower can produce an $A^\prime$ via non-resonant ($e^+ + e^- \to \gamma + A^\prime$) and resonant ($e^+ + e^- \to A^\prime$) annihilation on atomic electrons. For visible decays, the contribution of resonant annihilation results in a larger sensitivity with respect to limits derived by the commonly used $A^\prime$-strahlung in certain kinematic regions. When included in the evaluation of the E137 beam-dump experiment reach, positron annihilation pushes the current limit on $\varepsilon$ downwards by a factor of two in the range 33 MeV/c$^2<m_{A^\prime}<120$ MeV/c$^2$.Comment: 9 pages, 7 figure

Dark matter search with the BDX-MINI experiment

BDX-MINI is a beam dump experiment optimized to search for Light Dark Matter produced in the interaction of the intense CEBAF 2.176 GeV electron beam with the Hall A beam dump at Jefferson Lab. The BDX-MINI detector consists of a PbWO$_4$ electromagnetic calorimeter surrounded by a hermetic veto system for background rejection. The experiment accumulated $2.56 \times 10^{21}$ EOT in six months of running. Simulations of fermionic and scalar Dark Matter interactions with electrons of the active volume of the BDX-MINI detector were used to estimate the expected signal. Data collected during the beam-off time allowed us to characterize the background dominated by cosmic rays. A blind data analysis based on a maximum-likelihood approach was used to optimize the experiment sensitivity. An upper limit on the production of light dark matter was set using the combined event samples collected during beam-on and beam-off configurations. In some kinematics, this pilot experiment is sensitive to the parameter space covered by some of the most sensitive experiments to date, which demonstrates the discovery potential of the next generation beam dump experiment planned at intense electron beam facilities.Comment: (13 pages, 11 figures

GABAergic and Cortical and Subcortical Glutamatergic Axon Terminals Contain CB1 Cannabinoid Receptors in the Ventromedial Nucleus of the Hypothalamus

Background: Type-1 cannabinoid receptors (CB1R) are enriched in the hypothalamus, particularly in the ventromedial hypothalamic nucleus (VMH) that participates in homeostatic and behavioral functions including food intake. Although CB1R activation modulates excitatory and inhibitory synaptic transmission in the brain, CB1R contribution to the molecular architecture of the excitatory and inhibitory synaptic terminals in the VMH is not known. Therefore, the aim of this study was to investigate the precise subcellular distribution of CB1R in the VMH to better understand the modulation exerted by the endocannabinoid system on the complex brain circuitries converging into this nucleus. Methodology/Principal Findings: Light and electron microscopy techniques were used to analyze CB1R distribution in the VMH of CB1R-WT, CB1R-KO and conditional mutant mice bearing a selective deletion of CB1R in cortical glutamatergic (Glu-CB1R-KO) or GABAergic neurons (GABA-CB1R-KO). At light microscopy, CB1R immunolabeling was observed in the VMH of CB1R-WT and Glu-CB1R-KO animals, being remarkably reduced in GABA-CB1R-KO mice. In the electron microscope, CB1R appeared in membranes of both glutamatergic and GABAergic terminals/preterminals. There was no significant difference in the percentage of CB1R immunopositive profiles and CB1R density in terminals making asymmetric or symmetric synapses in CB1R-WT mice. Furthermore, the proportion of CB1R immunopositive terminals/preterminals in CB1R-WT and Glu-CB1R-KO mice was reduced in GABA-CB1R-KO mutants. CB1R density was similar in all animal conditions. Finally, the percentage of CB1R labeled boutons making asymmetric synapses slightly decreased in Glu-CB1R-KO mutants relative to CB1R-WT mice, indicating that CB1R was distributed in cortical and subcortical excitatory synaptic terminals. Conclusions/Significance: Our anatomical results support the idea that the VMH is a relevant hub candidate in the endocannabinoid-mediated modulation of the excitatory and inhibitory neurotransmission of cortical and subcortical pathways regulating essential hypothalamic functions for the individual's survival such as the feeding behavior.L. Reguero is in receipt of a Predoctoral Fellowship from the Basque Country Government (BFI 07.286); I. Buceta is in receipt of a Predoctoral Fellowship from the Basque Country University. Dr. Pedro Grandes' laboratory is supported by The Basque Country Government grant GIC07/70-IT-432-07, by Ministerio de Ciencia e Innovacion (SAF2009-07065) and by Red de Trastornos Adictivos, RETICS, Instituto de Salud Carlos III, MICINN, grant RD07/0001/2001. Dr. Giovanni Marsicano's laboratory is supported by AVENIR/INSERM (with the Fondation Bettencourt-Schueller), by ANR (ANR-06-NEURO-043-01), by European Foundation for the Study of Diabetes (EFSD), by the EU-FP7 (REPROBESITY, contract number HEALTH-F2-2008-223713) and European Commission Coordination Action ENINET (contract number LSHM-CT-2005-19063). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript