1,207 research outputs found
Buried and accessible surface area control intrinsic protein flexibility
Proteins experience a wide variety of conformational dynamics that can be
crucial for facilitating their diverse functions. How is the intrinsic
flexibility required for these motions encoded in their three-dimensional
structures? Here, the overall flexibility of a protein is demonstrated to be
tightly coupled to the total amount of surface area buried within its fold. A
simple proxy for this, the relative solvent accessible surface area (Arel),
therefore shows excellent agreement with independent measures of global protein
flexibility derived from various experimental and computational methods.
Application of Arel on a large scale demonstrates its utility by revealing
unique sequence and structural properties associated with intrinsic
flexibility. In particular, flexibility as measured by Arel shows little
correspondence with intrinsic disorder, but instead tends to be associated with
multiple domains and increased {\alpha}- helical structure. Furthermore, the
apparent flexibility of monomeric proteins is found to be useful for
identifying quaternary structure errors in published crystal structures. There
is also a strong tendency for the crystal structures of more flexible proteins
to be solved to lower resolutions. Finally, local solvent accessibility is
shown to be a primary determinant of local residue flexibility. Overall this
work provides both fundamental mechanistic insight into the origin of protein
flexibility and a simple, practical method for predicting flexibility from
protein structures.Comment: 36 pages, 11 figures, author's manuscript, accepted for publication
in Journal of Molecular Biolog
Distributional Impacts of Country-of-Origin Labeling in the U.S. Meat Industry
Concerns about the negative effects of U.S. meat and livestock imports on domestic livestock prices have increased interest in country-of origin labeling (COOL) legislation. An equilibrium displacement model is used to estimate short-run and long-run changes in equilibrium prices and quantities of meat and livestock in the beef, pork, and poultry sectors resulting from the implementation of COOL. Retail beef and pork demand would have to experience a one-time, permanent increase of 4.05% and 4.45%, respectively, so that feeder cattle and hog producers do not lose producer surplus over a 10-year period.country-of-origin labeling, equilibrium displacement model, producer surplus, Agribusiness,
Diverse Molecular Mechanisms Underlying Pathogenic Protein Mutations:Beyond the Loss-of-Function Paradigm
Updated benchmarking of variant effect predictors using deep mutational scanning
Abstract The assessment of variant effect predictor (VEP) performance is fraught with biases introduced by benchmarking against clinical observations. In this study, building on our previous work, we use independently generated measurements of protein function from deep mutational scanning (DMS) experiments for 26 human proteins to benchmark 55 different VEPs, while introducing minimal data circularity. Many topâperforming VEPs are unsupervised methods including EVE, DeepSequence and ESMâ1v, a protein language model that ranked first overall. However, the strong performance of recent supervised VEPs, in particular VARITY, shows that developers are taking data circularity and bias issues seriously. We also assess the performance of DMS and unsupervised VEPs for discriminating between known pathogenic and putatively benign missense variants. Our findings are mixed, demonstrating that some DMS datasets perform exceptionally at variant classification, while others are poor. Notably, we observe a striking correlation between VEP agreement with DMS data and performance in identifying clinically relevant variants, strongly supporting the validity of our rankings and the utility of DMS for independent benchmarking
Large protein complex interfaces have evolved to promote cotranslational assembly
Assembly pathways of protein complexes should be precise and efficient to minimise misfolding and unwanted interactions with other proteins in the cell. One way to achieve this efficiency is by seeding assembly pathways during translation via the cotranslational assembly of subunits. While recent evidence suggests that such cotranslational assembly is widespread, little is known about the properties of protein complexes associated with the phenomenon. Here, using a combination of proteome-specific protein complex structures and publicly available ribosome profiling data, we show that cotranslational assembly is particularly common between subunits that form large intermolecular interfaces. To test whether large interfaces have evolved to promote cotranslational assembly, as opposed to cotranslational assembly being a non-adaptive consequence of large interfaces, we compared the sizes of first and last translated interfaces of heteromeric subunits in bacterial, yeast, and human complexes. When considering all together, we observe the N-terminal interface to be larger than the C-terminal interface 54% of the time, increasing to 64% when we exclude subunits with only small interfaces, which are unlikely to cotranslationally assemble. This strongly suggests that large interfaces have evolved as a means to maximise the chance of successful cotranslational subunit binding
The properties of human disease mutations at protein interfaces
The assembly of proteins into complexes and their interactions with other biomolecules are often vital for their biological function. While it is known that mutations at protein interfaces have a high potential to be damaging and cause human genetic disease, there has been relatively little consideration for how this varies between different types of interfaces. Here we investigate the properties of human pathogenic and putatively benign missense variants at homomeric (isologous and heterologous), heteromeric, DNA, RNA and other ligand interfaces, and at different regions in proteins with respect to those interfaces. We find that different types of interfaces vary greatly in their propensity to be associated with pathogenic mutations, with homomeric heterologous and DNA interfaces being particularly enriched in disease. We also find that residues that do not directly participate in an interface, but are close in three-dimensional space, show a significant disease enrichment. Finally, we observe that mutations at different types of interfaces tend to have distinct property changes when undergoing amino acid substitutions associated with disease, and that this is linked to substantial variability in their identification by computational variant effect predictors
Ligands and receptors with broad binding capabilities have common structural characteristics:an antibiotic design perspective
Ligand Binding Site Structure Shapes Folding, Assembly and Degradation of Homomeric Protein Complexes
- âŠ