Efficacy of buffered sodium citrate alone and in combination with sodium diacetate againstListeria monocytogenes on beef franks

We assessed the antimicrobial efficacy of buffered sodium citrate alone and in combination with sodium diacetate against L. monoyctogenes on beef frank samples stored at 39°F. Initial inoculum level of L. monocytogenes was 1.5 log colony forming units (CFU)/cm2. After 6 weeks of incubation at 39°F, the pathogen reached 5.4 log CFU/cm2 in the control sample, but was 1.2 log CFU/cm2 and 0.85 log CFU/cm2 in samples treated with 1% buffered sodium citrate alone and in combination with 0.1% sodium diacetate, respectively. Use of buffered sodium citrate and the combination of buffered sodium citrate and sodium diacetate should improve safety of ready to eat foods by controlling L. monocytogenes during storage

MF2315

Melvin Hunt et al, Making ground beef patties and sausage safer, Kansas State University, January 1998

Control of Escherichia coli O157:H7 in large-diameter, lebanon-style bologna

Lebanon bologna raw batter was mixed with a five-strain mixture of Escherichia coli O157:H7 to achieve average inoculum levels of 7.79, 7.77, and 7.92 log CFU/g as deter mined on MSA, 202, and PRSA media, respectively. Treatment 1 consisted of a fermentation cycle of 8 hrs at an internal temperature (I.T.) of 80EF then 24 hrs at 100EF I.T., followed by 24 hrs at 110EF I.T. Treatments 2, 3, and 4 included additional heating at 115EF I.T. for 1, 2, and 5 hrs, respectively. All heat treatments resulted in product that was negative (\u3c1.9 log CFU/g detection limit) on all culture media and negative after enrichment on mEC selective medium. This study validates that a five-log reduction of E. coli O157:H7 can be achieved using the described protocol, thus meeting USDA/FSIS requirements

Translocation of natural microflora from muscle surface to interior by blade tenderization

The effect of blade tenderization on translocation of natural microflora from the surface to the interior of longissimus dorsi steaks aged for 7, 14, and 21 days was evaluated. Samples from the exterior and interior of steaks from blade-tenderized (BT) and non-blade-tenderized (N-BT) strip loins were analyzed for aerobic plate, coliform, and Escherichia coli counts. Results showed that BT translocated microorganisms (aerobic plate counts) from the exterior to the interior of muscle. Microorganism numbers increased with extended storage (P<.05). Counts of coliforms and Escherichia coli recovered from BT steaks were comparable to those from N-BT steaks because of very low exterior counts, showing the importance of good hygiene

Effects of quality grade, aging period, blade tenderization, and degree of doneness on tenderness of inside round steaks

We used 162 inside rounds to determine the influence of different quality grades, postmortem aging periods, blade tenderization passes, and degree of doneness on thawing and cooking losses and Warner-Bratzler Shear force (WBS, tenderness). Select (SEL), Choice (CHO), and Certified Angus Beef™ (CAB) inside rounds were aged for 7, 14, or 21 days and not tenderized (0X) or blade tenderized one (1X) or two (2X) times. Steaks from each inside round were assigned randomly to final endpoint cooking temperatures of 150, 160, and 170°F. Percentage of thawing loss was higher (P<.05) for steaks aged 7 days than steaks aged 14 and 21 days. For CHO steaks only, cooking loss was higher (P<.05) for the 2X group compared to the 0X and 1X groups. Steaks aged 14 and 21 days had lower (P<.05) WBS than steaks aged 7 days. Cooking loss and WBS were higher (P<.05) with each increase in endpoint cooking temperature. Postmortem aging (14 or 21 days) and lower endpoint cooking temperatures were the most effective methods to improve WBS of inside round steaks

Validation of a traditional Italian-style salami manufacturing process for control of salmonella and Listeria monocytogenes

Italian-style salami batter (formulated with pork shoulder) was inoculated with ca. 7.0 log CFU/g of either Salmonella or Listeria monocytogenes. Salami links (55-mm cellulose casings) were fermented at 308C for 24, 40, or 72 h and then dried to target moisture/protein ratios (MPRs) of 1.9:1 or 1.4:1. Links were sampled after fermentation (24, 40, and 72 h) and after combined fermentation-drying treatments (MPRs of 1.9:1 and 1.4:1 for all fermentation periods), and microbiological and proximate analyses were performed at each sampling. Pathogen populations were enumerated by direct plating on selective agar and by an injured-cell recovery method. When enumerated by the injured-cell recovery method, Salmonella populations were reduced by 1.2 to 2.1 log CFU/g after fermentation alone (24 to 72 h) and by 2.4 to 3.4 log CFU/g when fermentation was followed by drying. Drying to an MPR of 1.4:1 was no more effective than drying to an MPR of 1.9:1 (P . 0.05). When enumerated directly on selective media, Salmonella populations were reduced from 1.6 to 2.4 log CFU/g and from 3.6 to 4.5 log CFU/g for fermentation alone and fermentation followed by drying, respectively. L. monocytogenes populations were reduced by ,1.0 log CFU/g following all fermentation and combined fermentation-drying treatments, regardless of the enumeration method. These results suggest that the Italian-style salami manufacturing process evaluated does not adequately reduce high pathogen loads. Processors may thus need to consider supplemental measures, such as raw material specifications and a final heating step, to enhance the lethality of the overall manufacturing process

Effects of injection marination with various calcium sources and molar concentrations on display color life, tenderness, and microbial inhibition of beef loin steaks

Beef strip loins were assigned to one of 11 treatments that included injection marination (10% by weight) with three calcium salts at three molar concentrations, a distilled water control, and a non-marinated control. The effects of calcium salt and concentration were tested for retail display color life, tenderness and sensory traits, and microbial growth. Calcium lactate marinated steaks had longer color life and less microbial growth than those treated with calcium chloride or calcium ascorbate. Increasing molar concentration (.1M to .2M to .3M) caused faster color deterioration, and did not significantly improve microbial inhibition. All calcium treatments improved tenderness; however, calcium chloride treatments induced off-flavors. Considering a whole system approach that accounts for color life, microbial inhibition, shear force, and sensory traits, we recommend injecting beef longissimus with 10% of a .1M solution of calcium lactate, and do not recommend other calcium salts or concentrations

Effects of cetylpyridinium chloride treatment of roast beef on Listeria monocytogenes populations and quality attributes

The effectiveness of cetylpyridinium chloride (CPC) for reducing microbial populations, in particular Listeria monocytogenes, on ready-to-eat roast beef was evaluated. Roast beef slices inoculated with L. monocytogenes were dipped in a solution of 1% CPC for 1 minute. Samples were then vacuum packaged and stored at refrigeration temperature. The effects of CPC treatment on microbial populations, as well as on color and texture of the roast beef samples, was evaluated over a 42-day period. Immediately after CPC treatment, L. monocytogenes populations were reduced by 99 to 99.99%, with the treatment being somewhat more effective on exterior than on sliced/cut surfaces. Throughout 42 days of refrigerated storage, populations of L. monocytogenes, total bacteria, and lactic acid bacteria remained lower on CPC-treated samples than on non-treated samples. Treatment with CPC did not significantly affect the color or texture of roast beef. Treatment with CPC, especially when applied to products before slicing, may serve as an effective antimicrobial intervention for ready-to-eat meat products

Control of Escherichia coli O157:H7 in large-diameter, lebanon-style bologna

Lebanon bologna raw batter was mixed with a five-strain mixture of Escherichia coli O157:H7 to achieve average inoculum levels of 7.79, 7.77, and 7.92 log CFU/g as deter mined on MSA, 202, and PRSA media, respectively. Treatment 1 consisted of a fermentation cycle of 8 hrs at an internal temperature (I.T.) of 80EF then 24 hrs at 100EF I.T., followed by 24 hrs at 110EF I.T. Treatments 2, 3, and 4 included additional heating at 115EF I.T. for 1, 2, and 5 hrs, respectively. All heat treatments resulted in product that was negative (<1.9 log CFU/g detection limit) on all culture media and negative after enrichment on mEC selective medium. This study validates that a five-log reduction of E. coli O157:H7 can be achieved using the described protocol, thus meeting USDA/FSIS requirements

Sensory traits, color, and shelf life of low-dose irradiated, raw, ground beef patties

Irradiation of raw ground beef patties had minimal effects on flavor and aroma of patties after cooking. Oxidative rancidity increased when patties were irradiated in aerobic but not in vacuum packages. Irradiation of vacuum-packaged ground beef patties produced a more stable color. In both packaging types, irradiation significantly reduced microbial growth during storage