Seasonal Occurrence and Distribution on Grapevine Roots of Eurhizococcus brasiliensis (Wille) (Hemiptera: Margarodidae) in Brazil

The ground pearl, Eurhizococcus brasiliensis (Wille) (Hemiptera: Margarodidae), is the most importantgrapevine pest in Brazil. Its seasonal occurrence and distribution on the roots of the different developmentstages were determined to allow better monitoring of this insect and better targeting of its vulnerable lifestages. Yellow cysts (after the first nymphal moult) showed the lowest density in October, followed by agradual increase towards August. White cysts (cysts with enclosed pre-pupal males or females) occurredfrom August to December, with a peak in November. Mobile females (adult females emerging from thewhite cysts) were found from August to December, with a peak in August. Parthenogenetic females thatremain in the ruptured white cysts for egg laying were present from August to April, with a peak inNovember. Mobile nymphs (first instar) were also found from August to April, with a peak in December.Yellow cysts were most abundant at depths of 0 to 25 cm. The horizontal survey showed that cysts occurredmostly on the trunk below the ground (trunk of the rootstock), and that almost all occurred in an area of20 cm width around the trunk. These results provide important information for better monitoring of thispest and to develop better methods for and timing of chemical control

Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013-2014

Published online feb. 2016. Ssubject to Restrictions below, author can archive publisher's version/PDF Restrictions: - 12 months embargoSubmitted by Sandra Infurna ([email protected]) on 2017-06-23T18:59:55Z No. of bitstreams: 1 elbaregina2_lemos_etal_IOC_2016.pdf: 686130 bytes, checksum: c574717046ce1ab30920168f612c53f6 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-06-23T19:16:23Z (GMT) No. of bitstreams: 1 elbaregina2_lemos_etal_IOC_2016.pdf: 686130 bytes, checksum: c574717046ce1ab30920168f612c53f6 (MD5)Made available in DSpace on 2017-06-23T19:16:23Z (GMT). No. of bitstreams: 1 elbaregina2_lemos_etal_IOC_2016.pdf: 686130 bytes, checksum: c574717046ce1ab30920168f612c53f6 (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Hospital Municipal Desembargador Leal Junior. Laboratório de Análises Clínicas. Rio de Janeiro, RJ, Brasil.Hospital Municipal Desembargador Leal Junior. Laboratório de Análises Clínicas. Rio de Janeiro, RJ, Brasil.Hospital Municipal Desembargador Leal Junior. Laboratório de Análises Clínicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnológica em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Q fever is an important cause of undifferentiated fever that is rarely recognized or reported in Brazil. The objective of this study was to look for the presence of Coxiella burnetii during a dengue fever outbreak in the municipality of Itaboraí, Rio de Janeiro, Brazil, where this bacterium had previously infected humans and domesticated animals. Blood samples from clinically suspected dengue fever patients were tested by polymerase chain reaction (PCR) for C. burnetii; the DNA was detected in nine (3.3%) of 272 patients. One was coinfected with dengue virus, which was also detected in another 166 (61.3%) patients. The nucleotide sequence of PCR amplification and DNA sequencing of the IS1111 transposase elements in the genome of C. burnetii exhibited 99% identity with the sequence in GenBank. The detection of C. burnetii in patients suspected of dengue fever indicates that awareness and knowledge of Q fever should be strengthened and that this bacterium is present in Brazil. Finally, because a negative molecular result does not completely rule out the diagnosis of Q fever and the serological assay based on seroconversion was not available, the actual number of this zoonosis is likely to be much higher than that reported in this study