The landscape of BRAF transcript and protein variants in human cancer

Background: The BRAF protein kinase is widely studied as a cancer driver and therapeutic target. However, the regulation of its expression is not completely understood. Results: Taking advantage of the RNA-seq data of more than 4800 patients belonging to 9 different cancer types, we show that BRAF mRNA exists as a pool of 3 isoforms (reference BRAF, BRAF-X1, and BRAF-X2) that differ in the last part of their coding sequences, as well as in the length (BRAF-ref: 76 nt; BRAF-X1 and BRAF-X2: up to 7 kb) and in the sequence of their 3'UTRs. The expression levels of BRAF-ref and BRAF-X1/X2 are inversely correlated, while the most prevalent among the three isoforms varies from cancer type to cancer type. In melanoma cells, the X1 isoform is expressed at the highest level in both therapy-naïve cells and cells with acquired resistance to vemurafenib driven by BRAF gene amplification or expression of the Δ[3-10] splicing variant. In addition to the BRAF-ref protein, the BRAF-X1 protein (the full length as well as the Δ[3-10] variant) is also translated. The expression levels of the BRAF-ref and BRAF-X1 proteins are similar, and together they account for BRAF functional activities. In contrast, the endogenous BRAF-X2 protein is hard to detect because the C-terminal domain is selectively recognized by the ubiquitin-proteasome pathway and targeted for degradation. Conclusions: By shedding light on the repertoire of BRAF mRNA and protein variants, and on the complex regulation of their expression, our work paves the way to a deeper understanding of a crucially important player in human cancer and to a more informed development of new therapeutic strategies

Framing alleged Islamist plots: a case study of British press coverage since 9/11

In the decade post 9/11 , the UK terrorist threat was associated with a series of high profile counter terrorism operations, linked to specific plots. These terrorism related episodes received significant media attention and, as a consequence, were a visible sign of the contemporary terrorist threat. This paper seeks to identify the dominant frames rendered in news media reporting on these episodes. Through a longitudinal study of UK press coverage, the analysis reveals that two prominent frames were present, an inevitability and preparedness frame, with alleged plots serving to underline the risk posed by contemporary terrorism,and a belonging and responsibility frame, which cast later episodes as belonging to the Muslim communities disrupted by polic

Crafting History: How the World Is Made. The Case of Islamic Archaeology

In this paper an archaeological and theoretical perspective that builds a relationship between the concepts of craft and of identity is presented. Both of them are concepts very widely used in archaeological and anthropological theory nowadays, and they have often been linked in field studies. However, these concepts are usually contemplated from very different points of view and with many diverse implications in each case. One of the aims of this paper is to show that craft and identity can be inserted in a common theoretical framework which in turn can be used to understand cultural change or, in other words, history within culture. The paper will start with a necessary theoretical introduction to different concepts related to craft and identity, and then a discussion on how to link these different concepts will follow. In the last part of the paper, this theoretical perspective will be applied to a field which is familiar to the author, that of Islamic archaeology. A case example of the author’s research in the Vega of Granada (southeast Spain) will be brought to the fore. This part of the paper will show how the theoretical discussion developed above can contribute to solve one of the core questions of this field, that of the definition of an Islamic culture and its application to understand the daily life of people living within it

Sociology and Anthropology of Islam a Critical Debate

10.1002/9781444320787.ch16The New Blackwell Companion to the Sociology of Religion364-38

Defensive or offensive dining? Halal dining practices among Malay Muslim Singaporeans and their effects on integration

10.1111/j.1757-6547.2012.00166.xAustralian Journal of Anthropology (The)23184-10

Integration, minorities and the rhetoric of civilization: The case of British Pakistani Muslims in the UK and Malay Muslims in Singapore

10.1080/01419870.2010.539702Ethnic and Racial Studies345814-83

The Adhan among the Bels : studying Muslim identity in Northern Ireland

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Studying Muslims of Europe

10.1002/9781118257203.ch17A Companion to the Anthropology of Europe295-30