WIRIS OM tools: a semantic formula editor

With the increasing reliance on computers for the automatic processing of information a new method is needed for editing mathematical formulae. We are used to WYSIWYG editors that produce beautiful presentations of formulae and store the typesetting primitives rather than the meaning of the formulas. However, new services such as database searching or calculation web-services work best if they have access to the semantic information behind a formula. This can only be done with a new generation of formula editors. In this paper we present WIRIS OM Tools [17], a semantic oriented formula editor which addresses these concerns. It is based on the OpenMath language and a suitable transformation process between OpenMath and MathML ex- pressions. Additionally, this approach adds new features for the users such as error, type and syntax checking. The editor is currently being used in the LeActiveMath and WebALT projects

Seismic diagnostics for transport of angular momentum in stars 2. Interpreting observed rotational splittings of slowly-rotating red giant stars

Asteroseismology with the space-borne missions CoRoT and Kepler provides a powerful mean of testing the modeling of transport processes in stars. Rotational splittings are currently measured for a large number of red giant stars and can provide stringent constraints on the rotation profiles. The aim of this paper is to obtain a theoretical framework for understanding the properties of the observed rotational splittings of red giant stars with slowly rotating cores. This allows us to establish appropriate seismic diagnostics for rotation of these evolved stars. Rotational splittings for stochastically excited dipolar modes are computed adopting a first-order perturbative approach for two $1.3 M_\odot$ benchmark models assuming slowly rotating cores. For red giant stars with slowly rotating cores, we show that the variation of the rotational splittings of $\ell=1$ modes with frequency depends only on the large frequency separation, the g-mode period spacing, and the ratio of the average envelope to core rotation rates (${\cal R}$). This leds us to propose a way to infer directly ${\cal R}$ from the observations. This method is validated using the Kepler red giant star KIC 5356201. Finally, we provide a theoretical support for the use of a Lorentzian profile to measure the observed splittings for red giant stars.Comment: 15 pages, 15 figures, accepted for publication in A&

Ganho de peso bovino em resteva de arroz irrigado na safra de 2009/2010 na Embrapa Pecuária Sul.

bitstream/item/31962/1/DT-102online.pd

Development and validation of a multiplex-PCR assay for X-linked intellectual disability

BACKGROUND: X-linked intellectual disability is a common cause of inherited cognitive deficit affecting mostly males. There are several genetic causes implicated in this condition, which has hampered the establishment of an accurate diagnosis. We developed a multiplex-PCR assay for the mutational hotspot regions of the FMR1, AFF2 and ARX genes. METHODS: The multiplex-PCR was validated in a cohort of 100 males selected to include known alleles for the FMR1 repetitive region: five full mutations (250-650 CGGs), ten premutations (70-165 CGGs) and eighty-five in the normal range (19-42 CGGs). Sequencing or Southern blotting was used to confirm the results, depending on the allele class. In this cohort, with the exception of one sample showing an AFF2 intermediate-sized allele, all other samples were normal (8-34 CCGs). No ARX variant was found besides the c.429_452dup. The validated assay was applied to 5000 samples (64.4% males and 35.6% females). RESULTS: The normal-allelic range of both FMR1 and AFF2 genes as well as the nature of ARX variants identified was similar in both genders. The rate of homozygosity observed in female samples, 27.5% for FMR1 and 17.8% for AFF2 alleles, is comparable to that published by others. Two FMR1 premutations were identified, in a male (58 CGGs) and a female case [(CGG)(47)/(CGG)(61)], as well as several FMR1 or AFF2 intermediate-sized alleles. One AFF2 premutation (68 CCGs) and two putative full expansions were picked up in male subjects, which seems relevant considering the rarity of reported AFF2 mutations found in the absence of a family history. CONCLUSIONS: We developed a robust multiplex-PCR that can be used to screen the mutational hotspot regions of FMR1, AFF2 and ARX genes. Moreover, this strategy led to the identification of variants in all three genes, representing not only an improvement in allele-sizing but also in achieving a differential diagnosis. Although the distinction between females who are truly homozygous and those with a second pre- or full mutation sized allele, as well as a definitive diagnosis, requires a specific downstream technique, the use of this multiplex-PCR for initial screening is a cost-effective approach which widens the scope of detection

Analysis of the economic viability from an integrated system of rice and beef cattle in the Pampa Biome of Rio Grande do Sul.

The environmental conditions of the Pampa Biome are favorable to the degradation of soil, especially when grain crops are introduced in this region, traditionally exploited with beef cattle systems