The non-coding RNA landscape of human hematopoiesis and leukemia

© The Author(s) 2017. Non-coding RNAs have emerged as crucial regulators of gene expression and cell fate decisions. However, their expression patterns and regulatory functions during normal and malignant human hematopoiesis are incompletely understood. Here we present a comprehensive resource defining the non-coding RNA landscape of the human hematopoietic system. Based on highly specific non-coding RNA expression portraits per blood cell population, we identify unique fingerprint non-coding RNAs-such as LINC00173 in granulocytes-and assign these to critical regulatory circuits involved in blood homeostasis. Following the incorporation of acute myeloid leukemia samples into the landscape, we further uncover prognostically relevant non-coding RNA stem cell signatures shared between acute myeloid leukemia blasts and healthy hematopoietic stem cells. Our findings highlight the importance of the non-coding transcriptome in the formation and maintenance of the human blood hierarchy

МЕТОД АТОМНО-СИЛОВОЙ МИКРОСКОПИИ В ИССЛЕДОВАНИИ ТРОМБОЦИТОВ ПАЦИЕНТОВ С ТЕРМИНАЛЬНОЙ СТАДИЕЙ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ

Atomic force microscopy was used to estimate the intravascular platelet activation and determine local elastic modulus, adhesion and stiffness of the platelets membrane in patients with end-stage of chronic heart failure with heart ventricle implantation before surgery and intrasurgical: before the heparin injection, after the heparin injection and after neutralization of heparin with protamine.Методом атомно-силовой микроскопии производилась оценка внутрисосудистой активации тромбоцитов и определялись локальный модуль упругости, адгезия, жесткость мембраны тромбоцитов у пациентов с терминальной стадией хронической сердечной недостаточности с использованием имплантируемого двухжелудочкового вспомогательного сердца на этапах до операции и интраоперационно: до введения гепарина, после введения гепарина, после нейтрализации гепарина протамином

Application of atomic force microscopy to evaluate the morphofunctional state of platelets in patients with end-stage heart failure

В работе методом АСМ определялись морфометрические характеристики тромбоцитов и тромбоцитарных агрегатов, а также локальные вязко-упругие характеристики мембраны кровяных пластинок (локальный модуль упругости, адгезия, жесткость) пациентов с терминальной стадией хронической сердечной недостаточности.In the study, the morphometric characteristics of platelets and platelet aggregates, as well as local viscoelastic characteristics of platelet membrane (local modulus of elasticity, adhesion, stiffness) of patients with end-stage heart failure were investigated by atomic force microscopy

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Аналитические методы оценки интеллектуального капитала / M. О. Мороз, Ю. А. Мышковец

The article discusses the use of various methods of evaluating the intellectual capital of companies, provides a comparative description of their main advantages and disadvantages, as well as recommendations on their applying for the most efficient use of intellectual capital in order to achieve strategic goals and increase the value of companies.В статье рассматриваются вопросы применения различных методов оценки интеллектуального капитала компаний, дается сравнительная характеристика их основных преимуществ и недостатков, а также рекомендации по их применению для наиболее эффективного использования интеллектуального капитала для достижения стратегических целей и повышения стоимости компаний

Аналитические методы оценки интеллектуального капитала / M. О. Мороз, Ю. А. Мышковец

The article discusses the use of various methods of evaluating the intellectual capital of companies, provides a comparative description of their main advantages and disadvantages, as well as recommendations on their applying for the most efficient use of intellectual capital in order to achieve strategic goals and increase the value of companies.В статье рассматриваются вопросы применения различных методов оценки интеллектуального капитала компаний, дается сравнительная характеристика их основных преимуществ и недостатков, а также рекомендации по их применению для наиболее эффективного использования интеллектуального капитала для достижения стратегических целей и повышения стоимости компаний