7 research outputs found

    Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

    Get PDF
    A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity

    Economic impacts of yam productivity research in West Africa: A case of YIIFSWA Project

    No full text
    A bold step to addressing myriads of constraints affecting yam productivity was achieved through Yam for Income and Food Security in West Africa (YIIFSWA) project. The project has embarked on a series of activities culminating in the development, deployment and disseminating intervention options/technologies in Nigeria and Ghana. This paper assesses the potential economic impacts, the number of beneficiaries and poverty reduction through these agricultural technologies/intervention options. The land area coverable by the technologies ranged 320,000–650,000 ha in the two countries. The land area under varieties for adaptation to environments with low soil fertility was the highest followed by resistance to nematode cultivars. The net present value (NPV) ranged 144million–144 million–616 million and was highest for YIIFSWA diagnostic tool and temporary immersion bioreactor. Crop management and postharvest practices option had the lowest benefit-cost ratios of 6.0 and 20.03 while the aeroponics option had the highest benefit cost ratio of about 36.90. Not less than 750, 000 would be brought out of poverty by these technologies. The technologies are expected to reach not less than 20 million households by 2037 in Nigeria and Ghana. The technologies are more responsive to change in adoption rate than change in costs. Overall, while the potential economic gains are considerable, realization of these gains depends on the efficiency and effectiveness of extension and input supply systems. Concerted extension efforts are needed to drive the use of these intervention options. Moreover, considerable technical advice would also be needed to explain how to apply the

    Genotype × Environment Interaction of Mosaic Disease, Root Yields and Total Carotene Concentration of Yellow-Fleshed Cassava in Nigeria

    Get PDF
    Twenty-one yellow-fleshed cassava genotypes were evaluated over two years in five major cassava growing agroecological zones in Nigeria. The trials were established in a randomized complete block design with four replications to assess genotype performance and Genotype × Environment interaction for cassava mosaic disease (CMD), fresh and dry root yield (FYLD; DYLD), root dry matter content (DMC), and total carotene concentration (TCC). Combined analysis of variance showed significant differences (P<0.001) among genotypes (G), environment (E), and Genotype × Environment interaction (GE) for all the traits tested. For reaction to CMD, the best genotypes showing stable resistance were TMS 07/0539 and TMS 07/0628. For root yield, the best genotypes were TMS 01/1368 and TMS 07/0553. Genotype TMS 07/0593 was the best for DMC and TCC across the 10 environments. Variation among genotypes accounted for most of the Total Sum of Squares for CMD (72.1%) and TCC (34.4%). Environmental variation accounted for most of the Total Sum of Squares for FYLD (42.8%), DYLD (39.6%), and DMC (29.2%). This study revealed that TMS 07/0593 has the highest and most stable TCC, DMC with the lowest CMD severity score and appeared to be the best genotype

    Environment Interaction of Mosaic Disease, Root Yields and Total Carotene Concentration of Yellow-Fleshed Cassava in Nigeria

    No full text
    Twenty-one yellow-fleshed cassava genotypes were evaluated over two years in five major cassava growing agroecological zones in Nigeria. The trials were established in a randomized complete block design with four replications to assess genotype performance and Genotype × Environment interaction for cassava mosaic disease (CMD), fresh and dry root yield (FYLD; DYLD), root dry matter content (DMC), and total carotene concentration (TCC). Combined analysis of variance showed significant differences (P &lt; 0.001) among genotypes (G), environment (E), and Genotype × Environment interaction (GE) for all the traits tested. For reaction to CMD, the best genotypes showing stable resistance were TMS 07/0539 and TMS 07/0628. For root yield, the best genotypes were TMS 01/1368 and TMS 07/0553. Genotype TMS 07/0593 was the best for DMC and TCC across the 10 environments. Variation among genotypes accounted for most of the Total Sum of Squares for CMD (72.1%) and TCC (34.4%). Environmental variation accounted for most of the Total Sum of Squares for FYLD (42.8%), DYLD (39.6%), and DMC (29.2%). This study revealed that TMS 07/0593 has the highest and most stable TCC, DMC with the lowest CMD severity score and appeared to be the best genotype

    Yam seed system characteristics in Nigeria : Local practices, preferences, and the implications for seed system interventions

    No full text
    Yam is an important food and cash crop in Nigeria. The seed system is mostly farmer-based and its challenges and opportunities are not well documented. This study reports results of surveys, interviews, focus group discussions and participant observations that describe local practices characterizing yam cultivation and the farmer-based seed system in five states in Nigeria. Our findings show that ware and seed yam are dynamic and highly commercialized goods in Nigeria. There is a high demand for, and a high turn-over of, seed yam. The reasons for the high demand are the low multiplication rate and degeneration of seed yam which lead farmers to frequently acquire fresh seeds and replace varieties, investing significantly in the seed of ware yam. The study found some farmers specializing in seed yam production but less than expected for such a highly commercial crop. The market is the major source for off-farm sourced seed yam, and although completely informal, the seed yam sector is vibrant and well organized. The identification of strategically positioned farmers and traders can offer opportunities and entry points for introduction of new varieties and improved seed production techniques. Building on the existing farmer-based system is a more logical strategy than replacing it with formal structures and legislative regulation
    corecore