Appendix B. A description of the statistical analyses and tests for spatial autocorrelation.

A description of the statistical analyses and tests for spatial autocorrelation

Blackburn&Rodel_Phrynobatrachus_schioetzi

Nexus file containing aligned mtDNA (12S, tRNA-Valine, 16S) for species of Phrynobatrachus

Study ponds assigned according to the results of the k-means clustering.

<p>Ponds are plotted in the first two principal components using 16 abiotic habitat characteristics, explaining 40.53 % of data variance. Ponds with emigrating <i>Rana temporaria</i> metamorphs are indicated by filled, non-emigration ponds by open triangles. Loadings of the components are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089982#pone.0089982.s003" target="_blank">Table S3</a>.</p

Pond characteristics and metamorphic traits.

<p>Visualization of environmental parameters in relation to <i>Rana temporaria</i> metamorphic mass (median) and development time (median). Values are colour-coded, warm colours indicating higher values. Ponds (bottom) are ordered according to their values for the respective trait, the values for the respective environmental parameter are given in the column above. There is no correlation between traits and environmental parameter visible (rows).</p

LCP distance data

Landscape distances and variables included in each least-cost path (LCP) between study sites of Amietia wittei and Amietia angolensis calculated in ArcGIS 10 (ESRI). For each LCP model between pairs of sites (rows) the following variables (columns) were calculated: topography-corrected route lengths (distance); weighted-average values of compound topographic index (cti), roughness (rough) and slope; proportions of cropland, homegarden, human settlements, erica shrubs, forest, disturbed forest, reforestation and grassland. A 30 x 30 m digital elevation model (DEM) produced by J.A. Ong'injo, C. Lambrechts and A. Help was use for calculating resistance surfaces. Stream network was delineated using the "flow accumulation" tool in ArcGIS 10 (ESRI). The land cover surface was modified from the original produced by T. Appelhans and T. Nauss (unpubl.). Straight-line distances (Euclidean) are also reported and used as null models

Summary of metamorphing <i>Rana temporaria</i> juveniles and predator- and tadpole-density in the respective ponds in 2007.

<p>Survival rate was based on an average of 1117 eggs per clutch. For metamorphic traits, mean, coefficient of variance (CV), as well as minimum and maximum size and mass of individuals for each pond is given. For predator- and tadpole-density, mean and SD of all samplings, number of sampling events and total number of box samplings summed up for realized samplings are given (see methods for detailed description). For pond abbreviations, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089982#pone.0089982-Grzinger1" target="_blank">[31]</a>.</p><p>cl.  = clutches; meas.  = measured, devel.time = development time, samp.  = samplings: events/total samplings.</p