Reduction of Acid-Fast and Non-Acid-Fast Bacteria by Point of Use Coagulation-Flocculation-Disinfection

Point of use (POU) household water treatment is increasingly being adopted as a solution for access to safe water. Non-tuberculous Mycobacteria (NTM) are found in water, but there is little research on whether NTM survive POU treatment. Mycobacteria may be removed by multi-barrier treatment systems that combine processes such as coagulation, settling and disinfection. This work evaluated removal of a non-tuberculous Mycobacterium (Mycobaterium terrae) and a Gram-negative non-acid-fast environmental bacterium (Aeromonas hydrophila) by combined coagulation-flocculation disinfection POU treatment. Aeromonas hydrophila showed 7.7 log10 reduction in demand free buffer, 6.8 log10 in natural surface water, and 4 log10 reduction in fecally contaminated surface water. Turbidity after treatment wasreductions

Local drinking water filters reduce diarrheal disease in Cambodia: a randomized, controlled trial of the ceramic water purifier.

A randomized, controlled intervention trial of two household-scale drinking water filters was conducted in a rural village in Cambodia. After collecting four weeks of baseline data on household water quality, diarrheal disease, and other data related to water use and handling practices, households were randomly assigned to one of three groups of 60 households: those receiving a ceramic water purifier (CWP), those receiving a second filter employing an iron-rich ceramic (CWP-Fe), and a control group receiving no intervention. Households were followed for 18 weeks post-baseline with biweekly follow-up. Households using either filter reported significantly less diarrheal disease during the study compared with a control group of households without filters as indicated by longitudinal prevalence ratios CWP: 0.51 (95% confidence interval [CI]: 0.41-0.63); CWP-Fe: 0.58 (95% CI: 0.47-0.71), an effect that was observed in all age groups and both sexes after controlling for clustering within households and within individuals over time

Improving Household Drinking Water Quality: Use of Biosand Filter in Cambodia

The BSF is a robust water treatment technology for use in rural Cambodian households, capable of effective removal of bacteria, and significant reduction of diarrheal disease. BSF performance is comparable to other recommended household water treatment interventions

The compartment bag test (CBT) for enumerating fecal indicator bacteria: Basis for design and interpretation of results

For the past several years, the compartment bag test (CBT) has been employed in water quality monitoring and public health protection around the world. To date, however, the statistical basis for the design and recommended procedures for enumerating fecal indicator bacteria (FIB) concentrations from CBT results have not been formally documented. Here, we provide that documentation following protocols for communicating the evolution of similar water quality testing procedures. We begin with an overview of the statistical theory behind the CBT, followed by a description of how that theory was applied to determine an optimal CBT design. We then provide recommendations for interpreting CBT results, including procedures for estimating quantiles of the FIB concentration probability distribution, and the confidence of compliance with recognized water quality guidelines. We synthesize these values in custom user-oriented 'look-up' tables similar to those developed for other FIB water quality testing methods. Modified versions of our tables are currently distributed commercially as part of the CBT testing kit

Assessing the Microbial Quality of “Improved’ Drinking Water Sources: Results from the Dominican Republic.

Millennium Development Goal Target 7c (to halve between 1990 and 2015 the proportion of the global population without sustainable access to safe drinking water), was celebrated as achieved in 2012. However, new studies show that we may be prematurely celebrating. Access to safe drinking water may be overestimated if microbial water quality is considered. The objective of this study was to examine the relationship between microbial drinking water quality and drinking water source in the Puerto Plata region of the Dominican Republic. This study analyzed microbial drinking water quality data from 409 households in 33 communities. Results showed that 47% of improved drinking water sources were of high to very-high risk water quality, and therefore unsafe for drinking. This study provides evidence that the current estimate of safe water access may be overly optimistic, and microbial water quality data are needed to reliably assess the safety of drinking water

A Randomized Controlled Trial of the Concrete Biosand Filter and its Impact on Diarrheal Disease in Bonao, Dominican Republic.

A number of household water treatment and safe storage technologies, such as chlorine disinfection, solar disinfection, and ceramic filtration, have been documented for their ability to reduce diarrheal disease and improve microbial water quality. The biosand filter (BSF) is a promising household water treatment technology in use by \u3e 500,000 people globally. The purpose of this research was to document the ability of BSFs to improve water quality and to reduce diarrheal disease in user compared with non-user households in a randomized controlled trial in Bonao, Dominican Republic, during 2005–2006. During the 6-month intervention period, 75 BSF households had significantly improved drinking water quality on average compared with 79 control households ( P \u3c 0.001). Based on random intercepts logistic regression, BSF households had 0.53 times the odds of diarrheal disease as control households, indicating a significant protective effect of the BSF against waterborne diarrheal diseas

The operation, flow conditions and microbial reductions of an intermittently operated, household-scale slow sand filter

Nearly one-fifth of the world\u27s population lacks access to safe, reliable sources of drinking water. Point of use (POU) household water treatment technology allows people to improve the quality of their water by treating it in the home. A promising emerging POU technology is the biosand filter (BSF). The BSF is a household-scale, intermittently operated slow sand filter that maintains a wet media bed containing a schmutzdecke and allows periodic water dosing by the user. Step input chemical tracer tests indicated that the BSF operates at near-plug flow conditions. Six-to-eight week longitudinal challenge studies were conducted with daily charges of surface water spiked with E. coli strain B bacteria, coliphages MS2 and PRD-1 and human enteric virus echovirus type 12. The BSF ripened in a manner similar to conventional SSFs. Flow rate slowed and microbial reductions improved over time with ripening. E. coli reductions were ~90% following filter startup but improved to 95—99.5% over time. Microbial reductions were greater with greater residence time within the filter, especially for water retained in the filter bed overnight. E. coli and echovirus 12 reductions were greater than those of coliphages MS2 and PRD-1