22 research outputs found
Data generated by quantitative LC-MS proteomics are only the start and not the endpoint: Optimization of QconCAT-based measurement of hepatic UDP-glucuronosyltransferase enzymes with reference to catalytic activity
No full textQuantitative Characterization of Major Hepatic UDP-Glucuronosyltransferase Enzymes in Human Liver Microsomes:Comparison of Two Proteomic Methods and Correlation with Catalytic Activity
Get PDFQuantitative Characterization of Major Hepatic UDP-Glucuronosyltransferase Enzymes in Human Liver Microsomes:Comparison of Two Proteomic Methods and Correlation with Catalytic Activity
No full textCross-laboratory evaluation of UDP-glucuronosyltransferase (UGT) abundances and correlation with catalytic activity
No full textMiR-409-3p regulates angiogenesis, white to brown adipose tissue transition, and insulin resistance through MAP4K3 and ZEB1 signaling pathways.
No full textEndothelial cells (ECs) within the microvasculature of brown adipose tissue (BAT) are important in regulating the plasticity of adipocytes in response to increased metabolic demand by modulating the angiogenic response. However, the mechanism of EC-adipocyte crosstalk during this process is not completely understood. We used RNA sequencing to profile microRNAs derived from BAT ECs of obese mice and identified an anti-angiogenic microRNA, miR-409-3p. MiR-409-3p overexpres- sion inhibited EC angiogenic properties; whereas, its inhibition had the opposite effects. Mechanistic studies revealed that miR-409-3p targets ZEB1 and MAP4K3. Knockdown of ZEB1/MAP4K3 phenocopied the angiogenic effects of miR-409-3p. Adipocytes co-cultured with conditioned media from ECs deficient in miR-409-3p showed increased expression of BAT markers, UCP1 and CIDEA. We identified a pro-angiogenic growth factor, placental growth factor (PLGF), released from ECs in response to miR-409-3p inhibition. Deficiency of ZEB1 or MAP4K3 blocked the release of PLGF from ECs and PLGF stimulation of 3T3-L1 adipocytes increased UCP1 expression in a miR-409-3p dependent manner. MiR-409-3p neutraliza- tion improved BAT angiogenesis, glucose and insulin tolerance, and energy expenditure in mice with diet-induced obesity. These findings establish miR-409-3p as a critical regulator of EC-BAT crosstalk by modulating a ZEB1-MAP4K3-PLGF signaling axis, providing new insights for therapeutic intervention in obesity
Data Generated by Quantitative Liquid Chromatography-Mass Spectrometry Proteomics Are Only the Start and Not the Endpoint: Optimization of Quantitative Concatemer-Based Measurement of Hepatic Uridine-5âČ-DiphosphateâGlucuronosyltransferase Enzymes with Reference to Catalytic Activity
No full textLa forĂȘt
No full textCollection : RadiovisionRĂ©sumĂ© : Recouvrant un cinquiĂšme du territoire français, la forĂȘt est composĂ©e de diffĂ©rentes espĂšces d'arbre. Le chĂȘne, arbre le plus noble et le plus prĂ©cieux qui peut vivre jusqu'Ă 800 ans, le hĂȘtre et encore l'Ă©picĂ©a en montagne. La maniĂšre de cultiver la forĂȘt est Ă repenser depuis que le bois n'est plus utilisĂ© comme combustible. (source : RĂ©seau CanopĂ©)DurĂ©e : 00:29:20ThĂšme : Sciences de la natur
