Animal waste management

"71/1M""Livestock producers have asked for guidelines on animal waste management that will be feasible and enduring. The Missouri Water Pollution Board has been aware of the need for improvements in methods of handling waste from confined feeding operations and for guidelines for producers. Chapter 204 of Missouri Statutes, as amended, gives the Water Pollution Board the responsibility and authority to correct and/or prevent "pollution" of "waters of the state." These terms are defined in the law and discussed briefly in the first section. With these facts in mind, staff engineers of the Water Pollution Board held a series of meetings with staff members of the Extension Division and Department of Agricultural Engineering of the University of Missouri-Columbia to develop guidelines for disposing of waste from confinement feeding operations. This report is a result of their combined efforts. Others assisting with various phases of development of these guidelines included: School of Engineering, University of Missouri-Columbia; State Department of Health, and the Soil Conservation Service. Research data and experience in handling livestock wastes have been used to develop the guidelines for planning, design, construction, and management of alternative systems of livestock waste management. The information and design guidelines herein are intended primarily for the use of personnel in agencies concerned with animal waste management problems." --PrefaceMissouri Water Pollution Board and Extension Division, University of Missouri - Columbia

Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation

<p>Abstract</p> <p>Background</p> <p>Intestinal barrier function is important for preserving health, as a compromised barrier allows antigen entry and can induce inflammatory diseases. Probiotic bacteria can play a role in enhancing intestinal barrier function; however, the mechanisms are not fully understood. Existing studies have focused on the ability of probiotics to prevent alterations to tight junctions in disease models, and have been restricted to a few tight junction bridging proteins. No studies have previously investigated the effect of probiotic bacteria on healthy intestinal epithelial cell genes involved in the whole tight junction signalling pathway, including those encoding for bridging, plaque and dual location tight junction proteins. Alteration of tight junction signalling in healthy humans is a potential mechanism that could lead to the strengthening of the intestinal barrier, resulting in limiting the ability of antigens to enter the body and potentially triggering undesirable immune responses.</p> <p>Results</p> <p>The effect of <it>Lactobacillus plantarum </it>MB452 on tight junction integrity was determined by measuring trans-epithelial electrical resistance (TEER) across Caco-2 cell layers. <it>L. plantarum </it>MB452 caused a dose-dependent TEER increase across Caco-2 cell monolayers compared to control medium. Gene expression was compared in Caco-2 cells untreated or treated with <it>L. plantarum </it>MB452 for 10 hours. Caco-2 cell RNA was hybridised to human oligonucleotide arrays. Data was analysed using linear models and differently expressed genes were examined using pathway analysis tools. Nineteen tight junction-related genes had altered expression levels in response to <it>L. plantarum </it>MB452 (modified-P < 0.05, fold-change > 1.2), including those encoding occludin and its associated plaque proteins that anchor it to the cytoskeleton. <it>L. plantarum </it>MB452 also caused changes in tubulin and proteasome gene expression levels which may be linked to intestinal barrier function. Caco-2 tight junctions were visualised by fluorescent microscopy of immuno-stained occludin, zona occludens (ZO)-1, ZO-2 and cingulin. Caco-2 cells treated with <it>L. plantarum </it>MB452 had higher intensity fluorescence of each of the four tight junction proteins compared to untreated controls.</p> <p>Conclusions</p> <p>This research indicates that enhancing the expression of genes involved in tight junction signalling is a possible mechanism by which <it>L. plantarum </it>MB452 improves intestinal barrier function.</p

Testing gravitational-wave searches with numerical relativity waveforms: Results from the first Numerical INJection Analysis (NINJA) project

The Numerical INJection Analysis (NINJA) project is a collaborative effort between members of the numerical relativity and gravitational-wave data analysis communities. The purpose of NINJA is to study the sensitivity of existing gravitational-wave search algorithms using numerically generated waveforms and to foster closer collaboration between the numerical relativity and data analysis communities. We describe the results of the first NINJA analysis which focused on gravitational waveforms from binary black hole coalescence. Ten numerical relativity groups contributed numerical data which were used to generate a set of gravitational-wave signals. These signals were injected into a simulated data set, designed to mimic the response of the Initial LIGO and Virgo gravitational-wave detectors. Nine groups analysed this data using search and parameter-estimation pipelines. Matched filter algorithms, un-modelled-burst searches and Bayesian parameter-estimation and model-selection algorithms were applied to the data. We report the efficiency of these search methods in detecting the numerical waveforms and measuring their parameters. We describe preliminary comparisons between the different search methods and suggest improvements for future NINJA analyses.Comment: 56 pages, 25 figures; various clarifications; accepted to CQ

Cohasset Rain Garden Project

Cohasset is a South Shore community in which approximately 90 percent of the drinking water supply comes from the Lily Pond surface water treatment plant. Lily Pond is shallow and currently eutrophic. The reduction of nutrient loads and control of in-lake vegetation were identified as goals in the development of pond and water supply management strategy. Low Impact Development (LID) Techniques were instituted to treat roadway runoff within the water supply watershed prior to discharge to the pond. The stormwater Best Management Practices selected include bioretention cells (rain gardens) and vegetated bioretention swales. As a first step a demonstration rain garden was constructed at the Water Department parking lot – to serve as an educational tool for the community. Twenty rain gardens and two vegetated bioretention swales have been constructed so far within roadway rights-of-way. Twenty-nine additional rain gardens will be constructed in Spring of 2008. The runoff filters through specially engineered soil reducing pollutant and nutrient levels, reducing elevated temperatures of stormwater during summer months, and attenuating peak flows. The treated stormwater is collected by under-drains and returned to the stormwater system or infiltrated back into the ground. The Cohasset Rain Garden Project is an example of applying LID techniques to retro-fit existing drainage systems in a developed watershed. LID is an innovative approach to stormwater management involving site design that duplicates the hydrologic features of an undeveloped watershed. Instead of conveying, managing and treating stormwater in large, costly facilities located at the bottom of drainage areas, and often outside of the natural watershed, LID simulates natural hydrologic cycles by addressing stormwater through small, cost-effective landscape features located at the lot level. These landscape features include permeable paving, bio-retention cells / rain gardens, grass swales, filter strips, disconnected impervious areas, and cistern collection systems. Rain gardens represent a low-cost, low-maintenance technique to improve the quality of stormwater that enters the water supply