5 research outputs found
MOESM1 of Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Additional file 1. Detection of leucopelargonidin in S. cerevisiae strains IMX106-F and IMX106-FD. Leucopelargonidin is visible as a red-coloured band within the isoamyl alcohol phase after treatment with acidified butanol
MOESM4 of Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Additional file 4. LC–MS chromatograms of cell pellet extracts of S. cerevisiae strains PATW076 and IMK393. The strains were grown in a bioreactor and biomass samples were taken after the reconsumption of ethanol. Pelargonidin and pelargonidin 3-O-glucoside (P3G) were found in the PATW076 sample. Chromatogram characteristics: (A) m/z range 433.111-433.115, (B) m/z range 271.055-271.065
MOESM5 of Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Additional file 5. Table with primers used in this study. Table with primers used in this study
MOESM3 of Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Additional file 3. Growth and production time courses of extracellular product formation in S. cerevisiae strains PATW066, PATW079, PATW080 and PATW076 in shake flask cultures. The strains were grown in shake-flasks with 50 mL SMG and the OD600 and extracellular metabolite concentration of coumaric and phloretic acids, naringenin, dihydrokaempferol (DHK), kaempferol and kaempferol 3-O-glucoside (K3G) expressed in ¾M were measured by HPLC in supernatant of cultures in time
MOESM2 of Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Additional file 2. Growth and production time courses of extracellular product formation in S. cerevisiae strains PATW002, PATW011, and PATW012 in shake flask cultures. The strains were grown in shake-flasks with 50 mL SMNar (1.5 mM naringenin) and the OD600 and extracellular metabolite concentration of dihydrokaempferol (DHK), kaempferol and kaempferol 3-O-glucoside (K3G) expressed in ¾M were measured by HPLC in supernatant of cultures in time