Additional file 2. Growth and production time courses of extracellular product formation in S. cerevisiae strains PATW002, PATW011, and PATW012 in shake flask cultures. The strains were grown in shake-flasks with 50Â mL SMNar (1.5Â mM naringenin) and the OD600 and extracellular metabolite concentration of dihydrokaempferol (DHK), kaempferol and kaempferol 3-O-glucoside (K3G) expressed in ÂľM were measured by HPLC in supernatant of cultures in time