Siddall Appendix

Siddall Appendi

Multiple sequence alignment of the <i>Odontosyllis enopla</i> luciferase gene with that of the Japanese syllid <i>O</i>. <i>undecimdonta</i>.

<p>The <i>Odontosyllis enopla</i> luciferase gene (329 amino acids in length) is aligned with the four putative luciferase transcripts (isoforms) of <i>O</i>. <i>undecimdonta</i>. The alignment was generated using default parameters and the L-INS-i iterative refinement method within MAFFT (v7.402). Ou: <i>O</i>. <i>undecimdonta</i>. Oe1: <i>O</i>. <i>enopla</i> Individual 1.</p

Genes associated with putative epitoky-related changes of the eyes in female <i>Odontosyllis enopla</i> (Results reported for Individual #1 only).

<p>Genes associated with putative epitoky-related changes of the eyes in female <i>Odontosyllis enopla</i> (Results reported for Individual #1 only).</p

An unrooted maximum likelihood-based phylogenetic tree showing the relationship of both orthologs and paralogs of the luciferase gene for <i>Odontosyllis enopl</i>a and <i>O</i>. <i>undecimdonta</i>.

<p>The four transcripts (isoforms) found by Schultz et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200944#pone.0200944.ref044" target="_blank">44</a>] are in orange. ‘O_undecimdonta_DN31989’ (green) is identical to one of the four isoforms but has a different name because it is based on our Trinity assembly. The two additional green terminals are paralogs of the <i>O</i>. <i>undecimdonta</i> luciferase that were not reported by Schultz et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200944#pone.0200944.ref044" target="_blank">44</a>]. For <i>O</i>. <i>enopla</i>, orthologs are shown in purple and the paralogs in blue. O_enopla_1: Individual 1, O_enopla_2: Individual 2, O_enopla_3: Individual 3. The ML tree was constructed using a MUSCLE-based amino acid alignment and the following parameters: WAG + gamma + I model; aLRT-based support values.</p

Bioluminescent display of <i>Odontosyllis enopla</i>.

<p>During the breeding period, female <i>Odontosyllis enopla</i> swim in slow circles secreting a bright bluish-green luminous mucus while releasing gametes. Photo credit: Dr. James B. Wood.</p

Genes associated with putative epitoky-related changes of the nephridia in female <i>Odontosyllis enopla</i> (Results reported for Individual #1 only).

<p>Genes associated with putative epitoky-related changes of the nephridia in female <i>Odontosyllis enopla</i> (Results reported for Individual #1 only).</p

Chtonobdella tanae sp. n. holotype (AU81) from Queensland Australia, μCT projections.

Specimen fixed in 10% formalin and 70% ethanol for over a decade was refixed with AFA, stained in osmium tetroxide, and then scanned in a GE v|tome|x s240

Chtonobdella tanae sp. n. holotype (AU81) from Queensland Australia, μCT volumetric video rendering of external and internal features.

Video starts with whole-body (anterior to the right) external feature rendering viewed dorsally, laterally, and then ventrally, followed by whole-body internal feature rendering viewed dorsally, laterally, and then ventrally. Video zooms in on eyespots (green), jaws (magenta), ganglia (yellow), and gastric tissue (grey) viewed dorsally, laterally, and then ventrally. Video moves posteriorly, focusing on male median reproductive tissues (shades of blue), female median reproductive tissues (pink), and testisacs (red) viewed dorsally, laterally, and then ventrally. Video goes to the posterior focusing on intestinal tissue (pale green) viewed dorsally, laterally, and then ventrally. Video then focuses on the auricle cavities (blue), finishing on the dorsal view of whole-body internal feature

Juvenile Macrobdella decora, μCT projections.

Specimens were fixed first with either ethanol (small sized specimen on left), glutaraldehyde (large sized specimen in middle), or AFA (medium sized specimen on right); stained in osmium tetroxide; and then scanned in a GE v|tome|x s240

Codon usage for aspartic acid in Clitellata mitochondrial genomes.

<p>Number of codons present in proteins used to code aspartic acid in Clitellata mitochondrial genomes. Even though all Hirudinea, except for <i>P</i>. <i>lamothei</i>, have a strong bias for the use of the GAT codon, they exclusively code for the <i>trnD</i> gene with a GUC anticodon.</p