Demonstration of a multi-technique approach to assess glacial microbial populations in the field

The ability to perform microbial detection and characterization in-field at extreme environments, rather than on returned samples, has the potential to improve the efficiency, relevance and quantity of data from field campaigns. To date, few examples of this approach have been reported. Therefore, we demonstrate that the approach is feasible in subglacial environments by deploying four techniques for microbial detection: real-time polymerase chain reaction; microscopic fluorescence cell counts, adenosine triphosphate bioluminescence assay and recombinant Factor C assay (to detect lipopolysaccharide). Each technique was applied to 12 subglacial ice samples, 12 meltwater samples and two snow samples from Engabreen, Northern Norway. Using this multi-technique approach, the detected biomarker levels were as expected, being highest in debris-rich subglacial ice, moderate in glacial meltwater and low in clean ice (debris-poor) and snow. Principal component analysis was applied to the resulting dataset and could be performed in-field to rapidly aid the allocation of resources for further sample analysis. We anticipate that in-field data collection will allow for multiple rounds of sampling, analysis, interpretation and refinement within a single field campaign, resulting in the collection of larger and more appropriate datasets, ultimately with more efficient science return

Novelty Search for Deep Reinforcement Learning Policy Network Weights by Action Sequence Edit Metric Distance

Reinforcement learning (RL) problems often feature deceptive local optima, and learning methods that optimize purely for reward signal often fail to learn strategies for overcoming them. Deep neuroevolution and novelty search have been proposed as effective alternatives to gradient-based methods for learning RL policies directly from pixels. In this paper, we introduce and evaluate the use of novelty search over agent action sequences by string edit metric distance as a means for promoting innovation. We also introduce a method for stagnation detection and population resampling inspired by recent developments in the RL community that uses the same mechanisms as novelty search to promote and develop innovative policies. Our methods extend a state-of-the-art method for deep neuroevolution using a simple-yet-effective genetic algorithm (GA) designed to efficiently learn deep RL policy network weights. Experiments using four games from the Atari 2600 benchmark were conducted. Results provide further evidence that GAs are competitive with gradient-based algorithms for deep RL. Results also demonstrate that novelty search over action sequences is an effective source of selection pressure that can be integrated into existing evolutionary algorithms for deep RL.Comment: Submitted to GECCO 201

Orion Flight Performance Design Trades

A significant portion of the Orion pre-PDR design effort has focused on balancing mass with performance. High level performance metrics include abort success rates, lunar surface coverage, landing accuracy and touchdown loads. These metrics may be converted to parameters that affect mass, such as ballast for stabilizing the abort vehicle, propellant to achieve increased lunar coverage or extended missions, or ballast to increase the lift-to-drag ratio to improve entry and landing performance. The Orion Flight Dynamics team was tasked to perform analyses to evaluate many of these trades. These analyses not only provide insight into the physics of each particular trade but, in aggregate, they illustrate the processes used by Orion to balance performance and mass margins, and thereby make design decisions. Lessons learned can be gleaned from a review of these studies which will be useful to other spacecraft system designers. These lessons fall into several categories, including: appropriate application of Monte Carlo analysis in design trades, managing margin in a highly mass-constrained environment, and the use of requirements to balance margin between subsystems and components. This paper provides a review of some of the trades and analyses conducted by the Flight Dynamics team, as well as systems engineering lessons learned

The effect of Cichorium intybus and Lotus corniculatus on nematode burdens and production in grazed lambs

This report was presented at the UK Organic Research 2002 Conference. The study was designed to examine the hypothesis that chicory (Cichorium intybus) and Lotus sp. (Lotus corniculatus) have the potential to affect the naturally acquired nematode burden in grazed lambs. Organic male castrate lambs (48) with a naturally acquired parasite burden grazed replicate combination plots (0.6 ha) of chicory, Lotus corniculatus, perennial ryegrass (Lolium perenne) and white clover (Trifolium repens). Lamb performance was determined by weekly weight gain and condition score assessments. Nematode burden was assessed by individual lamb faecal egg count (FEC) before and after drenching (levamisole). The range of parasitic helminths present was assessed by faecal culture and by total worm counts performed on a proportion of the lambs at slaughter. Weekly pasture larval counts (PLCs) were conducted on the trial plots. A concurrent small plot study (6 x 1m2 replicates) of each of the forages used in the grazing trial was run to assess the potential effect of forage type on the development and survival of Teladorsagia circumcincta assessed by weekly PLCs. Preliminary data suggest that lambs grazing chicory or a combination of lotus and chicory had lower FECs than those grazing PRG/WC, however there was no significant difference in the total worm counts

The Dilution of the Clean Water Act

This Article argues that the zero discharge goal of the Clean Water Act is more than naive rhetoric. To the contrary, it is the Act\u27s raison d\u27être, and it is woven into the fabric of the Act\u27s operative provisions. So understood, the zero discharge goal can and should provide continuing guidance for EPA\u27s implementation of the Act

Propagation of optical excitations by dipolar interactions in metal nanoparticle chains

Dispersion relations for dipolar modes propagating along a chain of metal nanoparticles are calculated by solving the full Maxwell equations, including radiation damping. The nanoparticles are treated as point dipoles, which means the results are valid only for a/d <= 1/3, where a is the particle radius and d the spacing. The discrete modes for a finite chain are first calculated, then these are mapped onto the dispersion relations appropriate for the infinite chain. Computed results are given for a chain of 50-nm diameter Ag spheres spaced by 75 nm. We find large deviations from previous quasistatic results: Transverse modes interact strongly with the light line. Longitudinal modes develop a bandwidth more than twice as large, resulting in a group velocity that is more than doubled. All modes for which k_mode <= w/c show strongly enhanced decay due to radiation damping.Comment: 26 pages, 7 figures, 2 tables. to appear in Phys. Rev.

Can kunzea oil (Kunzea ambigua) control head lice (Pediculus humanus capitis)?

SUMMARYHead lice (Pediculus humanus capitis) infestations are a public health concern. The insecticidal properties of the Australian native plantKunzea ambigua(commonly known as tick bush) have been documented. In this study, we tested activity of kunzea oil (KO) against head lice throughin vitrobioassays. Head lice were exposed to filter paper treated with either KO, as either a 5 or 100% oil, or commercial formulations containing either permethrin or tea tree oil (TTO) for 120 min. Head lice exposure to KO, both as a 5 and 100% solution oil, resulted in 100% mortality within 120 min with a mean survival times of 17·1 and 34·8 min, respectively. There was no significant difference between the mean mortality of head lice exposed to 5% KO (17·1 ± 1·0; 95% CI: 115·2–19·0) and 5% TTO (21·2 ± 1·9; 95% CI: 17·4–25·1). This study revealed, for the first time, that KO holds great potential as an effective alternative to current active ingredients contained within commercial pediculicide formulations.</jats:p

Peripartum cardiomyopathy: diagnosis and management

No abstract available