Salmonella enterica subsp houtenae sorogrupo O:16 em um paciente HIV positivo: relato de caso

We described a case of salmonellosis in a 33-year old HIV-infected patient. The patient presented oral and esophageal candidiasis, intense epigastric and retrosternal pain. During the physical examination he was hypochloraemic, acyanotic, hypohydrated, anicteric and afebrile. Admittance laboratorial tests indicated: red cells 3.6 millions/mm³; hemoglobin, 10.1 g/dL; leukocyte count, 3,000/mm³, with 1% of eosinophils, 14% of non-segmented and 53% of segmented neutrophils and 31% of lymphocytes. The blood culture was positive for Salmonella enterica subsp houtenae serogroup O:16. This is probably the first human report of bacteremia due to Salmonella enterica subsp houtenae in Brazil associated to HIV-infected patient.Descreve-se um caso clínico de salmonelose ocorrido em paciente HIV positivo de 33 anos, portador de candidíase oral e esofágica, com intensa dor abdominal superior e dor retro-esternal. Ao exame clínico apresentou-se hipocorado, acianótico, hipohidratado, anictérico e afebril. A investigação laboratorial na admissão apresentou: hemácias, 3,6 milhões/mm³; hemoglobina, 10,1 g/dL; contagem de leucócitos, 3.000/mm³, com 1% de eosinófilos, 14% de bastões; 53% de neutrófilos segmentados e 31% de linfócitos. A hemocultura foi positiva para Salmonella enterica subsp houtenae sorogrupo O:16. Provavelmente, este é o primeiro relato de caso clínico humano com bacteremia causado por Salmonella enterica subsp houtenae no Brasil associado a paciente HIV-infectado

Análise epidemiológica de cepas bacterianas envolvidas em infecção hospitalar em um Hospital Universitário no Brasil

As infecções hospitalares representam um aumento na morbidade e mortalidade de pacientes internados, com significativo aumento no custo de internação hospitalar. Teve-se como objetivo fazer uma análise epidemiológica de casos de infecção hospitalar ocorridos num Hospital Universitário na cidade do Rio de Janeiro. Assim, foram analisadas 238 cepas isoladas a partir de 14 espécimens clínicos diferentes oriundos de 166 pacientes internados no período de 08 de 1995 a 07 de 1997. A idade média dos pacientes foi de 33,4 anos, 72,9% faziam uso de antimicrobiano antes de apresentar a cultura positiva, as patologias de risco mais comuns foram: Cirurgia (19,3%), HIV ou AIDS positivo (18,1%) e Patologia Pulmonar (16,9%). Foram identificadas 24 espécies bacterianas distintas, com predominância de S. aureus (21%) e P. aeruginosa (18,5%). Foram detectados 36% de MRSA (Methicilin Resistant S. aureus). Os Gram negativos apresentaram altos níveis de resistência para aminoglicosídeos e cefalosporinas. Foi detectado um surto de diarréia em berçário patológico, provocado pela Salmonella sorovar Infantis, com altos níveis de resistência para antimicrobianos e um plasmídio de alto peso molecular (98Mda), codificador do fator R.Hospital infections cause an increase in morbidity and mortality of hospitalized patients with significant rise in hospital costs. The aim of this work was an epidemiological analysis of hospital infection cases occurred in a public University Hospital in Rio de Janeiro. Hence, 238 strains were isolated from 14 different clinical materials of 166 patients hospitalized in the period between August 1995 and July 1997. The average age of the patients was 33.4 years, 72.9% used antimicrobials before having a positive culture. The most common risk conditions were surgery (19.3%), positive HIV or AIDS (18.1%) and lung disease (16.9%). 24 different bacterial species were identified, S. aureus (21%) and P. aeruginosa (18.5%) were predominant. Among 50 S. aureus isolated strains 36% were classified as MRSA (Methicillin Resistant S. aureus). The Gram negative bacteria presented high resistance to aminoglycosides and cephalosporins. A diarrhea outbreak, detected in high-risk neonatology ward, was caused by Salmonella serovar Infantis strain, with high antimicrobial resistance and a plasmid of high molecular weight (98Mda) containing virulence genes and positive for R factor

Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii

The aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse primers, we demonstrated that the ISAba1 element did not occur upstream of the blaOXA-23 gene in five of 31 isolates, which explained the lack of resistance to imipenem despite the presence of the blaOXA-23 gene. All of the blaOXA-23-positive isolates were susceptible to imipenem and meropenem with minimal inhibitory concentration < 4 µg/mL. Pulsed-field gel electrophoresis analysis revealed four genotypes among the five blaOXA-23-positive isolates. The current report of the blaOXA-23 gene in imipenem-susceptible isolates provided evidence that this gene may be silently spread in a hospital environment and highlighted the threat of undetected reservoirs of carbapenemase genes

A Salmonella Agona outbreak in a pediatric hospital in the ciry of Rio de Janeiro, Brazil

Submitted by Sandra Infurna ([email protected]) on 2019-06-13T12:08:36Z No. of bitstreams: 1 MariseAsensi_ClaudeSolari_etal_IOC_1994.pdf: 196759 bytes, checksum: 95c6cd6dcdf3f77b16ddf16c390d97b2 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-06-13T12:15:17Z (GMT) No. of bitstreams: 1 MariseAsensi_ClaudeSolari_etal_IOC_1994.pdf: 196759 bytes, checksum: 95c6cd6dcdf3f77b16ddf16c390d97b2 (MD5)Made available in DSpace on 2019-06-13T12:15:17Z (GMT). No. of bitstreams: 1 MariseAsensi_ClaudeSolari_etal_IOC_1994.pdf: 196759 bytes, checksum: 95c6cd6dcdf3f77b16ddf16c390d97b2 (MD5) Previous issue date: 1994Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Bacteriologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ. Brasil.Six Salmonella Agona strains from an outbreak of 15 days duration which occurred in a public hospital in Rio de Janeiro, Brazil, were analyzed. The outbreak involved six infants (mean age, 24 days; mean body weight, 1612 g), all of them with severe clinical signs and symptoms. Two of them had surgical implications, two were preterm and two had respiratory distress at birth. The Salmonella strains were resistant to nine antimicrobial agents (ampicillin, cephalotin, cefriaxone, gentamicin, amykacin, trimethoprim-sulfamethoxazole, chloramphenicol, and tetracyclin). Analysis of the plasmid pattern of the wild strains and of the transconjugants confirmed that these were identical strains

A Salmonella Agona outbreak in a pediatric hospital in the City of Rio de Janeiro, Brazil

Six Salmonella Agona strains from an outbreak of 15 days duration which occurred in a public hospital in Rio de Janeiro, Brazil, were analyzed. The outbreak involved six infants (mean age, 24 days; mean body weight, 1612 g), all of them with severe clinical signs and symptoms. Two of them had surgical implications, two were preterm and two had respiratory distress at birth. The Salmonella strains were resistant to nine antimicrobial agents (ampicillin, cephalotin, ceftriaxone, gentamicin, amykacin, trimethoprim-sulfamethoxazole, chloramphenicol and tetracyclin). Analysis of the plasmid pattern of the wild strains and of the transconjugants confirmed that these were identical strains

First Detection of the CTXM-15 Producing Escherichia coli O25-ST131 Pandemic Clone in Ecuador

Our aim was identify of the pandemic B2-ST131 Escherichia coli clone by to the Institute Pasteur and Achtman scheme, and investigate the resistance profile phenotypic-genotypic, with identification of class 1 integron. Of thirty-five ESBL-producing isolates recovered of patients with diagnosis of urinary tract infections (UTI), six E. coli strains serotype O25 were identified with resistance antimicrobial to several groups of antibiotics such as broad-spectrum cephalosporins, fluoroquinolones and aminoglycosides, harboring blaSHV, blaCTX-M genes in all isolates and blaTEM in two isolates. Sequencing of blaCTX-M revealed CTX-M-15 in all strains. The PMQR aac(6&acute;)-Ib-cr and qnrB19 genes were presented in five and four isolates respectively, AMEs genes aac(6&acute;)-Ib and aac(3)-IIa were presented in strain amikacin-gentamicin-resistant. Sequencing of the variable regions of the class 1 integron revealed dfrA and aadA genes cassette. The analysis of multilocus sequence typing (MLST) confirms the presence of the pandemic B2-ST131 E. coli clone by Achtman scheme in all ST43 isolates obtained by of the Institute Pasteur scheme. The results presented herein, reveal the presence of B2-ST131 E. coli clone in Ecuador, disseminated in hospitals and community settings