Genome-wide association study of placental weight identifies distinct and shared genetic influences between placental and fetal growth

A well-functioning placenta is essential for fetal and maternal health throughout pregnancy. Using placental weight as a proxy for placental growth, we report genome-wide association analyses in the fetal (n = 65,405), maternal (n = 61,228) and paternal (n = 52,392) genomes, yielding 40 independent association signals. Twenty-six signals are classified as fetal, four maternal and three fetal and maternal. A maternal parent-of-origin effect is seen near KCNQ1. Genetic correlation and colocalization analyses reveal overlap with birth weight genetics, but 12 loci are classified as predominantly or only affecting placental weight, with connections to placental development and morphology, and transport of antibodies and amino acids. Mendelian randomization analyses indicate that fetal genetically mediated higher placental weight is causally associated with preeclampsia risk and shorter gestational duration. Moreover, these analyses support the role of fetal insulin in regulating placental weight, providing a key link between fetal and placental growth

Genome-wide association study of placental weight identifies distinct and shared genetic influences between placental and fetal growth

A well-functioning placenta is essential for fetal and maternal health throughout pregnancy. Using placental weight as a proxy for placental growth, we report genome-wide association analyses in the fetal (n = 65,405), maternal (n = 61,228) and paternal (n = 52,392) genomes, yielding 40 independent association signals. Twenty-six signals are classified as fetal, four maternal and three fetal and maternal. A maternal parent-of-origin effect is seen near KCNQ1. Genetic correlation and colocalization analyses reveal overlap with birth weight genetics, but 12 loci are classified as predominantly or only affecting placental weight, with connections to placental development and morphology, and transport of antibodies and amino acids. Mendelian randomization analyses indicate that fetal genetically mediated higher placental weight is causally associated with preeclampsia risk and shorter gestational duration. Moreover, these analyses support the role of fetal insulin in regulating placental weight, providing a key link between fetal and placental growth

Genome-wide association study of placental weight in 65,405 newborns and 113,620 parents reveals distinct and shared genetic influences between placental and fetal growth

A well-functioning placenta is essential for fetal and maternal health throughout pregnancy. Using placental weight as a proxy for placental growth, we report genome-wide association analyses in the fetal (n = 65,405), maternal (n = 61,228) and paternal (n = 52,392) genomes, yielding 40 independent association signals. Twenty-six signals are classified as fetal, four maternal and three fetal and maternal. A maternal parent-of-origin effect is seen near KCNQ1. Genetic correlation and colocalization analyses reveal overlap with birth weight genetics, but 12 loci are classified as predominantly or only affecting placental weight, with connections to placental development and morphology, and transport of antibodies and amino acids. Mendelian randomization analyses indicate that fetal genetically mediated higher placental weight is causally associated with preeclampsia risk and shorter gestational duration. Moreover, these analyses support the role of fetal insulin in regulating placental weight, providing a key link between fetal and placental growth

Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop

<div><p>We recently reported the induction of potent, cross-clade neutralizing antibodies (nAbs) against Human Immunodeficiency Virus type-1 (HIV-1) in rabbits using gp120 based on an M-group consensus sequence. To better characterize these antibodies, 93 hybridomas were generated, which represent the largest panel of monoclonal antibodies (mAbs) ever generated from a vaccinated rabbit. The single most frequently recognized epitope of the isolated mAbs was at the very C-terminal end of the protein (APTKAKRRVVEREKR), followed by the V3 loop. A total of seven anti-V3 loop mAbs were isolated, two of which (10A3 and 10A37) exhibited neutralizing activity. In contrast to 10A3 and most other anti-V3 loop nAbs, 10A37 was atypical with its epitope positioned more towards the C-terminal half of the loop. To our knowledge, 10A37 is the most potent and broadly neutralizing anti-V3 loop mAb induced by vaccination. Interestingly, all seven anti-V3 loop mAbs competed with PGT121, suggesting a possibility that early induction of potent anti-V3 loop antibodies could prevent induction of more broadly neutralizing PGT121-like antibodies that target the conserved base of the V3 loop stem.</p></div

Locations of immunogenic peptides in the inner domain.

<p>A crystal structure of trimeric BG505 SOSIP gp140 (pdb: 4NCO) was used to illustrate locations of the immunogenic peptides. Only the gp120 portion is shown for clarity. The outer domain is shown in lime and the inner domain is shown in three shades of gray. A part of peptide 9094 and 9096 from the C5 region (indicated in a lighter magenta shade) is not shown in the crystal structure. The arrow points to the position of the five amino acids (VKIEP) on peptide 9094. For simplicity, the locations of the V3 and V5 loops are shown only on the side view.</p

Neutralizing activity of anti-V3 loop mAbs 10A3 and 10A37.

<p>V3 positive mAbs 10A37 and 10A3 were tested for neutralization against pseudoviruses belonging to different clades and tiers of HIV-1 and their IC₅₀ values (μg/ml) are shown. These mAbs were compared to a combination of two mAbs (CH01 and VRC-CH31) that are known to show broad cross-clade neutralization [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128823#pone.0128823.ref056" target="_blank">56</a>].</p

Epitope mapping analyses of hybridomas generated.

<p>Hybridomas were evaluated for reactivity against gp120, gp120-OD, BG505 SOSIP gp140, as well as eOD-GT6 and a large panel of peptides. Hybridomas are arranged in groups based on their linear epitopes or their reactivity to three proteins. NT: Not Tested.</p

Antibody competition against PGT121.

<p>(A) Culture supernatants of hybridomas specific against the V3 loop were evaluated for competing activity against PGT121 for binding gp120. The values on the x-axis represent supernatant concentration (1/dilution factor). (B) Recombinant mAb 10A37 was used for the competition assay. Anti-gp41, recombinant mAb 2C2 was used as a negative control.</p

Comparison of CDR regions of the anti-V3 loop mAbs.

<p>The heavy and light chains of the seven V3 loop-positive mAbs were aligned for analysis. Comparison was done based on peptide reactivity shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128823#pone.0128823.g006" target="_blank">Fig 6</a>. Percentages indicate % amino acid identity between the two CDR being compared.</p