Evaluation of participants' experiences with a non-restrictive minimally-structured lifestyle intervention. CHERE Working Paper 2010/11

While there is increasing evidence that group-based lifestyle-focussed interventions may provide more realistic, effective and cost-effective alternatives to intensive, individualised dietary counselling and exercise training, relatively little is known about individuals? preferences for and perceptions of these programs. This paper reports the results of qualitative interviews conducted with participants of a lifestyle intervention trial (Shape up for Life? (SufL) aimed to improve body composition and metabolic health through long-term non-restrictive behaviour modification. Purposive sampling was used to identify 22 participants who participated in detailed interviews regarding their expectations of the intervention, perceptions of benefits and their experience post-intervention and capacity to maintain the lifestyle changes. The results indicate that in general participants are focussed on weight loss as a goal, even when the intervention offered and provided other benefits such as improved fitness and body shape and composition. The individuals who benefited most from the intervention typically had lower baseline knowledge about dietary and exercise guidelines. While the relatively non-restrictive nature of SufL provided flexibility for participants, many participants perceived that a more structured program may have assisted in achieving weight loss goals.Obesity, lifestyle intervention, weight loss, metabolic syndrome

Musculoskeletal Sarcoidosis and Rheumatoid Factor

Articular manifestations of sarcoidosis were present in 19 of our 60 patients (32%). This correlates with previously reported studies. In all of our cases but one, the articular manifestations subsided in eight weeks. This fact and the absence of hyperuricemia are in contradistinction to other studies for which we have no adequate explanation

Owen Davis Correspondence

Entries include brief and some erroneous, biographical information, a handwritten letter on personal stationery, and a typed letter on Walter H. Baker Company, Dramatic Publications, stationery from Johnson

Food in the school curriculum in England: Its development from cookery to cookery

The view of the authors is that the teaching of food in the school curriculum has varied throughout its history in order to meet political aims rather than educational ones. In this article they highlight the social and political changes that have influenced the teaching of food from its inception in the mid-1840s through to the present day. They argue that the political influences have been detrimental to the value of teaching about food and its potential for contributing to pupils’ overall education as well as what pupils should know, understand and learn about food and where it can be taught in schools. The teaching of food as cookery is traced from its introduction in the elementary school system, when it was for girls only, then to its development into domestic science, a subject for more academically able girls and the Sex Discrimination 1975 ensuring its availability for boys and girls. This was followed by the transformation into home economics, with a wider curriculum agenda, in the 1970s, the introduction of higher education degrees and the National Curriculum in 1990, which put food technology within design and technology. Changes within the National Curriculum are considered as are recent events impacting on the teaching of food, up to 2015 when GCSE Food Technology was replaced with GCSE Food and Nutrition and A Level Food Technology, which supports pupil progression into a range of food related degrees and careers, was removed. The article reviews a range of literature in order to consider the value of teaching food, the current situation in schools in England and the possible future role of food in the school curriculum

The Influence of IL-10 and TNFα on Chondrogenesis of Human Mesenchymal Stromal Cells in Three-Dimensional Cultures

Chondrogenic differentiated mesenchymal stromal cells (MSCs) are a promising cell source for articular cartilage repair. This study was undertaken to determine the effectiveness of two three-dimensional (3D) culture systems for chondrogenic MSC differentiation in comparison to primary chondrocytes and to assess the effect of Interleukin (IL)-10 and Tumor Necrosis Factor (TNF)α on chondrogenesis by MSCs in 3D high-density (H-D) culture. MSCs were isolated from femur spongiosa, characterized using a set of typical markers and introduced in scaffold-free H-D cultures or non-woven polyglycolic acid (PGA) scaffolds for chondrogenic differentiation. H-D cultures were stimulated with recombinant IL-10, TNFα, TNFα + IL-10 or remained untreated. Gene and protein expression of type II collagen, aggrecan, sox9 and TNFα were examined. MSCs expressed typical cell surface markers and revealed multipotency. Chondrogenic differentiated cells expressed cartilage-specific markers in both culture systems but to a lower extent when compared with articular chondrocytes. Chondrogenesis was more pronounced in PGA compared with H-D culture. IL-10 and/or TNFα did not impair the chondrogenic differentiation of MSCs. Moreover, in most of the investigated samples, despite not reaching significance level, IL-10 had a stimulatory effect on the type II collagen, aggrecan and TNFα expression when compared with the respective controls

Rheumatoid Arthritis and Malignancy

A retrospective study of the incidence of rheumatoid arthritis and systemic malignancy was performed using the records of 196 older age patients with classic or definite rheumatoid arthritis. The results were compared to 125 patients in the same age group who had arthritis of a non-rheumatoid type. There was no difference in the incidence of malignancy in the two groups of patients. However, there was a positive correlation between the incidence of malignancy and the use of long term adrenocorticosteroid therapy

Experimental analysis of movements by prairie rattlesnakes, Crotalus viridis , during hibernation

Prairie rattlesnakes from Colorado, USA, were subjected to two thermal treatments during hibernation. The control treatment was carried out in a surrogate den in St. Louis, MO, USA and followed the normal seasonal thermal regime. Experimental manipulations were carried out in a second den in St. Louis. These series of manipulations reversed or modified the thermal gradient normally expected in wild dens. Periodic observations of the snakes within the control and experimental dens provided corroborative data to support the hypothesis that entry to and exit from dens is regulated by a reversing thermal gradient within wild dens.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47740/1/442_2004_Article_BF00344649.pd

Editorial: Neurobiology of spontaneous object exploration in recognition memory

This study was supported by the Brain and Behavior Research Foundation Young Investigator grant: 29192 to OC.Publisher PDFPeer reviewe

The pro-apoptotic K-Ras 4A proto-oncoprotein does not affect tumorigenesis in the ApcMin/+ mouse small intestine.

BACKGROUND: Alterations in gene splicing occur in human sporadic colorectal cancer (CRC) and may contribute to tumour progression. The K-ras proto-oncogene encodes two splice variants, K-ras 4A and 4B, and K-ras activating mutations which jointly affect both isoforms are prevalent in CRC. Past studies have established that splicing of both the K-ras oncogene and proto-oncogene is altered in CRC in favour of K-ras 4B. The present study addressed whether the K-Ras 4A proto-oncoprotein can suppress tumour development in the absence of its oncogenic allele, utilising the ApcMin/+ (Min) mouse that spontaneously develops intestinal tumours that do not harbour K-ras activating mutations, and the K-rastmDelta4A/tmDelta4A mouse that can express the K-ras 4B splice variant only. By this means tumorigenesis in the small intestine was compared between ApcMin/+, K-ras+/+ and ApcMin/+, K-rastmDelta4A/tmDelta4A mice that can, and cannot, express the K-ras 4A proto-oncoprotein respectively. METHODS: The relative levels of expression of the K-ras splice variants in normal small intestine and small intestinal tumours were quantified by real-time RT-qPCR analysis. Inbred (C57BL/6) ApcMin/+, K-ras+/+ and ApcMin/+, K-rastmDelta4A/tmDelta4A mice were generated and the genotypes confirmed by PCR analysis. Survival of stocks was compared by the Mantel-Haenszel test, and tumour number and area compared by Student's t-test in outwardly healthy mice at approximately 106 and 152 days of age. DNA sequencing of codons 12, 13 and 61 was performed to confirm the intestinal tumours did not harbour a K-ras activating mutation. RESULTS: The K-ras 4A transcript accounted for about 50% of K-ras expressed in the small intestine of both wild-type and Min mice. Tumours in the small intestine of Min mice showed increased levels of K-ras 4B transcript expression, but no appreciable change in K-ras 4A transcript levels. No K-ras activating mutations were detected in 27 intestinal tumours derived from Min and compound mutant Min mice. K-Ras 4A deficiency did not affect mouse survival, or tumour number, size or histopathology. CONCLUSION: The K-Ras 4A proto-oncoprotein does not exhibit tumour suppressor activity in the small intestine, even though the K-ras 4A/4B ratio is reduced in adenomas lacking K-ras activating mutations.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are