SNP Genotypes for native and introduced populations of Mimulus guttatus and outgroups

SNP genotypes at 1,400,000 loci for 10 native, 20 introduced, individuals of Mimulus guttatus, as well as 5 outgroups of Mimulus sect. Simiolus

SUPPLEMENTAL METHODS from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F2 crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F2s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate due to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Figure S5 from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F₂ crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F₂s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate owing to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Figure S1 from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F2 crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F2s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate due to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Figure S3 from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F₂ crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F₂s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate owing to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Figure S2 from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F₂ crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F₂s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate owing to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Figure S4 from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F2 crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F2s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate due to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

SUPPLEMENTAL METHODS from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F₂ crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F₂s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate owing to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Legends of the electronic supplementary pictures from Natural selection and outbreeding depression suggest adaptive differentiation in the invasive range of a clonal plant

Analyses of phenotypic selection and demography in field populations are powerful ways to establishing the potential role of natural selection in shaping evolution during biological invasions. Here we use experimental F2 crosses between native and introduced populations of <i>Mimulus guttatus</i> to estimate the pattern of natural selection in part of its introduced range, and to seek evidence of outbreeding depression of colonists. The F2s combined the genome of an introduced population with the genome of either native or introduced populations. We found that the introduced × introduced cross had the fastest population growth rate due to increased winter survival, clonality and seed production. Our analysis also revealed that selection through sexual fitness favoured large floral displays, large vegetative and flower size, lateral spread and early flowering. Our results indicate a source-of-origin effect, consistent with outbreeding depression exposed by mating between introduced and native populations. Our findings suggest that well-established non-native populations may pay a high fitness cost during subsequent bouts of admixture with native populations, and reveal that processes such as local adaptation in the invasive range can mediate the fitness consequences of admixture

Posterior probability of cluster assignment in nested InStruct analyses of 329 <i>Solanum rostratum</i> individuals genotyped at 10 microsatellite loci across 15 populations in three geographic regions: Mexico, the U.S.A. and China.

<p>Each bar represents a single individual with populations separated by black lines and arranged from North to South within each region. Panel <b>A</b> depicts the uppermost level of population structure across regions. The optimal number of clusters (<i>K*</i> = 2) was calculated using Evanno’s et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#B31" target="_blank">31</a>] <i>∆K</i> statistic. For illustration, panel <b>B</b> shows the assignment probabilities for <i>K</i> = 3. Panel <b>C</b> represent two separate analyses of either U.S.A./Chinese or Mexican populations; the optimal number of clusters [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#B31" target="_blank">31</a>] in each of these separate analyses was <i>K</i>_US-C<i>*</i> =<i>K</i>_US-C<i>*</i> = 2. Panel <b>D</b>, shows assignment probabilities for <i>K</i> = 3, for each separate analysis.</p